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Commercial laying hens (Babcock) 174-weeks-old, were given feed containing 1, 2 and 3% EM4 for a period of 12 weeks. EM4 did not influence live body weight. Egg production was greater in birds given feed containing 1 and 2% EM4 but lower in birds given feed containing 3% EM4 than the control birds. Blood glucose was significantly lesser in birds given feed containing 3% EM4 than the control (p<0.05). Serum phosphorus was significantly lesser in birds given feed containing 3% EM4 than the control birds (p<0.01). Serum total proteins, serum albumin, serum globulins, serum total lipids and serum cholesterol were not influenced significantly with the EM4 treatment. The study suggests that EM4 is a safe product for laying birds it increases egg production when mixed in feed.
