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Articles by Zhuo-Yi Zhang
Total Records ( 1 ) for Zhuo-Yi Zhang
  Jia-Di Dai , Yu-Juan Li , Zhuo-Yi Zhang , Yong- Wu , Yi-Song Liu , Zhi-Liang Sun and Zhao-Ying Liu
  Background and Objective: Gelsemium elegans (G. elegans) has been used in Chinese folk medicine for the treatment of rheumatoid pain, skin ulcers and malignant tumors. The aims of the study were to compare cytotoxicity and mitochondrial effects between G. elegans alcohol extract and G. elegans acid water extract. Moreover, the difference on chemical components between Gelsemium elegans alcohol extract and acid water extract was analyzed and compared in order to explore which components were responsible for the activity. Materials and Methods: The dried root of Gelsemium elegans was extracted with 95% alcohol and 0.5% sulphuric acid to get Gelsemium elegans alcohol extract and Gelsemium elegans acid water extract, respectively. In cytotoxic experiments, cell viability, apoptosis and production of reactive oxygen species (ROS) were measured. The extent of mitochondrial damage was assessed by measuring the mitochondrial membrane potential and intracellular Ca2+. The chemical components of the two different Gelsemium elegans extracts were characterized using LC-QqTOF MS method. Results: Viability experiments showed that G. elegans alcohol extract was more cytotoxic than the G. elegans acid water extract one. Both G. elegans alcohol extract and acid water extract induced apoptosis in HepG2 cells. The production of ROS was increased with increasing concentration of G. elegans extracts. Loss of mitochondrial membrane potential was detected after treatment with G. elegans extracts. The concentration of intracellular Ca2+ was significantly elevated at the concentration of 240 μg mL–1. Furthermore, a total of 29 and 21 components have been tentatively identified from the G. elegans alcohol extract and acid water extract, respectively. Conclusion: The present results demonstrated that oxidative stress and mitochondrial dysfunction induced by G. elegans extracts were the possible mechanism of cytotoxicity. Alkaloids and iridoids were the major constituents and possibly the cytotoxicity of the active fractions of G. elegans extracts.
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