Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the
coverage of the most important and influential journals
to meet the needs of the global scientific community.
The enzyme βfructofuranosidase attacks beta-D-fructofuranoside (raffinose, stachyose and sucrose) from
the fructose end. Saccharomyces GCA-II was used for the production of βfructofuranosidase by
submerged fermentation. The effect of sucrose concentration and incubation time was worked out for
maximal enzyme production. Sucrose at the level of 30.0 g l-1 in fermentation medium was found to exert
an inducible effect on enzyme synthesis by Saccharomyces strain after 48 h of inoculation. Enzyme
production increased from 107.42 U ml-1 to 118.58 U ml-1 after optimization of the cultural conditions.
Specific product rate and specific growth rate were also determined for the production of βfructofuranosidase by Saccharomyces in shake flask.
