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Articles by Yuki Okada
Total Records ( 4 ) for Yuki Okada
  Nobuko Mori , Hiroshi Takemitsu , Yuki Okada , Ichiro Yamamoto and Toshiro Arai
  Many veterinarians feel that obese dogs are increasing in prevalence worldwide. However, no data are available; thus, it is difficult to distinguish overweight from obesity as a sign of metabolic syndrome. The objectives are (1) To reveal the ratio of overweight and obese house dogs in Japan; and (2) To investigate differences in breed, gender, body condition and age of various purebreds on healthy dogs. Twelve metabolic parameters were assayed in 888 healthy dogs. The data were analyzed from various viewpoints of age, gender, Body Condition Score (BCS), castration and spay and breeds. The ratio of obese dogs was approximately 27% of all dogs examined. When the effects of aging were assessed, older females were found to be more affected by aging than males. In particular, lipid metabolism parameters, such as triglycerides (TG), Total Cholesterol (T-cho) and Free Fatty Acids (FFA) as well as insulin concentrations in female dogs >11 years old were the highest of all age groups. Because T-cho and insulin concentrations in BCS4 dogs were significantly high, these parameters could be useful as obesity markers in healthy dogs. Creatinine concentrations in small breed dogs, such as Beagles, Cavaliers, Chihuahuas, Miniature dachshunds, Pomeranians, Shih tzus and Yorkshire terriers, were lower than those in middle-sized and large-sized dogs. TG and FFA levels in neutered dogs were significantly higher than those in unaltered dogs. In future, it will be important to measure and assess metabolic parameters in healthy dogs to identify differences among different regions.
  Gebin Li , Peter Lee , Yuki Okada , Ichiro Yamamoto , Toshiro Arai and Nobuko Mori
  The objective of this study was to determine whether Peripheral Blood Leukocytes (PBL) are sensitive enough to detect early signs of diet induced obesity related changes occurring in insulin sensitive tissues, such as abdominal omental and subcutaneous adipose, liver and skeletal muscle, by comparing transcriptome profiles of insulin signaling (IRS-1, IRS-2 and PI3-K p85 α), adiponectin signaling (AdipoR1 and AdipoR2), energy homoeostasis (G6PDH and MDH) and sterol metabolism (FASN) genes as determined by RT-PCR in cats fed on High Fat (HF) diet. Regarding PBL concordance, using a HF diet induced obesity cat model, out of seven genes examined, concordance was observed with ~60% (5 out of 8) of them (IRS-1, IRS-2, Adipo-R1, Adipo-R2 and MDH) between PBL and tissue transcriptomes. HF diet cat PBL IRS-1 and IRS-2 mRNA expression were both reduced, when compared to control diet which was in concordance with reduced IRS-1 and IRS-2 mRNA expression in both abdominal and subcutaneous adipose of HF diet cats. Similar to IRS-1 and IRS-2, AdipoR1 and AdipoR2 mRNA expression in HF diet cats was also reduced, when compared to control diet which was in concordance with reduced AdipoR1 and AdipoR2 in liver and skeletal muscle, respectively of HF diet cats. Lastly, PBL MDH mRNA expression was reduced and was concordant with reduced mRNA expression in liver and skeletal muscle. Overall, our results demonstrate that PBL are sufficiently sensitive to high fat diet induced alterations to transcriptomes of insulin sensitive tissues and can serve as surrogate tissue for various insulin sensitive tissues.
  Koh Kawasumi , Yuki Okada , Nanae Kashiwado , Eiji Iwazaki , Nobuko Mori , Ichiro Yamamoto and Toshiro Arai
  The efficiency of licorice flavonoid supplement as anti-obesity substance was investigated in 14 client-owned dogs. This flavonoid supplement reduced whole and visceral fat in overweight humans. All experimental dogs were given the supplement at a dose of 10 mg kg-1 day-1 in the evening with food for 8 weeks. After 8 weeks post-administration, the body weight was decreased in 11 animals (78.6%). The decreasing rate was 2.51±0.6% (Mean±SE). Serum glucose (GLU), Triglyceride (TG), Malondialdehyde (MDA) concentrations and aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Glutathione peroxidase (GSHPx) activities were decreased but the changes were not statistically significant. On the contrary, serum adiponectin (ADN) concentrations, though statically significance was not seen, tended to increase after 8 weeks post-administration of the supplement compared to those in baseline. In conclusion, the licorice flavonoid supplement was suggested to show anti-obesity effect accompanied with improvement of lipid metabolism in tissues of dogs.
  Makoto Habara , Makoto Tamanuki , Shingo Ishikawa , Hiroshi Takemitsu , Nobuko Mori , Yuki Okada , Nobuhiro Nakao , Koh Kawasumi , Katsumi Ishioka , Toshiro Arai and Ichiro Yamamoto
  G-protein-coupled receptors (GPRs) 40 and 120 are members of the Free Fatty Acid (FFA) receptor group and are termed FFAR1 and FFAR4, respectively. The aim of this study was to clone cat GPR40 and GPR120 cDNAs in several tissues. There was high sequence homology to other mammalian GPR40 and GPR120, with encoding 320 and 361 amino acid residues, respectively. Cat GPR40 encoded extra 21 amino acid residues in the C-terminal cytoplasmic region. Quantitative RT-PCR revealed expression of GPR40 mRNA in the duodenum, liver and pancreas. The GPR120 mRNA was expressed in adipose tissues, cerebral cortex and colon. In conclusion, GPR40 and GPR120 were well conserved and were expressed in cat tissues with different distribution patterns.
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