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Articles by Ziniu Yu
Total Records ( 3 ) for Ziniu Yu
  Suxia Guo , Mei Liu , Donghai Peng , Sisi Ji , Pengxia Wang , Ziniu Yu and Ming Sun
  We have developed a strategy for isolating cry genes from Bacillus thuringiensis. The key steps are the construction of a DNA library in an acrystalliferous B. thuringiensis host strain and screening for the formation of crystal through optical microscopy observation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses. By this method, three cry genes—cry55Aa1, cry6Aa2, and cry5Ba2—were cloned from rice-shaped crystals, producing B. thuringiensis YBT-1518, which consists of 54- and 45-kDa crystal proteins. cry55Aa1 encoded a 45-kDa protein, cry6Aa2 encoded a 54-kDa protein, and cry5Ba2 remained cryptic in strain YBT-1518, as shown by SDS-PAGE or microscopic observation. Proteins encoded by these three genes are all toxic to the root knot nematode Meloidogyne hapla. The two genes cry55Aa1 and cry6Aa2 were found to be located on a plasmid with a rather small size of 17.7 kb, designated pBMB0228.
  Guowei Zhou , Hui Huang , Ziniu Yu , Zhijun Dong and Yuanchao Li
  Free-living Symbiodinium have been the focus of considerable attention among coral reef researchers because they represent a source of symbionts for both recently recruited corals and adult corals that are subject to environmental stress (e.g. climate change). We evaluated the genetic diversity of free-living Symbiodinium in seawater collected from the Xisha Islands in the South China Sea using sequence analysis of the hypervariable region of the chloroplast 23S. We sequenced a total of 267 cloned gene fragments representing Symbiodinium in clades A, B, C, D, F, and G, of which 34 were distinct types. The diversity of free-living Symbiodinium types was high at all sites. The majority of Symbiodinium sequences belonged to clade C. There was no significant difference in Symbiodinium sequence types among sites or depths. Our results highlight the high diversity of free-living Symbiodinium pools in the coral reef environment and provide a foundation for future studies of the spatiotemporal distribution of Symbiodinium. Such information is critical to understand and predict the influence of symbiotic dinoflagellates on the adaptation of their host to environmental changes.
  Shengdong Zhu , Yuanxin Wu , Ziniu Yu , Haibao Tong , Dachang Cheng and Decheng Xie
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