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by
Z.J. Yin |
Total Records (
7 ) for
Z.J. Yin |
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Y. Liu
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X. Lu
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Y.R. Luo
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J.P. Zhou
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X.Y. Liu
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Q. Zhang
and
Z.J. Yin
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In this study, IRF1 gene was chosen as a candidate gene to evaluate its effect on porcine cytokine traits in serum. A SNP in exon2 was demonstrated by sequencing and PCR-RFLP analysis. Cytokine traits include IFN-γ and IL10 concentrations in serum were measured when the pigs were at 20 and 35 days of age, respectively. The further association analysis between SNP genotype and cytokine traits were conducted in three pig breeds including Large White, Landraces and Songliao Black pig, one Chinese indigenous breed. The results indicated that the SNP of IRF1 gene had highly significant effect on level of IFN-γ (day 20; day 35) in serum (p = 0.0001; p = 0.0001) and ratio of IFN-γ to IL10 (day 35) in serum (p = 0.0083). The study suggested that the IRF1 gene could be regarded as a molecular marker gene for genetic selection of cytokine traits in the further disease resistance breeding. |
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Y. Liu
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Y.R. Luo
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X. Lu
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X.T. Qiu
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W.X. Fu
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J.P. Zhou
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X.Y. Liu
,
Q. Zhang
and
Z.J. Yin
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In this study, haematological traits, lysozyme concentration and T lymphocyte subpopulation as markers of innate immunity were detected and compared among Landrace, Large White and a chinese indigenous breed, Songliao Black pig. The animals were of the same age and kept under the same environmental conditions to reduce non-genetic variation in immune traits; they were all apparently healthy and were vaccinated by CSF live vaccine at 21 days of age. Except LY%, MO%, CD4¯CD8¯% and RDW, the other detected immune traits were significant difference between before (20 days) and after vaccination (35 day). While the values of MCV, MCH, MCHC, MO%, CD4+CD8+% and CD4+CD8+/CD4¯CD8+ values decreased after vaccination, the others increased. The values of WBC, GR, LY, MO, CD4+CD8¯% and CD4+CD8+% were significant difference among Large White, Landrace and Songliao Black pig (p<0.05). Our analysis confirms that Songliao Black pig has better innate immune level than Landrace and Large White. The animal resource population was suggested to be appropriate to investigate further the QTL and genes contributing to differences on these innate immune traits in pigs. |
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L.M. Li
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X.L. Ding
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K. Qian
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Y.Y. Ding
and
Z.J. Yin
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In this study, we investigated the effect of microbes on the composting of pig manure. Geotrichum candidum, Yarrowia lipolytica, Bacillus coagulans, Bacillus stearothermophilus and Nocardia sp. were isolated from fresh pig feces. Fifteen different combinations of these microorganisms were added to a mixture of pig manure and sawdust for composting. An orthogonal, fractional factorial experimental design consisting of five factors and two levels was used. Statistical analysis indicated that Geotrichum candidum plus Bacillus stearothermophilus was the most effective combination. The results indicated that the addition of microbes to feces raised the maximum temperature of composting from 67-72°C and elevated the available nutrients by 9.5%. |
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Y.Y. Ding
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C.H. Zhang
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X.L. He
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L. Huang
and
Z.J. Yin
