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Articles by Z. Ishak
Total Records ( 3 ) for Z. Ishak
  N. ALHaj , N.S. Mariana , A.R. Raha and Z. Ishak
  A PCR for detection of two categories of diarrheagenic Escherichia coli was developed. This method proved to be specific and rapid in detecting virulence genes from enteropathogenic E. coli (EPEC) (eae and bfp) and Shiga toxin-producing E. coli (STEC) (stx1, stx2 and eae) from seventy isolates of various sources. Present results confirm that it is possible and feasible to perform a simultaneous amplification of the virulence genes from two categories of diarrheagenic E. coli (STEC, EPEC) and that this technique becoming a novel diagnostic tool for future water food-borne outbreaks studies.
  N. ALHaj , N.S. Mariana , A.R. Raha and Z. Ishak
  Diarrhea caused by multidrug-resistant bacteria is an important public health problem among children in developing countries. Escherichia coli is an important cause of disease in animals and humans worldwide. Twenty five E. coli isolates with rate 61.2% among human and environments were tested for susceptibility to 10 antimicrobial agents by disk diffusion method. Resistant isolates were screened by molecular methods for resistance genes, TetA, TetB, strepA, MarI and MarII. Molecular result showed that all isolates harbored resistance gene for the TetA, TetB, strepA, MarI, and MarII even though the genotypic test showed sensitive to the drugs. E. coli isolates exhibit a wide repertoire of genetic elements to sustain antimicrobial pressure. The results of this study using pheno-genotypic techniques highlight the distribution of E. coli among human, animal, aquatic ecosystems and the potential public health threat of E. coli originating from municipal wastewater sources.
  N. ALHaj , N.S. Mariana , A.R. Raha and Z. Ishak
  Antimicrobial agent resistance has been recognized as an emergingworldwide problem in both human and animals, antimicrobial agent use is considered the most important factorfor the emergence, selection and dissemination of antimicrobialagent-resistant bacteria, intrinsically either acquires the resistance gene from other bacterial environment or development of pumping out mechanism. The aim of this study was to generate baseline dataon the prevalence of antimicrobial resistancein Escherichia coli isolates from different sources. Seventy E. coliisolates from humans and environments were tested for susceptibility to 10 antimicrobialagents by diffusion method. Resistance was found in 61.2 % of the isolates. The most prevalent resistanceswere to kanamycin and tetracycline (81.4%),followed by chloramphenicol (75.7%) and gentamicin,(74.3%). The low prevalent were to cefetoxin (44.3%), norofluoxacin (27.1%) and ciprofluoxacin (24.3%). This study showed the distribution of antimicrobial agent resistancein E. coli isolates from a variety of sources and analysisof such patterns of resistance may prove to be useful beyondsimple description. Regarding to the concern of water quality and environmentalcontamination by human and agricultural waster have increased,it has become increasingly important to develop low-cost screeningtools that can be used to identify the most probable sourceof contamination.
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