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Articles by Zongjun Yin
Total Records ( 4 ) for Zongjun Yin
  Shaoqian Cai , Yang Liu , Chenhua Zhang , Weixuan Fu , Yuanfang Gong , Xin Lu , Qin Zhang and Zongjun Yin
  This study was aimed to identify Quantitative Trait Loci (QTL) for haematological traits in pig chromosome 10 and facilitate the cloning of candidate genes underlying the QTLs. Hematological traits are essential parameters for evaluating the health status of animals and play a extremely important role in disease resistance. In this study, three main components, leukocyte traits, erythrocyte traits and platelet traits were measured in a composite pig population consisting of 445 pigs of three breeds (Landrace, Large White and Songliao Black Pig) distributed in 16 boar families, before and after vaccination with modified live CSF (classical swine fever) vaccine. A partial genome scan for mapping Quantitative Trait Loci (QTL) for these traits was performed by genotyping 13 microsatellite markers on chromosome 10. Through a linear mixed model and the permutation for empirical threshold values, 4 significant QTLs on chromosome 10 were identified affecting hematocrit (HCT), hemoglobin (HGB), Mean Corpuscular Volume (MCV) and blood platelet counts (PLT) (p<0.05), respectively. Our results confirms that haematological traits variation differs between the three pig breeds and variations of HCT, HGB, MCV, PLT are associated with the 81~133 cM region in chromosomal 10.
  Zongjun Yin , Gui Mei , Yang Liu , Xiangdong Ding and Qin Zhang
  Based on QTL fine mapping results on bovine chromosome 6 in the previous studies, KLF3 gene was chosen as a candidate gene to evaluate its effect on milk production traits in a Chinese Holstein population. A SNP (NC_007304:g.13849 G>C) in exon4 of KLF3 gene was demonstrated by directly sequencing of PCR product. Milk production traits including milk yield, fat yield, protein yield, fat percentage and protein percentage of each cow were collected by using 305 days lactation records. The further association analysis of the SNP genotype with milk production traits were conducted in a Chinese Holstein population including 1417 cows. We found that there were a SNP associated significantly with milk yield (p = 0.022) and protein yield (p = 0.033). The effect of CC genotype of SNP (NC_007304:g.13849 G>C) was significantly higher than other genotype (p<0.05) in both traits. The study suggested that the KLF3 gene could be used for marker assisted selection program as a molecular marker gene for milk yield, protein yield in Holstein dairy cattle breeding.
  Xiaoling Ding , Yan Yan , Chenghua Zhang , Yueyun Ding and Zongjun Yin
  In this study, leptin gene was chosen as a candidate gene to evaluate its effect on litter performance traits in four indigenous pig population of Anhui province in China. Two SNPs (U_66254 g.3469 T>C; g. 3714 G>T) in Exon 3 of leptin gene were demonstrated by PCR-SSCP and sequencing of PCR product. Litter performance traits including Numbers of Born Alive (NBA) of the first parity and latter parities were recorded in four pig breeds population. The further association analysis of the 2 SNPs with litter performance traits were conducted in four pig breeds population including 213 sows. Researchers found the 2 SNPs are significantly associated with litter performance traits. The effect of CC genotype of SNP (U_66254 g.3469 T>C) and TT genotype of SNP (U_66254 g.3714 G>T) were significantly higher than other genotypes in both litter traits (p<0.05). The study suggested that lepetin gene could be used for marker assisted selection program as a molecular genetic marker for litter sizes in Chinese indigenous pig breeding.
  Xiaoling Ding , Yueyun Ding , Chenghua Zhang , Long Huang , Xiaolei He and Zongjun Yin
  In order to give a basis for molecular breeding of meet quality traits in Wannan black pig, we applied PCR-RFLP method to analyze the polymorphism of 5'upstream region and intron 2 of H-FABP gene in 184 Wannan black pigs population. The results show that there exist polymorphic loci of restriction enzyme Hinf in 5'upstream region and Hae III as well as Hinf I in intron 2. Except for locus of restriction enzyme HinfI, the other two polymorphism sites achieved in Hardy-Weinberg equilibrium based on χ2-test (p>0.05). In addition, a T→C mutation in 1324 bp, a C→G mutation in 1811 bp and a C→T mutation in 1970 bp were found through the direct sequencing the polymorphic RFLP fragments of H-FABP gene, then genotype frequencies, gene frequencies, Ho, He, Ne and PIC were investigated in Wannan black pig population. The results identified 3 variant restriction sites and provided a basic data for further study of the relationship between IMF and H-FABP with different genotypes and also provide basic for make sure whether H-FABP gene is the key gene for effect on the deposition of IMF.
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