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Articles by Zahir Ali
Total Records ( 3 ) for Zahir Ali
  Gowher Ali , Fazal Hadi , Zahir Ali , Muhammad Tariq and Muhammad Ali Khan
  In the present study, regeneration conditions for two-cultivars-of-tobacco (Nicotiana tabacum L.) were optimized. At different concentrations the effects of 1-naphthalene acetic acid (auxin)-and-6-benzylaminopurine (cytokinin)-on-callus-induction-and-subsequent-plant regeneration in K-399 and SPTG-172 using Murashige and Skoog (MS) media were studied. The seeds were germinated on simple MS media, while MS media with auxine and cytokinin was used for callus induction and regeneration of shoots. In tobacco, callus was successfully induced from leaf explants using a high ratio of auxin: Cytokinin. The over all callus induction frequency in SPTG-172 was greater than K-399 indicating a difference in the genetic background of two cultivars. Both cultivars also showed best response to different hormonal concentrations. The resultant calli were stimulated to form shoots by increasing the cytokinin concentration and decreasing auxin content of culture media. There were clear differences between the two cultivars in their ability to produce regenerated plants. The maximum number of calli were regenerated in SPTG-172 whereas the mean number of shoots produced per callus at all concentrations was higher in K-399 indicating that’s shoot regeneration is markedly affected by the genotype. The roots developed well on hormones free MS media in both cultivars irrespectively of the varietal difference. The well-rooted plants were then transferred to green house for acclimatization-to-the in vitro environment.
  Alam Zeb , Zahir Ali , Taufiq Ahmad and Abdulloev Abdumanon
  Wheat grains a source of our daily food were obtained from the different ecological regions of Pakistan. These fifteen varieties namely Fakhr-e-Sarhad, Bakhtawar-92, Chudry-97, Gandam-2002, Wafaq-01, Bhakkar-01, Inqilab-91, Tatara, Ghaznawy, Saleem-2000, Watan, Oqab, Tuf, Zakht and Gandam-711 were evaluated for their physicochemical characteristics and minerals contents. Six of these varieties, Fakhr-e-Sarhad, Oqab, Tuf, Zakht, Watan and Inqilab-91 growing in the same ecological conditions in district Chitral were selected in order to show the effect of same ecological conditions on the overall productivity of wheat varieties in term of physicochemical and minerals contents. Results reveal that same variety growing in district Chitral shows good productivity than the rest of varieties growing in different ecological conditions.
  Mohd Shuaib , Ikhtiar Khan , Zahir Ali , Waqar Ali , Taufiq Ahmad and Alam Zeb
  Wheat seed-storage proteins represent not only an important source of food and energy but it is also involved in the determination of bread-making quality. Wheat grains of thirteen wheat varieties were collected from different ecological regions of Pakistan. The variability of seed storage-proteins was analyzed by using SDS-PAGE electrophoresis. Electrophorogram for each variety was scored and presence or absence of each band noted and entered in a binary data matrix. Based on electrophoresis band spectra, Jaccard‘s Similarity Index (JSI) was calculated. Genetic diversity of wheat was evaluated by constructing the dendrogram for High Molecular Weight (HMW) and Low Molecular Weight (LMW) gluten subunit bands. It is concluded that seed storage protein profiles could be useful markers in the studies of genetic diversity and classification of adapted cultivars, thereby improving the efficiency of wheat breeding programs in cultivar development especially in developing countries like Pakistan.
 
 
 
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