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Articles by Zhengzhu Liu
Total Records ( 2 ) for Zhengzhu Liu
  Zhengzhu Liu , Yuanfang Gong , Wenjin Zhu , Lingxin Duan , Muxiang Ge , Baochang Shi , Minshan Feng and He Gao
  In order to explore the sequence structure of fox Agouti gene and it’s mechanism to regulate the pelage color’s dividing. In this study, the major part of intron 2 sequence (1038 bp) of Agouti gene from the silver fox were obtained by PCR amplification and direct sequencing. This sequence was aligned with red fox, giant panda, horse, pig, goat, cattle, sheep, domestic cat and rabbit and the sequence similarities were 100, 85.82, 75.75, 73.31, 66.22, 65.98, 65.77, 60.45 and 58.82%, respectively. The result of the homology analysis showed that the genetic relationship between silver fox and red fox was the highest, which was consistent with that they belong to Vulpes of the Canidae animal in traditional classification. Based on the sequence of Agouti gene intron 2, the phylogenetic tree was constructed for silver fox and the other 9 species using Clustalx (1.83) software. The cluster result of phylogenetic tree of all species was basically consistent with the taxonomy of NCBI and was similar to the physiological characteristics of the species and their traditional classification. The above results provide the important biological information for researching the mechanism of the formation mechanism of the coat color and artificially improving the coat color quality of fox and so on.
  Zhengzhu Liu , Yuanfang Gong , Lingxin Duan , Minshan Feng , Wenjin Zhu , Muxiang Ge , Xierong Liu , Junsheng Zhao and Xinling Guo
  To explore the sequence structure of fox TYR gene and it’s mechanism to regulate the pelage color’s dividing. In this study, the partial DNA sequence (1370 bp) of silver fox TYR gene was obtained by PCR amplification and direct sequencing. This sequence contained the intron 1 of 368 bp, the complete exon 2 of 217 bp and the intron 2 of 785 bp and GC content was 40.5%. Based on the sequences of TYR gene exon 2, the homological analysis and the construction of un-rooted phylogenetic tree were carried out among silver fox and the other 16 species by using DNAMAN software and Clustalx (1.83) software, respectively. The homological analysis showed that silver fox had the closest genetic relationship with dog and the closer genetic relationship with domestic cat and domestic ferret. The cluster result of un-rooted phylogenetic tree was similar to that of the homological analysis. The length of TYR gene exon 2 were 217 bp for all analyzed species, which indicated that TYR gene exon 2 were highly conservative in phylogeny. The above results can provide the important biological information for further researching the development and regulation mechanism of fox coat color which result from TYR gene.
 
 
 
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