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Articles by Zi-En Pu
Total Records ( 3 ) for Zi-En Pu
  Wei Li , An-Jun Liu , Yu-Zhen Sheng , Guo-Yue Cheng , Zi-En Pu and Ya-Xi Liu
  In order to exploiting new genetic resources for the wheat breeding, nine waxy genes (granule-bound starch synthase, GBSSI) were obtained from wild species, Aegilops tauschii (DD) by special primer PCR cloning. These sequences showed the higher similarity to the sequences of Wx-D1 locus in common wheat. The analysis of variation and alignment of sequences showed that all the sequences could be distinguished, although from the same species. Out of 35 variable sites of nucleotide in the whole sequences, twelve variable sites located on the region of exon and made six amino acid residues change. Most of variable sites located on transit peptide. Using neighbour-joining method, phylogenetic tree suggested that the sequences of waxy gene from Ae. tauschii were clustered closely with the sequences of Wx-D1 locus and far with the sequence of Wx-A1 and Waxy-B1 loci in Triticum L.. Moreover, Ae. tauschii showed more close relationship with Triticum L. and barley and had more close relationship with barley and far distance with rice, potato, pea and Arabidopsis. These results would contribute to the deep understanding of functional aspects and evolution of waxy gene and the improving of starch quality in common wheat.
  Wei Li , An-Jun Liu , Yu-Zhen Sheng , Zi-En Pu , Ya-Xi Liu and Guo-Yue Cheng
  In order to knowing the variation of starch synthase I (SSI) in Triticum L. and Aegilops and exploiting new gene resources for the improving of starch quality in common wheat, using PCR cloning, eight sequences of SSI genes were obtained from AS60 (DD, Aegilops tauschii), AS2255 (AABB, Triticum turgidum), SHW-L1 (AABBDD, synthetic hexaploid wheat) and Chuanmai 32 (AABBDD, Triticum aestivum), respectively. These sequences of SSI gene showed the higher conservative characterization between different materials. A total of 10 variable nucleotide bases and two substitutions of amino acid residues (Ser/Asn and Ala/Val) were observed in the cloning sequences. Alignment of all sequences, including SSI gene from T. aestivum, Hordeum vulgare, Brachypodium distachyon, Zea mays and Oryza sativa suggested that the sequences from Ae, tauschii, T. turgidum and synthetic hexaploid wheat, were more similar to SSI gene from common wheat and barley and far distance with sequences from Brachypodium distachyon, Zea mays and Oryza sativa. Using neighbor-joining method, phylogenetic tree including a total of 28 sequences could be clustered into four groups. Most of sequences of SSI from different species were clustered together and SSI gene showed the clear difference with other starch synthases, including Granule-bound Starch Synthase (GBSS), SSII, SSIII and SSIV. These results suggested that there was variation fro SSI gene in Triticum L. and Aegilops. It necessary to developing molecular markers for exploit genetic resources and the improving of wheat starch quality.
  Wei Li , An-Jun Liu , Yu-Zhen Sheng , Guo-Yue Cheng , Zi-En Pu , Ya-Xi Liu and Li Kong
  In order to exploit new genetic resources for the improving of starch quality of common wheat, the genetic diversity of null alleles of Granule-bound starch synthase I (waxy gene) was investigated by special PCR molecular markers in Triticum L. The results indicated that there was relative abundant genetic diversity of waxy alleles in all accessions. Accession AS2347, AS2356, AS2317 and AS2308 with null allele at Waxy-B1 locus and AS2310 and AS2335 with null alleles at Waxy-A1 and Waxy-B1, were observed in 81 landraces of Triticum turdigum L. from China. In 53 landraces of Triticum aestivum L. from Sichuan, China, eight accessions at Waxy-A1, Waxy-B1 and Waxy-D1 loci and accession AS1668 at Waxy-D1, were observed null alleles. In 29 Triticum macha, Accession PI361862 and PI572911 at three Waxy loci, PI572913 at Waxy-B1 and Waxy-D1, PI572910 at Waxy-A1 and Waxy-D1, PI 290507 at Waxy-B1 and PI572906 at Waxy-D1, respectively, were observed null alleles. Seven accessions with null alleles at Waxy-B1 locus was observed in 28 Triticum sphaerococcum. Specially, the accessions of two regions, Anyue in Sichuan, China and Georgia, had the high frequency of the mutations with null alleles of waxy gene. Landraces of Triticum aestivum L. with the high frequency of waxy wheat, could be considered as a unique genetic resource for improving of waxy wheat. These result suggested that the special molecular marker could be used reliably in evaluation of genetic resources and these mutations also could be directly used in the improving of common wheat.
 
 
 
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