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Articles by Zafar Rasheed
Total Records ( 2 ) for Zafar Rasheed
  Zafar Rasheed , Rizwan Ahmad , Naila Rasheed , Trivendra Tripathi and Rashid Ali
  The study hypothesized that oxidative by-product like hydrogen peroxide (H2O2), would reacts with hemoglobin (Hb) and help to initiate autoimmunity in patients with type 1 diabetes. To test this hypothesis, Hb was modified by reactive oxygen species (ROS). Modification incurred in Hb was characterized by physico-chemical techniques. ROS-modified Hb was found to be highly immunogenic in rabbits as compare to native Hb. The binding characteristics of circulating autoantibodies in type 1 diabetes patients against native and ROS-modified Hb were assessed. Diabetes patients (n = 39) were examined by direct binding ELISA and their results were compared with healthy age-matched controls (n = 22). High degree of specific binding by 48% of patient`s sera towards ROS-modified Hb, in comparison to its native analogue (p<0.05) was observed. Competitive inhibition ELISA reiterates the direct binding results. Collectively, ROS caused extensive damage to Hb altering its immunogenicity, the neo-epitopes, thus generated might play a role in the induction of circulating autoantibodies in type 1 diabetes mellitus.
  Waleed Alabdulmonem , Homaidan T. Alhomaidan , Zafar Rasheed , Inamul Hasan Madar , Noura Alasmael , Shaza Alkhatib and Hussain Al Ssadh
  Background and Objective: Cluster of differentiation (CD) 74 is well known for its diverse proinflammatory and immunological functions. This study was undertaken to investigate the role of interferon (IFN)-γ on CD74 expression in breast cancer cells. Materials and Methods: The CAMA-1 and MDA-MB-231 cancer cells were stimulated with IFN-γ and expression of CD74 was determined by flow cytometry and western immunoblotting using Raji cells as a positive control. The MTT assays were used to determine the effect of IFN-γ on the proliferation and migration of cells. Whereas, cell surface expression of HLA-ABC and HLA-DR were acquired by flow cytometry. Results: Treatment of CAMA-1 and MDA-MB-231cells with IFN-γ significantly increased the expression of CD74. Not only have these, IFN-γ also stimulates the expression of HLA-ABC and HLA-DR in a dose-dependent manner in CAMA-1 and MDA-MB-231cells. Moreover, IFN-γ treatment also inhibited the proliferation and migration of CAMA-1 and MDA-MB-231cells. Conclusion: The IFN-γ up-regulates CD74 expression in human breast cancer cells. The results concluded that IFN-γ played a key role in modulating the expression of CD74, as well as in the proliferation and the migration of CAMA-1 and MDA-MB-231 cancer cells. Data suggest that CD74 plays a key role in tumor immunogenicity as well as in breast cancer immunoediting in humans.
 
 
 
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