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Articles
by
Zhen-Hua Liang |
Total Records (
2 ) for
Zhen-Hua Liang |
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Zhen-Hua Liang
,
Zheng-Zheng Li
,
Hong-Liang Huang
and
Yun-Jun Liu
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Two new ruthenium(II) complexes, [Ru(dmp)2(APIP)](ClO4)2 (1) (APIP = 2-(2-aminophenylimidazo[4,5-f][1,10]phenanthroline), dmp = 2,9-dimethyl-1,10-phenanthroline) and [Ru(dmp)2(HAPIP)](ClO4)2 (2) (HAPIP = 2-(2-hydroxyl-5-aminophenyl)imidazo[4,5-f][1,10]phenanthroline), were synthesized and characterized. The DNA-binding properties of these complexes were investigated by absorption titration, viscosity measurements, and photoactivated cleavage. The DNA-binding constants for 1 and 2 have been determined to be 2.3 (± 0.3) x 104 (mol L−1)−1 and 3.3 (± 0.4) x 104 (mol L−1)−1. The results indicate that 1 and 2 interact with DNA through intercalative mode. The cytotoxicities of 1 and 2 were assessed against BEL-7402, HepG-2 and MCF-7 cell lines using standard MTT assay. The apoptosis induced by these complexes was studied with the acridine orange/ethidium bromide staining method. The antioxidant activity on hydroxyl radical was also investigated. |
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Xian-Lan Hong
,
Zhen-Hua Liang
and
Mao-Hua Zeng
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Two triazine-containing ruthenium(II) complexes, [Ru(dmb)2(pdpt)](ClO4)2 (dmb = 4,4′-dimethyl-2,2′-bipyridine, pdpt = 3-(pyridine-2-yl)-5,6-diphenyl-as-triazine) (1) and [Ru(dmb)2(pdtp)](ClO4)2 (pdtp = 3-(pyridine-2-yl)-as-triazino[5,6-f]phenanthrene) (2), were synthesized and characterized by elemental analysis, ES-MS, 1H NMR, UV-Vis spectra, and cyclic voltammetry; the crystal structure of 2 was determined by X-ray diffraction. DNA-binding behaviors of both complexes were studied by absorption titration, thermal denaturation, and viscosity measurements. The photoactivated DNA cleavage of plasmid pBR322 in the presence of 1 and 2 was explored through gel electrophoresis. Antioxidant activities of the two complexes against hydroxyl radical (·OH) were also investigated. The results indicate that the DNA-binding affinity of 2 is much greater than that of 1, and that 2 binds to calf thymus DNA by intercalation, but 1 binds in partial intercalation, with π−π stacking on the DNA surface. Experimental findings made through analysis of the CV, absorption spectra, X-ray crystal structure, and the DNA-binding properties were compared with those of theoretical studies using density functional calculations. |
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