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Silene L. from Caryophyllaceae, comprises about 92 species in Iran which 16 species have been recorded from North-East of Iran. In present research, seven Silene species and four subspecies from four sections from Khorassan Razavi Province in North-East of Iran identified using floral segments morphology e.g. shape of calyx’s denth, margin of calyx’s denth, paracorolla shape and micromorphological characters like seed coat and pollen ornamentation. The results demonstrated using above mentioned characters are very useful for exact identification of Silene species.