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Articles by Zakir Hossain
Total Records ( 4 ) for Zakir Hossain
  Md. Fakruddin , Abhijit Chowdhury and Zakir Hossain
  PCR is the first method developed for amplification of DNA/nucleic acids in vitro. It was the method of choice since it's invent. PCR has many limitations which gave birth to alternative methods such as Loop Mediated Isothermal Amplification (LAMP), Nucleic Acid Sequence Based Amplification (NASBA), Self Sustained Sequence Replication (3SR), Rolling Circle Amplification (RCA) etc. A comprehensive review of the literature on the principles, limitations, challenges, improvements and alternatives of PCR was performed. Though PCR encountered some limitation afterwards and a no of technically improved alternatives has been devised worldwide, it still holds its standings as a gold standard for nucleic acid amplification preventing it from being obsolete. PCR has gone through some phenomenal modifications making continuous improvement of this method. Although, latter techniques offer better sensitivity and advantages where PCR has limitations, it is still commonly applied in the field of molecular biology for its extensive information all over the world, availability of reagents and methods, widespread validation and very good technical understanding among researchers. PCR is not obsolete despite of threats faced from other methods; rather it is continuously being modified to overcome the limitations. It will remain one of the ultimate methods of choice especially in developing countries for long.
  Tania Naznin , Mohammad Jakir Hossain , Tahmina Nasrin , Zakir Hossain and Mohammad Nasif Sarowar
  Background and Objective: Oomycetes (water molds) are ubiquitous organism thriving in moist or aquatic environment. They can cause devastating infections to animals and plants. Farmers, both crop and fish, count huge losses every year due to outbreak of oomycete infections. However, the lack of study in Bangladesh makes it harder to address the problem in the country. This study was conducted to investigate the diversity of oomycetes in the surrounding crop fields and water bodies of Bangladesh Agricultural University campus, Mymensingh, Bangladesh. Materials and Methods: The sampling took place during the winter season. A total of 356 water samples were collected out of which only seven came positive with oomycete growth. The seven isolates were grown in potato dextrose agar (PDA) plates. The isolates were identified using molecular methods that included DNA extraction, PCR amplification and subsequent sequencing of the internal transcribed spacer (ITS) region of the genomic DNA of the samples. Results: The BLAST analysis of the retrieved sequences to GenBank revealed that four of the isolates were Pythium catenulatum, two were Pythium rhizo-oryzae and the remaining isolate was Pythium torulosum strain. Pythium spp. are known to be plant pathogens of both crops and vegetables. Conclusion: The results suggest the evidence of plant pathogenic oomycetes around the study area that were causing damage to the crops and extend understanding of the diversity of the genus Pythium.
  Jianliang Xu , P. Jaya Kausalya , Dominic C. Y. Phua , Safiah Mohamed Ali , Zakir Hossain and Walter Hunziker
  ZO-1, ZO-2, and ZO-3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. Even though the zonula occludens (ZO) proteins are among the first TJ proteins to have been identified and have undergone extensive biochemical analysis, little is known about the physiological roles of individual ZO proteins in different tissues or during vertebrate development. Here, we show that ZO-3 knockout mice lack an obvious phenotype. In contrast, embryos deficient for ZO-2 die shortly after implantation due to an arrest in early gastrulation. ZO-2/ embryos show decreased proliferation at embryonic day 6.5 (E6.5) and increased apoptosis at E7.5 compared to wild-type embryos. The asymmetric distribution of prominin and E-cadherin to the apical and lateral plasma membrane domains, respectively, is maintained in cells of ZO-2/ embryos. However, the architecture of the apical junctional complex is altered, and paracellular permeability of a low-molecular-weight tracer is increased in ZO-2–/– embryos. Leaky TJs and, given the association of ZO-2 with connexins and several transcription factors, effects on gap junctions and gene expression, respectively, are likely causes for embryonic lethality. Thus, ZO-2 is required for mouse embryonic development, but ZO-3 is dispensable. This is to our knowledge the first report showing that an individual ZO protein plays a nonredundant and critical role in mammalian development.
  Zakir Hossain , Hideyuki Kurihara and Koretaro Takahashi
  Biochemical composition of brown alga Sargassum horneri was investigated by determination of moisture, protein, carbohydrate, ash, simple lipids and glycolipids. Fatty acid composition of simple lipids and glycolipids was determined by gas chromatography. The algal specimens were collected in two different months, January (sample-1) and February (sample-2). Moisture contents were 86.94 and 87.00% in sample-1 and sample-2, respectively. Protein, carbohydrate and ash contents were 22.94, 19.93 and 32.00% of the dry sample-1, respectively, while the contents of the dry sample-2 were 21.96, 20.81 and 33.58%, respectively. Glycolipids were 1.38 and 1.96% of the dry sample-1 and sample-2, respectively, whereas 2.45 and 2.75% when the samples were digested with the abalone Haliotis discus enzyme. Of the glycolipids obtained, MGDG, DGDG and SQDG were nearly 15, 15 and 68%, respectively. The major fatty acids in simple lipids were 16:0, 18:1 and 22:0. The major fatty acids in individual lipid class were 16:0, 18:1, 20:1, 20:4 and 20:5 in MGDG, 16:0, 20:0, 20:4 and 20:5 in DGDG and 16:0, 18:0, 18:1, 18:2 and 20:4 in SQDG. S. horneri is a potential source of valuable glycolipids.
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