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Articles by Yujian Sui
Total Records ( 2 ) for Yujian Sui
  Yongfeng Sun , Tongao Yang , Jingtao Hu , Zhe Hao , Yujian Sui , Yingying Fu , Lu Chen , Haiyang Zhu and Wei Wu
  The muscular tissue of breast was dissected from 8 weeks old Jilin White goose in the present study. The big fragment PCR Method was used to amplify double-strand cDNA based on the SMART techniques for construction of a full-length cDNA library. After digestion with restriction endonuclease Sfi |, a modified vector of pBluescript II SK-plasmid with the adaptors containing Sfi |A and Sfi |B sites was used to recombine with the cDNA products amplified. The recombinants were cloned by transformation into competent Escherichia coli DH2α. A plasmid cDNA library with goose muscle was constructed. The results showed that the titer of the cDNA library was 1.01x106 pfu mL-1 and the percentage of recombinant clones was 97%. The length of most cDNA inserted was between 0.25 and 1.6 kb identified by gel electrophoresis after cDNA PCR amplification. The unigene ratio was 66.7% and the percentage of complete cDNA sequences was 80% by estimating from the 24 clones sequenced randomly. It is helpful to study muscle development of goose at molecular level in the future.
  Yongfeng Sun , Zhe Hao , Yujian Sui , Lifeng Zhao and Guang Gao
  In this study, researchers detected the expression of IGF-I mRNA at 1 (day 1), 30 (day 30), 60 (day 60th) and 90 (day 90th) days of age in the breast muscle and leg muscle of geese from Jilin White goose (meat type, egg type) and Hortobagyi goose. Researchers found that the expression of IGF-I mRNA in skeletal muscle tissues differed based on developmental age. The expression of IGF-I mRNA exhibited a trend that ascended firstly and then descended after birth. IGF-I mRNA was expressed in all breast muscle tissues during each stage with higher levels in meat type goose and Hortobagyi goose than that in Jilin egg type goose (p<0.05). The expression pattern of IGF-I mRNA in breast muscle of meat type goose was similar with this in and Hortobagyi goose and in leg muscle, IGF-I mRNA expression patterns were all down-regulated from day 30. Similarity in IGF-I mRNA expression pattern were found in breast among Jilin meat type goose, egg type goose and Hortobagyi goose which indicates that IGF-I may be related to skeletal muscle differentiation and growth and suggests IGF-I mRNA maybe tissue-specific expression and individual difference.
 
 
 
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