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Articles by Yuherman
Total Records ( 8 ) for Yuherman
  Yuherman , Son Radu , Gulam Rusul , Lum Keang Yeang , Ooi Wai Ling and Jamal Khair
  DNA fingerprinting by PCR amplification of enterobacterial repetitive intergenic consensus (ERIC) and pulsed-field gel electrophoresis (PFGE) were used to compare environmental and clinical isolates of Vibrio cholerae O1 and non-O1. All the V. cholerae O1 and non-O1 isolates were typable using ERIC PCR. Though PFGE generated banding patterns to discriminate the isolates into twelve fingerprints, eight isolates were untypable by PFGE due to consistent degradation of the bacterial DNA. Based on the dendrogram generated from ERIC-PCR method, three of the clinical isolates (C1, C2 and C3) were closely related to environmental isolates (E6 or E10). The results indicate that ERIC-PCR is a very discriminative and efficient method for studying genetic diversity of V. cholerae isolates.
  Yuherman , Reswati , Yulianti Fitri Kurnia , Indahwati and Khalil
  Background and Objective: Female exotic breed cattle raised by traditional small farms are susceptible to reproductive disturbances that result in failure or delay to produce calves. The present research investigated the hematologic profiles of exotic breed cattle having reproductive troubles versus heifers and pregnant cows raised by small farms. Materials and Methods: A survey was conducted to identify the reproduction performance of 160 female Simmental cows raised at 15 smallholders in Payakumbuh, West Sumatra, Indonesia. Samples of blood were collected from 15 female Simmentals comprised of three different reproductive statues (heifers, pregnant and reproductive failure, n = 5 animals each). Blood plasma was then separated and analyzed for hematological parameters [hemoglobin (Hb), hematocrit, red and white blood cell, mean corpuscular Hb concentration], total protein and mineral content (Ca, P, Mg, Fe, Mn, Cu and Zn). Samples of fodder feed were collected from 15 farms for determination of dominant species and mineral composition. Data were statistically analyzed in a completely randomized 3×5 design for blood parameters and 4×3 design for forage minerals. Results: About one-third of female cows found to have reproductive problems. Anestrus was found to be the most important causal factor for reproductive failure, followed by postpartum infertility (poor fertilization). The reproductive failure group had significantly lower (p<0.05) Hb levels, hematocrit, red and white blood cell and protein but higher mean corpuscular Hb concentration. Considering the critical levels in the blood and feed, the animals were deficient in protein, Ca, P, Mn and Cu. Conclusion: Anestrus and repeated insemination were found to be the major causes of reproductive disorders in exotic breed cattle under small farm conditions that results in delay or failure to produce calves. Reproductive disturbances in Simmental cows were most likely associated with nutrient deficiencies.
  Nurjama yah , Yetti Marlida , Arnim and Yuherman
  Rimbo Panti hot springs located bordering the province of West Sumatra and North Sumatra, precisely in the District of East Pasaman Pasaman regency of West Sumatra Province approximately 200km from the city of Padang. Hot water samples obtained from 5 pools which have a normal temperature of 50-95°C. The aim of this research was to isolate and identify Lactic Acid Bacteria (LAB) thermophilic activity from hot spring with pathogen bacteria (Escherichia coli O157: H7, Salmonella thypmurium and Listeria monocytogenes) followed identified by PCR. The bacteria isolated were growth on medium thermus cair and then deMan Rogosa and Sharpe (MRS) Agar supplement with CaCO3 1% and then performed purification by plate out on deMan Rogosa and Sharpe (MRS) Agar. It was found 23 isolates of LAB showed with clear zone around the culture and 5 isolates (N2, N4, N6, N9 and N12) has been antimicrobial activity against the growth of pathogenic bacteria. The results showed that isolates N6 had the highest antimicrobial activity against all bacteria test, with a range of inhibition zone 18-30 mm, gram positive, spore former coccus, non motility and catalase negative. LAB isolates that have the widest diameter of the clear zone continued to test the minimum inhibitory concentration (MIC). MIC values of isolates N6 supernatant against pathogenic bacteria Escherichia coli O157: H7 by 60 and 80% of the bacterial pathogen Salmonella thypmurium and 50% of the bacterial pathogen Listeria monocytogenes. Based on morphological examination and PCR analysis, the isolate N6 was primarily identified as Pediococcus pentosaceus strain A24 bacteria.
