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Articles by Zubeda Chaudhry
Total Records ( 3 ) for Zubeda Chaudhry
  Hamid Rashid , Rizwana Abdul Ghani and Zubeda Chaudhry
  Effects of media, growth regulators and genotype were investigated for callus induction, maintenance and regeneration in wheat (Triticum aestivum L. cvs., Chakwal 86, Rawal 87). The percentage of callus induction in Rawal 87 was 49.58 -75.62% on Murashige and Skoog (MS) medium and 58.31-91.58% on N6 medium. For Chakwal-86, callus induction frequency ranged from 52.08- 81.04% on MS medium and 53.12- 81.02% on N6 medium supplemented with 2mg/l 2, 4 dichlorophenoxy acetic acid(2,4-D) singly, and with three concentrations (0.1, 0.5 and 1mg/L) of 6-Benzylaminopurine (BAP). BAP proved to play no role in callus induction. Sorbitol promoted callus proliferation, on N6 medium supplemented with 2mg/L 2,4-D. For plant regeneration from calli, MS medium with 0.1mg/L IAA and four concentrations (0.5, 1.0, 2.5 and 5mg/L) of BAP were tested. Regeneration frequency was greater (31.9%) in Rawal 87 on the medium containing 0.5mg/L BAP whereas 2.5mg/L of BAP were found suitable for regeneration in Chakwal 86 (15.27%).
  Muhammad Farooq , Hamid Rashid , Ihsanullah , Zubeda Chaudhry and Khan Bahadar Marwat
  Three wheat genotypes i.e. Bakhtawar-92, Punjab-96 and Inqilab-91 were tested for their response to callus induction frequency and their subsequent regeneration on a variety of media combinations. Bakhtawar-92 appeared to be the most responsive genotype to callus induction followed by Inqilab-91 and Punjab-96. It also produced significantly higher amount of callus as compared with other genotypes. However, the medium containing 2 mg l-1 2,4-D (2,4-dicholorophenoxy acetic acid) was found to be optimum for callus induction irrespective of the genotypes studied. Regeneration frequency of Bakhtawar-92 was 40% on the medium containing 0.1 mg l-1 IAA (Indole acetic acid) and 2.5 mg l-1 BAP (6-benzyl amino purine). Punjab-96 and Inqilab-91 showed regeneration of 25 and 33% on the medium supplemented with combination of 0.1 mg l-1 IAA and 0.5 mg l-1 BAP. Regenerated plants were evaluated for plant height, maturity and seed set. They had favourable significant differences from the control plants for the most important agronomic traits; plant height, days to maturity and kernels/spike etc.
  Hamid Rashid , Mehreen Saleem , Zubeda Chaudhry , S. Tallat Gilani and Asfari Sharif Qureshi
  Super Basmati is a commercial cultivar and is subjected to various stresses. This study was conducted to obtain high frequency regeneration from Super Basmati which is a pre-requisite for transformation protocol. Callus induction was obtained from N6 media with 2 mg l-1 2,4-D. The frequency of callus induction was 83.3% with N6 media and 75.05% on MS media. Regeneration of calli was the best on MS media with NAA 1 mg l-1 and BAP 5 mg l-1 i.e. 81.6%.
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