Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by Zafar Rasheed
Total Records ( 2 ) for Zafar Rasheed
  Zafar Rasheed , Rizwan Ahmad , Naila Rasheed , Trivendra Tripathi and Rashid Ali
  The study hypothesized that oxidative by-product like hydrogen peroxide (H2O2), would reacts with hemoglobin (Hb) and help to initiate autoimmunity in patients with type 1 diabetes. To test this hypothesis, Hb was modified by reactive oxygen species (ROS). Modification incurred in Hb was characterized by physico-chemical techniques. ROS-modified Hb was found to be highly immunogenic in rabbits as compare to native Hb. The binding characteristics of circulating autoantibodies in type 1 diabetes patients against native and ROS-modified Hb were assessed. Diabetes patients (n = 39) were examined by direct binding ELISA and their results were compared with healthy age-matched controls (n = 22). High degree of specific binding by 48% of patient`s sera towards ROS-modified Hb, in comparison to its native analogue (p<0.05) was observed. Competitive inhibition ELISA reiterates the direct binding results. Collectively, ROS caused extensive damage to Hb altering its immunogenicity, the neo-epitopes, thus generated might play a role in the induction of circulating autoantibodies in type 1 diabetes mellitus.
  Waleed Alabdulmonem , Homaidan T. Alhomaidan , Zafar Rasheed , Inamul Hasan Madar , Noura Alasmael , Shaza Alkhatib and Hussain Al Ssadh
  Background and Objective: Cluster of differentiation (CD) 74 is well known for its diverse proinflammatory and immunological functions. This study was undertaken to investigate the role of interferon (IFN)-γ on CD74 expression in breast cancer cells. Materials and Methods: The CAMA-1 and MDA-MB-231 cancer cells were stimulated with IFN-γ and expression of CD74 was determined by flow cytometry and western immunoblotting using Raji cells as a positive control. The MTT assays were used to determine the effect of IFN-γ on the proliferation and migration of cells. Whereas, cell surface expression of HLA-ABC and HLA-DR were acquired by flow cytometry. Results: Treatment of CAMA-1 and MDA-MB-231cells with IFN-γ significantly increased the expression of CD74. Not only have these, IFN-γ also stimulates the expression of HLA-ABC and HLA-DR in a dose-dependent manner in CAMA-1 and MDA-MB-231cells. Moreover, IFN-γ treatment also inhibited the proliferation and migration of CAMA-1 and MDA-MB-231cells. Conclusion: The IFN-γ up-regulates CD74 expression in human breast cancer cells. The results concluded that IFN-γ played a key role in modulating the expression of CD74, as well as in the proliferation and the migration of CAMA-1 and MDA-MB-231 cancer cells. Data suggest that CD74 plays a key role in tumor immunogenicity as well as in breast cancer immunoediting in humans.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility