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Articles by Yusuf Bektas
Total Records ( 3 ) for Yusuf Bektas
  Yusuf Bektas and Ali Osman Belduz
  Morphological variation among Turkish populations of the Atlantic horse mackerel Trachurus trachurus (Carangidae) was examined for 150 specimens at five different sampling localities (Sea of Marmara (Istanbul and Canakkale), Aegean (Izmir) and Northeastern mediterranean (Antalya and Iskenderun) seas) in Turkey by discriminant function analysis of eleven morphometric and five meristic characters. The results of discriminant function analysis showed that there is limited gene flow among T. trachurus populations in Turkish coastal waters. The Marmara Sea sample was similar to the Aegean Sea samples for both morphometric and meristic data. Morphometric and meristic data showed the heterogeneity between Aegean and Mediterranean mackerel samples. The Mediterranean samples separated from all others and indicate 2 local populations (Iskenderun and Antalya) for morphometric data, unlike meristic data that revealed no significant differentiation between 2 Mediterranean localities. Discriminant function analyses indicated that morphometric differentiation between the samples was largely due to differences in the head characters of fish. It is confirmed that metric characters are much less effective for discriminating between T. trachurus populations than morphometric characters, which is expressed higher distances between samples. The results indicate a small degree of spatial separation in morphology in T. trachurus among the studied localities.
  Yusuf Bektas and Ali Osman Belduz
  PCR based nuclear and mitochondrial DNA markers are used to clarify cases of misidentification of adult individuals of fish species with morphological similarities. This problem prevails in the case of false scad (Caranx rhonchus) overlap geographically and has similar morphology with Turkish species of the genus Trachurus. Hence, the nuclear DNA/mitochondrial DNA approach was used for C. rhonchus, to identify and discriminate, it from Trachurus sp. coexist. Polymerase Chain Reaction (PCR) amplification of the nuclear 5S rDNA gene is a suitable method for differentiating between C. rhonchus and Trachurus sp. On the other hand, a PCR-RFLP technique, which involves digesting of the whole mitochondrial cytochrome b gene with XcmI and BsrGI restriction enzymes to generate DNA profiles, was used to identify of C. rhonchus and discriminate it from three Trachurus sp. This PCR-RFLP analysis is simple, rapid and reliable and hence can be regularly used to discover fraudulent substitution among economically important C. rhonchus and Trachurus sp. Results have confirmed the usefulness of these techniques to distinguish and genetically identification of morphologically similar carangid species.
  Yusuf Bektas and Ali Osman Belduz
  The Random Amplified Polymorphic DNA (RAPD) technique offers an useful tool to investigate DNA polymorphisms. It can be used to distinguish different populations belong to one species. These markers also represent an efficient and inexpensive way to generate molecular data and thus, they have been used successfully in various taxonomic and phylogenetic studies. Information on the genetic structure of native fish populations is essential for studying molecular systematics and optimising fisheries management. RAPD assay was evaluated for studying genetic relationships and diversed in eight populations of whiting (Family: Gadidae). We used RAPD to determine the genetic characterization and the stock differentiation of whiting, Merlangius merlangus euxinus, eigth populations in the Black Sea coast of Turkey by using eleven arbitrary primers. The genetic relationship among the determined eight stations was estimated according to Jaccard similarity index and cluster analysis. Jaccard similarity coefficient values ranged from 0.676 to 0.836. The lowest similarity (0.676) was found between Karasu ve Rize and the highest similarity (0.836) was between K y koy ve Zonguldak. In consequence of cluster analysis, two stations were classified in the first branch of the derived dendogram while the others were classified in the second branch. The average similarity between the two branches was 0.720. Among the primers tested, OPAB-01, 08, 14, 17, OPA-08, 12, 19, OPB-08 and OPC-11 showed polymorphic bands. Amplified fragments ranged from 218 to 2196 base pairs and the numbers of bands for each primer varied from 2 to 9.
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