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Articles by Yurnalis
Total Records ( 2 ) for Yurnalis
  Yurnalis , Husmaini and Sabrina
  Objective: The objective of this study was to determine Growth Hormone (GH) gene polymorphisms and their association with productive traits, including body weight, at ages 1, 2, 3, 4, 5, 6, 7 and 8 weeks. Methodology: Polymorphisms in exon 1 of the GH gene were evaluated in two duck populations in West Sumatra Province Indonesia (Pitalah and Kumbang Janti ducks). For this purpose, blood samples were collected and DNA samples were extracted using the Promega Wizard® Genomic DNA Purification Kit. For this purpose, a total 225 ducks blood samples were collected from 145 male and 80 female ducks. Genetic polymorphisms were determined with the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the Eco721 restriction enzyme and agarose gel electrophoresis. Direct sequencing of some samples was used to confirm the results. Results: Two alleles (GHG and GHA) and three genotypes (GH/GG, GH/GA and GH/AA) were found in the studied duck samples at locus GH/Eco721. In both groups of ducks, the dominant allele was GHG. The most frequent genotype in the examined ducks was GH/GA. Three genotypes were observed in the Pitalah ducks, whereas two genotypes (GH/GA and GH/GG) were identified in the Kumbang Janti ducks and in the males. Pitalah ducks with the GH/GA genotype were characterized by a higher (p<0.01) body weight than the ducks with the GH/GG and GH/AA genotypes. This same trend was observed in the female Pitalah ducks; individuals with the GH/GA genotype had higher body weights (p<0.05 and p<0.01) than the birds with the two other detected genotypes. Kumbang Janti ducks with the GH/TT genotype were distinguished by higher values of all evaluated traits compared to the ducks with the GH/CT and GH/CC genotypes; however, most of the recorded differences were not significant. The only trait that was markedly impacted (p<0.05) by the polymorphism of GH gene intron 1 was the body weight at 5, 6, 7 and 8 weeks. Conclusion: This study found that the GH/TT genotype was associated with a higher body weight at 5, 6, 7 and 8 weeks of age in Pitalah and Kumbang Janti ducks.
  Yurnalis , Arnim and Dino Eka Putra
  Background and Objective: The insulin-like growth factor 1 gene (IGF1) is a candidate gene for marker-assisted selection strategies. Two single nucleotide polymorphism of IGF1 gene namely IGF1/SnaBI and IGF1/TasI has been reported to be associated with production traits in several cattle breeds. One single nucleotide polymorphism in exon 4 namely IGF1/Rsa1 has a significant influence (p<0.01) on birth weight, weaning weight and average daily gain of Bali cattle. The objective of this study was to determine the IGF1/SnaBI, IGF1/TasI and IGF1/RsaI polymorphisms and their associations with average daily gain in Pesisir cattle local breed from west Sumatera Indonesia. Methodology: The isolation of DNA from 183 blood sample was performed using DNA extraction kit (Promega DNA purification kit) following the manufacture procedure. The amplification of IGF1 fragment was done by master mix from Thermo Scientific. Results: In the case of the IGF1/SnaBI polymorphism, three genotypes were observed, frequencies were 0.011, 0.011 and 0.978 for AA, AB and BB, respectively. This gives frequencies of 0.064 and 0.9836 for A and B alleles. For IGF1-TasI locus showed that AA genotype occurred with a frequency of 0.978, AC with 0.011 and CC with 0.011 with frequency for allele A and C were 0.9836 and 0.0164, respectively. In the case IGF1/RsaI polymorphism produces only TT and CT genotype with frequency 0.027 and 0.973 respectively and frequency of T and C allele were 0.5137 and 0.4863, respectively. Conclusion: Among these 3 polymorphism only IGF1/RsaI was associated with average daily gain, Thus this polymorphism could be a potential for improving body weight in Pesisir cattle. Association study for IGF1/SnaBI and IGF1/TasI were strongly limited by low frequency of one allele.
 
 
 
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