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| Articles
by
Zargham Sepehrizadeh |
Total Records (
2 ) for
Zargham Sepehrizadeh |
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Eshrat Gharaei-Fathabad
,
Mojtaba Tabatabaei Yazdi
,
Seyed-Naser Ostad
,
Shadman Shokravi
,
Zargham Sepehrizadeh
,
Mohammad Ali Faramarzi
and
Mohsen Amini
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A total of 100 supernatants and methanol extract samples obtained from three weeks’
incubation of 50 different isolate strains of cyanobacteria with paddy-fields origin were investigated for their cytotoxicity properties. Fifteen supernatants and 10 methanol extract samples exhibited cytotoxic activity against Balb-C cells while one methanol extract sample possessed a cytotoxic effect against five different cell lines including HeLa, Vero, Caco-2, HepG-2 and CHO more than vincristine, 5-fluorouracil and methotrexate when used at their IC50 concentrations. The later potent extract was achieved from the cyanobacterium strain GT-319. It was characterized as Nostoc piscinale Kützing ex Bornet and Flahault 1888 according to the morphological studies. Identification of the species was performed following molecular taxonomy by 16S rRNA sequencing. This is the first report on the cytotoxic activity of N. piscinale GT-319. |
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Gholamreza Zarrini
,
Zargham Sepehrizadeh
,
Mojtaba Tabatabaei Yazdi
,
Qorbane Behzadiyan Nejad
and
Zuhair Muhammad Hassan
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Brucella abortus ribosomal L7/L12 protein is a 12 kDa protein encoded by rplL. This gene was amplified by PCR from B. abortus S19 and cloned in pET16b expression vector. The construct was transformed in Escherichia coli BL21 (DE3) PlysS. The expression was performed by addition 1 mM IPTG (isopropyl-beta-D-thiogalacto- pyranoside) in the medium with OD = 0.6 and incubation continued for 1, 2, 4 and 8 h at 37°C. The highest production (23 mg L-1) level was achieved after 4 h incubation of induction. The recombinant protein was purified by Ni-NTA column and characterized by SDS-PAGE. |
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