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Articles by Zhang Yong
Total Records ( 6 ) for Zhang Yong
  Abid Khan , Xiamu Niu and Zhang Yong
  A data integrity solution for mobile agents is presented. The proposed scheme combines digital watermarking and digital signature to achieve the desired security requirements of strong forward integrity, truncation resilience and non-repudiation. The results computed at each hop are first watermarked and then digitally signed. When the agent returns to the home platform we verify the signature first and then extract watermark. Any tampering with the results can be determined in the verification process. We have implemented the proposed scheme with various hashing algorithms like SHA-1, SHA-256 and SHA-512 for a price comparison scenario. For digital signature we have used RSA based signature. We have applied t-test to check the significance of present results. Present experimental results suggest that it can be used in an e-commerce application.
  Hu Junjie , Zhang Yong , Wang Junying , Zhao Xingxu and Zhang Hairong
  It is well known that melatonin is a coordinating signal for mammalian reproduction. In order to confirm the presence of melatonin receptors in hypothalamus-pituitary-gonadal axis and pineal of female Bactrian camel, the researchers used a Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) procedure to examine receptor MT1 expression. The length of MT1 gene was 452 bp. RT-PCR assaying revealed the presence of the mt1 (Mel1a) melatonin receptor subtype in reproduction axis and pineal which were obtained from the slaughterhouse in the Ningxia Autonomous Region, China. Sequence has been confirmed a high identity (above 85%) with melatonin receptor MT1 of other mammal known in GenBank. Comparing with other tissue’s sequences, one base substitution changed the 108th TTC codon (encoding Phenylalanine) to TAC (Tyrosine) on hypothalamus. Although, there are base substitutions in pineal’s gene, encoded amino acid are coincident with pituitary and ovary. The current results, the expression of MT1 receptor mRNA in brain and ovary, suggest that melatonin regulate reproduction function through not only neuroendocrine but also directly acting on the ovary in Bactrian camel.
  Fan Jie , Zhang Yong and Zhao Xingxu
  A Streptococcus agalactiae GS was isolated from milk from mastitis cattle in Gansu region of China. Sequence and phylogenetic analysis of Sip gene segments revealed that the Streptococcus agalactiae GS was most similar to a recent group B streptococcus isolated in dairy cattle from China. The Sip gene were selected and amplified, it contained a 1305 bp Open Reading Frame (ORF) which encoded 434 amino acids. The molecular mass of the deduced amino acid sequences was 56 kD. Blast analysis showed that it shared high identities (91.0-99.0%) with Sip sequences of other GBS registered in GenBank while lower identities (<50%) was found compared with other species of Streptococcus. The deduced amino acid homology was 90.2-98.8%.
  Du Ting-Yi , Zhang Yong and Zhang Yun
  Trefoil factor (TFF) family is a group of peptides with one or several trefoil factor domains in their structure, which are highly conserved in evolution, and are characterized by heat and enzymatic digestion resistance. The mammalian TFFs have three members (TFF1-3) , and the gastrointestinal tract and the airway system are major organs of their expression and secretion. At certain physiological conditions, with a tissue-specific distribution,TFF plays an important role in mucosal protection and wound healing. But in the malignant tissues, TFF is widely expressed, correlated strongly with the genesis, metastasis and invasion of tumor cells. These phenomena indicated that TFF may be a possible common mediator of oncogenic responses to different stimuli. The biological functions of TFF involve complex regulatory processes. Single chain TFF may activate cell membrane receptors and induce specific signaling transduction. On the other hand, TFF can form a complex with other proteins to exert its biological effects. In clinical medicine, TFF is primarily applied as drugs in the mucosal protection, in the prevention and the treatment of mucosal damage-related diseases and as pathological biomarkers of tumors. At present the first hand actions and the molecular mechanisms related to TFFs are still the major challenges in TFF research. Furthermore, the discovery of the naturally occurring complex of TFF and crystallins is highly valuable to the understanding of the biological functions and action mechanisms of TFF.
  Yu guo-yu , ZHANG Yong , JIANG Ping , Lee Wen-Hui and Zhang Yun
  Bm-TFF2, an amphibian trefoil factor, which is isolated from skin secretions of frog Bombina maxima, has much stronger biological activities than human TFFs. In the present study, Bm-TFF2 gene was amplified by polymerase chain reaction (PCR) from its cDNA and cloned into Pichia pastoris expression vector pPIC9K containing AOX1 promoter and α-factor leader sequence. Multi-copies insertion transformants were screened on G418 plates. After the induction by 1% methanol for 72 hours, the expression of Bm-TFF2 came up to the best quantity which was about 50 mg in 1L medium, and 80% saturation ammonium sulfate was suitable to collect the Bm-TFF2 protein, as identified by SDS-PAGE and Western blotting assay. The results showed that the plasmid of Bm-TFF2-pPIC9K was constructed successfully and expressed abundantly in eukaryotic expression system, which lies basis for researching further the biological activities and the relationship of structure and functions of Bm-TFF2.
  Yu Guo-Yu , XIANG Yang , ZHANG Hong-Yun , JIANG Ping , Lee Wen-Hui , ZHANG Yun and ZHANG Yong
  Bm-TFF2, a trefoil factor from the large-webbed bell toad (Bombina maxima), can stimulate cell migration and inhibit cell apoptosis. To study the structure-function relationship of Bm-TFF2, we constructed wild-type and mutated Bm-TFF2 plasmids and expressed recombinant proteins in E. coli. The wild-type Bm-TFF2 gene encoding mature peptide was obtained by RT-PCR, while the N-terminal, C-terminal and two arginine mutated Bm-TFF2 clones were constructed, and ligated into pET-32a(+) expression vectors. The fusion proteins were induced by IPTG at 37°C. The mutant Bm-TFF2 fusion proteins expressed mainly in the inclusion bodies. The mutant (TRX)/Bm-TFF2 could be purified by using Ni2+-chelating chromatography and reverse-phase HPLC from the inclusion body supernatant. The fusion proteins were analyzed by SDS-PAGE and Western blotting. The yield of mutant Bm-TFF2 fusion proteins of above 95% purity was about 20 mg/L. All three recombinant mutant proteins can promote the migration of AGS cells in a dose-dependent manner with no obvious activity difference.
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