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A total of 400 hatchery samples comprised of yolk interior (100), paper pad (100), shell membrane (100) and fecal swab of newly hatched chicks (100) were tested to detect the presence of Salmonella organism by bacteriological agar plate test. Positive cases recorded in this study were 37 (37), 12 (12), 3 (3) and 19 (19%) from each sample (100) of yolk interior, paper pad, shell membrane and fecal swab of newly hatched chicks, respectively. A representative numbers of 50 isolates were used for the identification of serogoups of Salmonella prevailing in selected area by using polyvalent antisera. The result indicated that the test isolates 45(90%) were typed to a specific serogroup of "O". All 45 isolated Salmonella serogroup ‘O’ were then characterized by different specific biochemical media. Based on these tests, the selective isolates were identified as Salmonella gallinarum.