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The effects of Chinese Herbal Medicine Additives (CHMD) supplemented diets on weaned piglets growth performance, incidence of diarrhea, visceral organ mass, digesta pH, luminal microbial population and small intestinal morphology were studied in a 3 weeks trial. Total of 144 crossbred (Duroc x Landrace x Yorkshire) weaning piglets (BW = 5.86±0.24 kg) from 18 L with an age of 21 days were selected and divided randomly into 4 groups balanced for sex, weight and litter origin. In each group, the piglets were divided randomly into 3 pens (replicates, 12 pigs per pen), a corn-soybean meal-expanded soybean basal diet without antibiotics or probiotics was used as control and the other 3 groups were fed the control diet supplemented with the CHMD at rations of 0.5, 1 and 1.5% (wt/wt). In the 3 weeks trial, the data showed that. Compared to the control group, supplementation with 1% CHMD increased (p<0.01) Final Body Weight (FBW), Average Daily Weight Gain (ADWG) (p<0.01), Average Daily Feed Intake (ADFI) (p<0.05) and lower (p<0.01) F/G (total feed consumed (kg) by per kg gain in weight). Piglets fed the diet containing 1.5% CHMD had greater (p<0.05) ADWG, greater (p>0.05) ADFI, compared to the control group but without affecting F/G (p>0.05). There were no differences in ADFI, ADWG and F/G between the 0.5% CHMD group and the control group (p>0.05). Piglets fed the diet containing 1% CHMD and 1.5% CHMD both had lower (p<0.01) rate of diarrhoea, lower (p<0.01) incidence of diarrhoea and lower (p<0.01) index of diarrhoea compared to the control group. Piglets fed the diet containing 0.5% CHMD had lower (p<0.01) incidence of diarrhoea and lower (p>0.05) index of diarrhoea compared to the control group. Compared to the control group, piglets fed the diet containing 1% CHMD had higher (p<0.01) stomach weight and longer (p>0.05) small intestine, the 0.5% CHMD group and the 1.5% CHMD group both had no effect (p>0.05) on stomach weight and small intestine length. Compared to the control group, the 1% CHMD group and 1.5% both reduced the digesta pH of stomach (p>0.05), the 1% CHMD group reduced the digesta pH of duodenum (p>0.05), the 1% CHMD group and 1.5% both reduced the digesta pH of jejunum (p>0.05), the 1% CHMD group and 1.5% both reduced the digesta pH of ileum (p>0.05). Compared to the control group, the 1% CHMD group had a higher cecal and colonic lactobacilli count (p<0.01), a lower cecal and colonic Escherichia coli count (p>0.05) and had a higher LAB: E. coli ratio in the middle caecum (p<0.01), in the middle colon (p>0.05), pigs fed the diets with 1.5% CHMD had a higher cecal and colonic lactobacilli count (p>0.05) and had a higher LAB: E. coli ratio in the middle caecum (p>0.05). Compared to the control group, diets with 1% CHMD resulted in a greater VH in the duodenum (p<0.01), in the jejunum (p<0.01) and in the ileum (p>0.05), a lower CD in the duodenum (p<0.01), in the jejunum (p<0.01) and in the ileum (p>0.05), a greater (p<0.01) calculated VH:CD ratio in the duodenum in the jejunum and in the ileum, diets with 1.5% CHMD resulted in a greater (p>0.05) VH, a lower (p>0.05) CD and a greater VH:CD ratio (p>0.05) in the duodenum. The results show that the CHMD used in this study as a dietary additive could enhance indicators of gastrointestinal health, improve growth performance in weaned piglets, additionaly imply that the dose of 1% CHMD supplement is the most ideal concentration to achieve the most beneficial effects. |
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L.M. Li
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H. Diao
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X.L. Ding
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K. Qian
and
Z.J. Yin
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This study aimed at investigating the effect of Met or Lys as sole Nitrogen (N) and Carbon (C) sources on Glutamic Oxaloacetic Transaminase (GOT), Glutamic Pyruvic Transaminase (GPT) and Amino Acid (AA) metabolism of rumen microorganisms in vitro. Three fistulated goats were used as a source of ruminal microorganisms. Microbial suspensions with or without Met or Lys as substrate were anaerobically incubated at 39°C for 16 h. Free AA and transaminases in the supernatants of the incubation were analyzed by HPLC and an automatic biochemistry analyzer, respectively. When Met was used as a unique source of N and C, the content of free Val and His were significantly (p<0.01 and p<0.05) reduced but Gly was increased (p<0.01). No significant differences in the other free AA were found as well as for GOT and GPT activities. When Lys was used as a sole source of N and C, the content of free His and activities of GOT and GPT were significantly lower (p<0.01) than that of controls however Tyr was significantly increased (p<0.05). The results indicated that Val and Cys are probably the most important AA of rumen microorganisms when Met serves as the only source of N and C. But when Lys serves as a sole source of N and C, His becomes the most important AA. At the same time, GPT plays a weaker role when Met serves as a sole source of N and C. |