  A. Yuniza and Yuherman
  This research was conducted to determine the best extraction method to produce cinnamon leaf (Cinnamomum burmanii) and noni fruit and leaf (Morinda citrifolia L) mixture extract as source of phytochemical compound to replace the role of antibiotic in broiler production. The mixture extract was named as ‘Cinnamononi extract’. There were four different extraction methods in these experiments, i.e. type A: maceration extraction method with aquadest solvent, type B: maceration extraction method with methanol solvent, type C: modified of reflux extraction and type D: combination of reflux and maceration extract. Two experiments were conducted to evaluate phytochemical compound of four types of cinnamononi extract and to examine antibacterial activity in these extracts. The antibacterial activity of these extracts on Escherichia coli and Salmonella typhimurium were determined using agar ditch diffusion method. The experiment 2 was designed as completely randomized design (CRD) with 5 times replications. There were 9 treatments in this experiment, i.e.: T = antibiotic tetracycline 0.02 g/ml, A1 = cinnamononi extract type A with dilution concentration 1 g/ml, A2 = type A, concentration 0.1 g/ml, B1 = type B, concentration 1 g/ml, B2 = type B, concentration 0.1 g/ml, C1 = type C, concentration 1 g/ml, C2 = type C, concentration 0.1 g/ml, D1 = type D, concentration 1 g/ml, D2 = type D, concentration 0.1 g/ml. Variable in this experiment was inhibition diameter of zone (clear zone) produced after incubation. The result of experiment 1 showed that strong compound containing cinnamononi extract type A, C and D were phenolic (+++), whereas type B was triterpenoid (+++) and negative flavonoid (-). The result of experiment 2 showed that treatments have highly significant effect (p<0.01) to zone of inhibition of E. coli and Salmonella sp. Antibacterial activity of all cinnamononi extract with concentration 0.1 g/ml could replace the role of tetracycline to inhibit Salmonella sp, but to inhibit Escherichia coli higher concentration was needed, i.e., 1 g/ml. In conclusion, type A and C of cinnamononi extract had the best activity to inhibit Escherichia coli bacterial, but only type C of cinnamononi extract which have the best activity to inhibit Salmonella sp.
  Khalil , Reswati , Y.K. Fitri , Indahwati and Yuherman
  Background and Objective: Grazing pasture is presumably able to fulfill fodder requirement for a greater population of breeding cattle, whereas low reproductive rates are most likely caused by mineral deficiency in soil and grazing forages. The present study aimed to evaluate seasonal availability and nutrient composition of pastures in relation to carrying capacity and mineral concentrations of imported breed cattle by considering the mineral profiles of soils, forages and blood plasma samples. Materials and Methods: Samples of forage and soils were collected from 75 sampling points at 15 pasture paddocks during wet and dry seasons. Sample of forages were used for estimation of botanical composition of planted forage species, biomass production and carrying capacity and then analyzed for Dry Matter (DM), crude nutrient, fiber fraction and minerals (Ca, P, Mg, Fe, Mn, Cu, Se and Zn) the same minerals were also analyzed in soil samples. Blood samples were collected from 15 female Simmentals, including heifers and pregnant and non-pregnant cows of each 5 animals. Blood plasma samples were analyzed for Ca, P, Mg, Fe, Mn, Cu, Se and Zn. Results: Pastures were dominated by the Brachiaria decumbens species of approximately 81-84%, while legumes were scarce (0.1-03%). Biomass production carrying capacity and fiber content of forages were significantly higher in the wet season, while DM and crude protein contents were higher in the dry season. Minerals of forages were not significantly affected by the seasons, some micro minerals Mn, Se, Cu and Zn were deficient in soil, forages and cattle. Conclusion: The stocking rate of the grazing pasture could be increased and that dietary supplementation of micro minerals (Mn, Cu, Se and Zn) is needed.
