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Articles by Zamberi Sekawi
Total Records ( 4 ) for Zamberi Sekawi
  E. Amghalia , Nagi A. AL-Haj , Mariana N. Shamsudin , Nurmas I. Mashan , V. Neela and Zamberi Sekawi
  Methicilin Resistant Staphylococcus aureus (MRSA) implicated in many post-surgical and cancer treatment as well hospital and community fatalities need to be treated with an effective alternative antimicrobial agent. In the search for anti-MRSA agent, 2 types of natural products were investigated for inhibitory activity against MRSA. In addition to the bioassay, the activity of the anti-MRSA agent was elucidated based on the effect of both natural products on nucleotide changes of chromosome-encoded genes. In this study, the methanol extract of the red marine algae and the natural pure honey were studied for its antibacterial property based on disc diffusion test and Minimum Inhibitory Concentration (MIC). The effects of both natural products on selected gene sequences of S. aureus’s were determined by RT-PCR analysis. The genes of interest, which have been chosen in this study, are genes that are involved in the antibacterial mechanism including inhibition of cell wall synthesis, protein synthesis and nucleic acid synthesis. Five genes of interest chosen in this study include mecA gene, mecR1 gene, mecI gene, adaB gene and sav1017 gene. The results for antibacterial property showed the methanol extract of a red seaweed and the pure honey, inhibited growth of S. aureus strain according to the inhibition zones around discs saturated with the seaweed extract and pure honey, respectively. The MIC test showed decrease in growth of MRSA isolates after growing in broth incorporated with the extract and honey, respectively. The effect of the inhibitory activity of the natural products on selected gene sequences showed that several nucleotide changes occurred in the sequences of certain genes of interest based on the gene sequences of the cDNA after RT-PCR was carried out on the mRNA of S. aureus treated with the natural products. This research underlined that the inhibition effect of the natural products may be chromosome mediated evidenced by the changes of chromosome-encoded genes. The significant findings on activities of the seaweed extract and pure honey may become very useful in the process to find a better treatment for S. aureus infection especially, for the multiple drug resistant isolates. In addition, it is also, a new finding for natural product discovery through gene-expression analysis.
  Nagi A. Al-Haj , Nurmas I. Mashan , Mariana N. Shamsudin , Habsah Mohamad , Charles S. Vairappan and Zamberi Sekawi
  The in vitro antibacterial activities of seaweed belong to Euchema denticulatum extract showed inhibitory activity only on gram positive organisms tested including Staphylococcus aureus and Streptococcus pyogenes, which were expressed in terms of minimum inhibitory concentration and minimum bactericidal concentration test. Thus, gram negative pathogens tested including Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa showed resistant phenotypic pattern to both extracts. Results of the present study confirmed the potential use of seaweed extract as a source of antibacterial compounds.
  Nagi A. AL-Haj , Nurmas I. Mashan , Mariana N. Shamsudin , Habsah Mohamad , Charles S. Vairappan and Zamberi Sekawi
  Gram-positive bacteria are more sensitive to antibiotics than gram-negative bacilli because of the lack of outer membrane which prevent easy access of the drug into the bacterial cells. However, there are many gram-positive organisms with natural, intrinsic resistance to antimicrobials. In addition, these bacteria are able to acquire resistance to frequently used drugs rapidly through selective pressure of the environment and also via the genetic evolution of bacteria. The resistance of those bacteria to antibacterial agents is mediated by antibiotic resistant genes. Therefore, the current study was designed to explore the effect of seaweed extracts on several antibiotic resistant genes in Staphylococcus aureus mec genes mecA, mecR1 and mecI that regulate the expression of methicillin resistance was investigated by PCR.
  Nagi A. AL-Haj , Lai L. Suang , Mariana N. Shamsudin , Rasedee Abdullah , Rahmah Mohamed and Zamberi Sekawi
  Total of 23 putative Open Read Farms from B. pseudomallei strain D286 was successfully cloned and the nucleotide sequence analysis of the putative genes showed the homologue (98-100%) to strain K96243. The high similarity in gene sequences between these strains is confirmed for presence of the necessary ORF for LPS biosynthesis through PCR amplification the application of the ORFs in the PCR amplification and expression method. The findings of this study have contributed to some information on the molecular bases of the LPS biosynthesis genes in B. pseudomallei specifically for strain D286. PCR amplification, a specific pair of primer for each ORFs was proving specific for amplification of genes in B. pseudomallei strain D286. The PCR mixture with addition of DMSO, formamide and glycerol could ease the PCR optimization where different pairs of primers were involved. The specific primer pairs with the PCR mixture could be used in developing a PCR diagnosis of melioidosis.
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