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Y.Y. Ding
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C.H. Zhang
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X.L. He
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L. Huang
and
Z.J. Yin
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The study investigated the effects of Chinese Herbal Medicine Additives (CHMD) supplemented diets on weaned piglets antioxidant status, serum biochemical parameters, digesive enzymatic activities in a 3 weeks trial. Total of 144 crossbred (Duroc x Landrace x Yorkshire) weaning piglets (BW = 5.86±0.24 kg) from 18 L with an age of 21 days were selected and divided randomly into 4 groups balanced for sex, weight and litter origin. In each group, the piglets were divided randomly into 3 pens (replicates, 12 pigs per pen), a corn-soybean meal-expanded soybean basal diet without antibiotics or probiotics was used as control and the other 3 groups were fed the control diet supplemented with the CHMD at rations of 0.5, 1 and 1.5% (wt/wt). After completion of the feeding experiment, 3 piglets from each treatment were randomly selected to determine the antioxidant status, serum biochemical parameters, digesive enzymatic activities. In the 3 weeks trial, the data showed that the 1% CHMD group had higher protease activity in stomach digesta than the control and 0.5% CHMD group (p<0.01) than 1.5% CHMD group (p<0.05). The 1.5% CHMD group showed similar significant difference in protease activities of piglets compared with the control group (p<0.05). The 1% CHMD group had higher lipase activity than the control group in the stomach and jejunum digesta (p<0.01) in the duodenum and ileum digesta (p<0.05) and than the 0.5% CHMD group in the stomach and jejunum digesta (p<0.05). The 1% CHMD group had higher amylase activity than the control group in the jejunum digesta (p<0.01) in the duodenum and ileum digesta (p<0.05) and than the 0.5% CHMD group and 1.5% CHMD group in the jejunum digesta (p<0.05). The 1.5% CHMD group had higher amylase activity than the control group in the ileum digesta (p<0.05). The 1% CHMD group had higher T-AOC and CAT activities than the control group and 0.5% CHMD group (p<0.01), than the 1.5% CHMD group (p<0.05). The 1.5% CHMD group had higher T-AOC and CAT activities than the control group (p<0.05). The 1% CHMD group had higher GSH-PX and SOD activities than the control group (p<0.05). The 1% CHMD group (p<0.01) and the 1.5% CHMD group (p<0.05) had lower MDA content than the control group. Compared with the control group, dietary supplementation of 1% CHMD reduced the serum BUN (p<0.05) and increased the total serum protein (p<0.05), reduced the serum triglyceride and total cholesterol contents (p<0.01). The results show that the CHMD used in this study as a dietary additive could enhance the antioxidant status, serum biochemical parameters, digesive enzymatic activities in weaned piglets, additionaly imply that the dose of 1% CHMD supplement is the most ideal concentration to achieve the most beneficial effects. |
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X.C. Cao
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D.L. Xu
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H.M. Guo
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C.J. Liu
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Z.J. Yin
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X.H. Li
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K. Qiu
and
Y.Q. Wang
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Influence of reactor pressure on the quality of GaN layers grown by hydride vapor phase epitaxy (HVPE) has been studied. With the reactor pressure decreasing from 7 to 5 × 104 Pa, improvements in structural, optical, and electrical properties of the GaN films have been observed. An investigation of the surface morphology of the GaN films reveals that the improvements arise from the change of the growth mode from an island-like mode at high pressures to a step-flow one at low pressures. These results clearly indicate that the reactor pressure, similar to the growth temperature, is one of the important parameters to control the qualities of HVPE-GaN epilayers. |
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