  Sri Melia , Endang Purwati , Yuherman , Jaswandi , Salam N. Aritonang and Mangatas Silaen
  Background and Objective: Listeria monocytogenes is an important pathogenic bacteria in various cases of poisoning in the food industry due to its ability to grow in cold temperatures and to survive in freezing temperatures. Lactic acid bacteria have important probiotic attributes including their antimicrobial effect against this pathogen. Therefore, this study aimed to isolate lactic acid bacteria from buffalo milk and characterize its antimicrobial activity against Listeria monocytogenes. Materials and Methods: Buffalo milk was collected from four districts in West Sumatera, Indonesia and its composition analysed. A total of 88 lactic acid bacteria strains were isolated and grown at De Man Rogosa Sharpe Agar (MRSA). The strains were identified based on morphology (shape, size and colour) and their biochemical characteristics (catalase test and the fermentation type) and then screened for antimicrobial activity against L. monocytogenes. The species were further identified based on 16S rRNA gene sequence analysis. Results: As a result of isolation and identification, 19 strains of lactic acid bacteria were screened against L. monocytogenes, but only three isolates (A 3.2, A 3.3 and TD 7.2) showed high inhibition against L. monocytogenes. They were identified using 16S rRNA gene sequence analysis. Conclusion: The BLAST results of the identification procedure showed that the isolated bacteria from buffalo milk belonged to Lactobacillus fermentum strain L 23 (A 3.3), Lactobacillus fermentum strain 6704 (TD 7.2) and Lactobacillus oris strain J-1 (A 3.2).
  Afriani , Arnim , Yetti Marlida and Yuherman
  Background and Objective: Proteases are important enzymes and have high economic value due to their wide applications in the food industry as a meat tenderizer. Protease use in the food industry necessitates an understanding of the capabilities and influencing factors of these enzymes to accelerate enzymatic reactions. This study aimed to isolate and characterize the proteases of lactic acid bacteria (LAB) from Bekasam. Methodology: The samples were obtained from the third, fifth, seventh, ninth and eleventh day of fermentation to isolate the proteolytic LAB. Characterization of proteases includes the incubation time, casein substrate concentration, optimum temperature and pH, metal ion contents and stability. The LAB with the highest protease activity is identified molecularly and isolated through 16S rDNA sequencing and phylogenetic analysis based on the Neighbor Joining method. Results: The results showed that the best isolate was BAF-715 because it had the highest protease activity (18.84 U mL–1) at 40 h of incubation. The optimum activity of this protease on a casein substrate at 2.5% occurred at an incubation temperature of 40°C at pH 7 and in the presence of Mg2+ and Mn2+ (5 mM) as activators. Based on molecular DNA identification, the BAF-715 isolate is determined to be Pediococcus pentosaceus. Conclusion: A protease produced by Pediococcus pentosaceus showed the highest proteolytic activity, making it the best protease for application as a beef tenderizer.
  Indri Juliyarsi , Puji Hartini , Yuherman , Akmal Djamaan , Arief , Hendri Purwanto , Salam N. Aritonang , James Hellyward and Endang Purwati
  Background and Objective: Tempoyak is a traditional fermented condiment made from durian (Durio zibethinus) pulp. This condiment is made by mixing the durian pulp with salt and fermenting under partially anaerobic conditions at ambient temperature in a closed container. This study aimed to determine the characteristics of Lactic Acid Bacteria (LAB) in tempoyak from Padang Pariaman District, West Sumatra. Methodology: Experimental methods of measuring the chemical composition of tempoyak; isolation and purification of LAB; characterization of selected isolates; isolation of genomic 16S rRNA; microscopic, macroscopic, molecular identification and determination of antimicrobial action by the agar well diffusion method using E. coli, S. aureus and L. monocytogenes as indicator bacteria. Results: Tempoyak is composed of water, protein, fat and has an acidic taste due to its low pH (3.89) affects the water content (70.21%), protein content (5.04%) and fat content (6.11%). Gram staining showed that the isolated bacteria (bacil) are Gram-positive and catalase-negative. Moreover, antimicrobial activity was tested by the agar well diffusion method using E. coli, S. aureus and L. monocytogenes as indicator bacteria. The largest inhibitory zone was observed between the isolated Tempoyak Original (TO) sample and S. aureus (19.3 mm), followed by TO against L. monocytogenes (17.3 mm) and the smallest inhibitory zone was observed between the TO sample and E. coli (12.3 mm). Conclusion: The length of the PCR amplified DNA fragment was 1482 bp. The sequencing results from the isolated tempoyak TO showed that the LAB isolate was Lactobacillus fermentum strain CAU6337.
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