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Articles by Zaiton Hassan
Total Records ( 2 ) for Zaiton Hassan
  Yousr Abdul Hadi , Zaiton Hassan , Gulam Rusul and Son Radu
  A total of 57 Listeria species strains (19 isolates of Listeria monocytogenes and 38 isolates of Listeria innocua ) isolated from different location in the state of Selangor (Seri Serdang, Seri Kembangan, Kajang and Bangi) were characterized by using RAPD-PCR fingerprinting technique. Primer GEN15002 and GEN15009 were chosen whereby it produced reproducible and typeable results in L. monocytogenes (19) and L. innocua (38) isolates examined with the bands sizes ranging from 0.25 to 3.0 kilobase pairs. From the current study, it can be concluded that the RAPD profiles revealed a wide variability and high level of DNA sequence diversity within Listeria species strains tested. RAPD showed a very discriminatory power since several RAPD profiles were obtained among L. monocytogenes and L. innocua isolates and even slight difference between isolates can be detected.
  Endang Purwati , Zaiton Hassan , Gulam Rusul , Raha Abdul Rahim and Son Radu
  The objective of present work was to optimize the procedures of FDA and USDA for the isolation of Listeria species in imported frozen beef samples marketed in Malaysia. The modifications consisted of direct analysis or storage of samples at 4°C for 24 h prior to analysis, and enrichment at 30°C or 35°C for 24, 48 and 168 h. For both FDA and USDA modified methods, storage at 4°C for 24 h and pre-enrichment at 24 and 48 h were the most efficient. However, the modified FDA with storage at 4°C for 24 h and pre-enrichment for 24 h (30°C and 35°C) and 48 h (30°C and 35°C) yielded more Listeria species. The rates of isolation were markedly affected with prolonged pre-enrichment incubation up to 168 h. The overall conclusion was that the modified USDA isolation method is beneficial when a limited range of the clinically important Listeria species is sought, whilst the modified FDA is needed to estimate the prevalence of Listeria species in the samples examined.
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