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Articles by Zahoor A. Swati
Total Records ( 3 ) for Zahoor A. Swati
  Nisar Ahmad , Iqbal Munir , Imtiaz A. Khan , Waqar Ali , Wisal Muhammad , Rakhshanda Habib , Raham Sher Khan and Zahoor A. Swati
  The most challenging hurdle facing Pakistan is the production of Brassica germplasm with a wider genetic base and using them properly in rapeseed genetic improvement. Genetic diversity was evaluated in 20 rapeseed lines (10 entries each of B. napus and B. campestris) using RAPD as molecular markers. Four Randomly Amplified Polymorphic DNA primers were used to estimate the genetic distances among the genotypes in all the possible combinations. The genetic diversity study revealed different levels of polymorphism for RAPD primers GLA07, GLB07, GLD18 and GLE07 that resulted in amplification of 3.2, 1.5, 3.0 and 3.5 scorable bands (loci) per genotype of Brassica napus and 2.5, 1.3, 2.6 and 3.7 scorable bands (loci) per genotype of B. campestris. Among Brassica napus genotypes, maximum genetic distance (79%) was observed between Torch+Maluka, Torch+Baro and Torch+Global, while, maximum genetic distance (91%) was observed between T-16 and P1-367601 genotypes of Brassica campestris. Individual genetic distance observed among the B. napus and B. campestris lines ranged from 21.50 to 59.41% and 53.75 to 60.09%, respectively. The dissimilarity coefficient matrix of these lines based on the data of four RAPD markers using UPGMA (Unweighted Pair Group of Arithmetic Means) method was also used to construct a dendrogram. The dendrogram analysis indicated that lines Torch and 366822 of B. napus, while 2163 and P1-392029 of B. campestris were genetically apart from other lines. These results provide valuable information for fingerprinting that can be used in a synergistic way to create wider genetic base and augment the breeding program of Brassica in Pakistan.
  Azhar Hussain Shah , Safdar Hussain Shah , Zahoor A. Swati and Zahid Hussain
  Ispaghol (Plantago ovata) husk was evaluated as a gelling agent for their performance in solidification of media for in vitro micropropagation/production of virus free potatoes (Solanum tuberosum L.). The objective of this study was to find a cheaper substitute for agar. After pilot study 12 gl-1 ispaghol was selected as suitable concentration for micropropagation. The data were recorded for growth parameters i.e. number of roots and number of nodes per plantlet, shoot length and relative growth rates. The results revealed that the performance of “Ispaghol gelled media” was excellently at par with agar solidified media. Cost of “Ispaghol” per plantlet was found to be 54 times lower than agar grown plantlets. It was concluded that ispaghol husk might emerge as a cheaper alternative for agar that could lead to substantial reduction in cost of production per potato plantlet.
  Zahoor A. Swati , Anwaar Ahmad , Zakirullah and Raziuddin
  Morphological differences among the Brassica lines for all the characters were statistically significant. Among the lines, AY produced maximum yield and was closely followed by WL and AAR with lower but statistically similar trend. As expected these lines were also statistically higher in number of pods per main stalk, pod size and thousand grain weight. Present efforts were quite successful in determining the genetic potential of different lines for yield and its components and are recommended for further testing and if proved stable could be released as an improved variety(s).
 
 
 
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