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Articles by Zhiwen Xu
Total Records ( 6 ) for Zhiwen Xu
  YaLin He , Mengliang Liu , XiaoDi Wang , ShanShan Xu and Zhiwen Xu
  Prokaryotic expression vector of porcine stratifin was constructed to express stratifin in Escherichia coli (E. coli) and eukaryotic expression vector was also constructed. Stratifin cDNA was amplified using the PCR and cloned into pET32a (+) vector to form the recombinant plasmid SFN-pET32a (+); the recombinant plasmid SFN-pET32a (+) was then transformed into prokaryotic expression host E. coli Rosetta using lactose induction, the target protein was purified by using his affinity chromatographic separation. The recombinant protein stratifin was identified by SDS-PAGE. Stratifin cDNA was inserted into pcDNA3.1 (+) to form the recombinant plasmid SFN-pcDNA3.1 (+) and then it was identified by restriction enzyme digestion and DNA sequencing. The recombinant plasmid stratifin was successfully constructed, the recombinant stratifin protein was induced and eukaryotic expression vector was constructed. This study successfully realizes the construction of the prokaryotic and eukaryotic expression vectors and it could be used as a foundation for the future research on the function of this protein.
  Zhicai Zuo , Shan Liao , Xinqiao Qi , Yue Yi , Zhiwen Xu , Ling Zhu , Wanzhu Guo and Hengmin Cui
  BCL-10 is an important functional and an apoptotic regulator gene inducing the organismic innate immunity. It has not been reported that the BCL-10 protein of porcine in normal level could motivate immune factor to produce. Bioinformatics analysis was performed to predict the characteristics of BCL-10, the results indicated that BCL-10 gene encoded a polypeptide, molecular mass of 45 Ku and comprising 233 amino acids. The prediction of secondary structure showed that BCL-10 had 9 alpha-helix, 1 beta-sheet, 12 turn and 14 random coil. The protein had twenty transmembrane regions, an CARD structure field which located in 15-100 amino acids and no signal peptide, the hydrophobic index was between -2.500 and 1.400. The phylogenetic tree showed that BCL-10 was similar to human (the homology index was >95%) and had a distant relationship to mouse. To identify the activity of BCL-10, the protein with a His-tag was expressed in E. coli Rosetta and purified. The purified protein showed an obvious effect of cell proliferation. Cloning and analysising of BCL-10 gene which laid foundation to further research and exploitation.
  Desheng Li , Lei Chen , Chengdong Wang , Hongbo Dai , Mengliang Liu , Ling Zhu , Xiaoyu Wang , Zhiwen Xu , Wanzhu Guo and Heng Min Cui
  The nasal bacterial flora of seven giant pandas was identified by 16S ribosomal RNA (rRNA) gene sequencing analysis. The most widely occurring species were Staphylococcus and Escherichia coli. Several species not known to be associated with giant pandas were present in the study including Pantoea agglomerans, Corynebacterium auriscanis, Kurthia gibsonii and Corynebacterium glutamicum.
  Zuo Zhicai , Hongbo Dai , Lei Chen , Yuancheng Zhou , Wanzhu Guo and Zhiwen Xu
  Haemophilus parasuis mainly affect piglets during suckling period and the infected pigs showed clinical symptoms at 5-6 weeks old after weaning. To develop a specific PCR for early detection of Haemophilus parasuis, early treatment would effectively prevent phenotypic expression of the disease. A pair of specific primers were designed to amplify a 414 bp specific DNA fragment based on H. parasuis Outer Membrane Protein (OMP) gene published in GenBank also the PCR conditions were optimized. The established PCR test could detect a minimum of 425 cfu mL-1 (0.425 cfu/PCR reaction) pure cultures of H. parasuis and only H. parasuis could amplify a 414 bp specific DNA fragment. No amplification was observed while tested with the closely affiliated species and the mainly contaminating flora. From 2 days post infection (dpi) to 5 dpi, all of the piglets challenged with H. parasuis showed a fever above 41°C and all blood samples from those feverish piglets were positive for H. parasuis. Through blood PCR detection, 37 positive samples were picked out in 84 sucking pigs which were natural infected with H. parasuis, the result was verified by dissecting five positive piglets and isolating bacteria from lung tissue. The developed PCR was successfully applied for early diagnosis of H. parasuis infection, early treatment will reduce the incidence and mortality of disease.
  YaLin He , Mengliang Liu , XiaoDi Wang , ShanShan Xu and Zhiwen Xu
  Prokaryotic expression vector of porcine stratifin was constructed to express stratifin in Escherichia coli and Eukaryotic expression vector was also constructed. Stratifin cDNA was amplified using the PCR and cloned into PET32a (+) vector to form the recombinant plasmid SFN-PET32a (+) the recombinant plasmid SFN-PET32a (+) was then transformed into prokaryotic expression host E. coli Rosetta using lactose induction; the target protein was purified by using His affinity chromatographic separation. The recombinant protein stratifin was identified by SDS-PAGE. Stratifin cDNA was inserted into PCDNA3.1 (+) to form the recombinant plasmid SFN-PCDNA3.1 (+) and then it was identified by restriction enzyme digestion and DNA sequencing. The recombinant plasmid stratifin was successfully constructed, the recombinant stratifin protein was induced and eukaryotic expression vector was constructed. This study successfully realizes the construction of the prokaryotic and eukaryotic expression vectors and it could be used as a foundation for the future research on the function of this protein.
  Yingzhi Wang , Lei Chen , Desheng Li , Ling Zhu , Wanzhu Guo and Zhiwen Xu
  A Gram-positive coccus isolated from a Giant Panda was identified by 16S ribosomal RNA (rRNA) gene sequencing analysis. Phylogenetic analysis showed that the organism was a member of the family Rothia. The 16S rRNA gene sequence of the organism showed 100% similarity with R. nasimurium strain CCUG 35957. Animal experiments were conducted to study the pathogenicity of R. nasimurium SCGP. This is the first reported isolation of R. nasimurium from the upper respiratory tracta of Giant Panda.
 
 
 
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