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Articles by Yun Zhang
Total Records ( 5 ) for Yun Zhang
  Patrick Duriez , Yun Zhang , Zexun Lu , Andrew Scott and Edward Topp
  Confined livestock production farms typically store their wastes prior to land application. Here, we employed three complementary approaches to evaluate changes in the population structure and stability of virulence genes in Escherichia coli during manure storage on a commercial farm that housed healthy swine. Isolates were genotyped by repetitive extragenic palindromic PCR using the BOXA1R primer and evaluated for the presence of selected virulence genes by PCR. Isolates obtained from the manure holding tank (n = 392) carried estB, fedA, stx2e, astA, paa, aida-I, and sepA at lower frequencies than isolates obtained from fresh feces (n = 412). Fresh fecal material from the barn was added into diffusion chambers and immersed in the manure holding tank for 7 weeks. The fecal E. coli population was initially dominated by a single genotype, all isolates of which carried fedA and aida-I. After 7 weeks, a genotype that did not carry any virulence genes dominated the surviving population. In a second experiment, 48 fecal isolates of E. coli that varied in their genotypes and virulence gene complement were incubated in diffusion chambers in the manure holding tank for 3 weeks. Over 95% of the inoculum population carried at least one virulence gene, whereas after 3 weeks 90% of the recovered isolates carried no virulence genes. Taken together, these results indicate that during commercial manure storage, there was a significant reduction in the carriage of these virulence genes by E. coli. We propose that loss of virulence genes from enteric pathogens in the farm and in natural environments may, if generalized, contribute to the attenuation of a public health risk from contamination with agricultural wastes.
  Yun Zhang , Zhiyong Li , Yongzhu Yi , Xiangping Yin , Biao Yang , Zhifang Zhang , Wenqiang Jiao and Jixing Liu
  The structural protein VP1 of foot-and-mouth disease virus (FMDV) play an important role in the construction of a high immunogenic subunit vaccine. The objective of this study was to obtain higher expression of type-O FMDV target protein, this will provide further experimental evidence through animal immunization. The VP1 which in Bombyx mori Baculovirus recombinant transfer vector pVL-P12A3C of Asia I FMDV was replaced by VP1 of type-O FMDV and the recombinant plasmid pVL-OP12A3C was created. The Bombyx mori N (Bm-N) cells was co-transfected with pVL-OP12A3C and linearized DNA of insect virus expression vector. Indirect immune fluorescence tests (IFAT) demonstrated that target proteins were expressed. The recombinant virus was used to infect silkworm and the target protein in haemolymph was characterized by western blotting and double-antibody sandwich Enzyme-linked Immune Sorbent Assay (ELISA) analysis. The results indicated that gene replacement is an effective way to enhance gene expression.
  Hongxing Guan , Zhiyong Li , Xiangping Yin , Yun Zhang , Peng Gao , Yinmei Bai and Jixing Liu
  Aiming at providing an effective method for rapid detection of Foot-and-Mouth Disease Virus (FMDV), this study developed an antigen-capture reverse transcriptase loop-mediated isothermal amplification method (Ag-RT/LAMP). Diluted FMDV were bond by the tubes coated with IgG of FMDV and then detected by Loop-mediated isothermal amplification (LAMP). This method could detect as few as 0.58x102 copies of virus and showed higher sensitivity than RT-PCR and could also differentiate FMDV serotypes with high sensitivity and specificity, but if the samples contain high concentrations of virus this serotype specificity may be unstable. In addition, the entire reaction required only a single incubation at 63°C for 3 h. The amplification products could be visually inspected for color change. Ag-RT/LAMP is sensitive, serotype specific, cost-effective, time-saving, and versatile, with the potential for analysis of field clinical specimens in developing countries for FMDV surveillance.
  Gang Cao , Qiyuan Shan , Chengrong Zhang , Yun Zhang , Hao Cai , Xiaodong Cong and Baochang Cai
  Context: Fructus Corni is derived from the dry ripe sarcocarp of Cornus officinalis Sieb. et Zucc. (Cornaceae). Morroniside is an active constituent of Fructus Corni used in many traditional Chinese medicines (TCMs). This article describes a sensitive and specific assay for the quantitation of morroniside in rat plasma after oral administration of iridoid glycosides from Fructus Corni. Materials and methods: In this article, back-propagation (BP) neural network method was fist developed for the prediction of pharmacokinetic (PK) parameters of morroniside in Fructus Corni. Results: The results show that mean square error (MSE) of neural network model with 11 hidden neurons and 90% training data is 0.092. Discussion and conclusion: This article provides a new method to calculate PK data, one do not need to figure out all the compartment parameters to acquire PK data of morroniside. Therefore, the BP neural network method would be useful for guiding the holistic PK study in consistence with the intrinsic theory and characteristics of TCM.
  Ming-Xiang Zhang , Cheng Zhang , Ying H. Shen , Jian Wang , Xiao Nan Li , Yun Zhang , Joseph Coselli and Xing Li Wang
  Endothelial nitric-oxide synthase (eNOS) is a constitutively expressed gene in endothelium that produces NO and is critical for vascular integrity. Previously, we reported that the 27-nucleotide (nt) repeat polymorphism in eNOS intron 4, a source of 27-nt small RNA, which inhibits eNOS expression, were associated with cardiovascular risk and expression of the eNOS gene. In the current study, we investigated the biogenesis of the intron 4-derived 27-nt small RNA. Using Northern blot, we showed that the eNOS-derived 27-nt short intronic repeat RNA (sir-RNA) expressed only in the eNOS expressing endothelial cells. Cells containing 10 x 27- or 5 x 27-nt repeats produced higher levels of 27nt sir-RNA and lower levels of eNOS mRNA than the cells with 4 x 27-nt repeats. The 27nt sir-RNA was mostly present within the endothelial nuclei. When the splicing junctions of the 27-nt repeat containing intron 4 in the full-length eNOS cDNA vector were mutated, 27nt sir-RNA biogenesis was abolished. Suppression of Drosha or Dicer diminished the biogenesis of the 27nt sir-RNA. Our study suggests that the 27nt sir-RNA derived through eNOS pre-mRNA splicing may represent a new class of small RNA. The more eNOS is transcribed or higher number of the 27-nt repeats, the more 27nt sir-RNA is produced, which functions as a negative feedback self-regulator by specifically inhibiting the host gene eNOS expression. This novel molecular model may be responsible for quantitative differences between individuals carrying different numbers of the polymorphic repeats hence the cardiovascular risk.
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