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Articles by Zamri Ishak
Total Records ( 3 ) for Zamri Ishak
  Nagi A. AL-Haj , Mariana N. Shamsudin , Raha A. Rahim and Zamri Ishak
  Although, PCR methods aimed on the detection of genes associated with the pathogenicity of Escherichia coli have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 E. coli isolates of various sources. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary OPAE-10 primer was used. After sequencing this fragment primer called secD was designed to be used as PCR primer. secD primer pairs was highly specific to detect all isolates including E. coli O157: H7.
  Nagi A. Al-Haj , Mariana N. Shamsudin , Raha A. Rahim and Zamri Ishak
  Quantitation assay of Escherichia coli, Samonell sp. and Vibrio cholerae cells investigated by exploiting the component consistently present on the outer surface of Gram-negative bacteria. In this study, a simple marine biolysate-based method for simultaneous detection of the gram negative pathogenic bacteria based on lipopolysaccharide component was optimized. The detection technique focused on the surface of these bacterial species, which is covered by polysaccharides and has high affinity to marine biolysate. E. coli, Samonell sp. and V. cholerae with similar initial cell count per mL have different but consistent absorbance readings by using the spectrophotometer and turbidity meter. This revealed that different genera of Gram Negative bacteria can be directly differentiated through standard curve that is plotted from the carbohydrate and marine biolysate assays absorbance readings. Both the assays elucidated a qualitative and quantitative detection of the pure culture pathogens.
  Nagi A. Al-Haj , Mariana N. Shamsudin , Raha A. Rahim and Zamri Ishak
  Recent advances in molecular techniques have revolutionized the detection of microorganism. The development of a molecular-based technique for detection of the three different targets of Enterbacteriacae was undertaken. Primer and probe were designed based on specific pepted of novel hemolymph protein of horseshoe crabs (Factor C anti-LPS) Tachypleus tridentatus that is believed to be involved in the binds to the lipopolysaccharide of Escherichia coli, Salmonella and Vibrio cholerae. The aim of our study the exploit part of cell wall polysaccharide in the development of improved detection method based on molecular approaches. In the gene detection assay, Lipopolysaccharide gene of Salmonella, V. cholera and E. coil were hybridized to anti-LPS factor gene found in the biolysate of the marine animals. The wzm and wzt genes encoding O-polysaccharide genes were amplified in these pathogens and the LPS factor C were amplified from the marine lysate. Development of a PCR-based technique for detection of the food-borne pathogens particularly Sa Salmonella, V. cholera and E. coil were achieved. Thus rapid, sensitive and reliable techniques for the detection of food-borne pathogens developed.
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