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Articles by Yuhao Wang
Total Records ( 2 ) for Yuhao Wang
  Xun Wang , Haifeng Liu , Ling Zhao , Han Dong , Yuhao Wang , Di Sun , Yuyin Liu , Ruqi Yang and Xuewei Li
  To study the effects of Crude Polysaccharides from Catathelasma ventricosum (CVCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture media contained different concentrations of CVCP (0, 150, 300 and 600 μg mL-1) for 24 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Nile Red staining on days 8 after treatment with culture media supplemented with different concentration CVCP (0, 150, 300 and 600 μg mL-1). As the results, the different concentration CVCP (150, 300 and 600 μg mL-1) promoted proliferation of 3T3-L1 preadipocytes when compared with the control (p<0.05). And it stimulated cell differentiation into adipocyte at low concentration (150 μg mL-1) while it inhibited cell differentiation into adipocyte at higher concentration (300 and 600 μg mL-1). But the difference was not significant (p>0.05). Besides, lipid content in 3T3-L1 cells treated with 150 μg mL-1 was significantly higher than treated with 600 μg mL-1 (p<0.05). It can be concluded from the results that the CVCP promoted proliferation of the 3T3-L1 cells 24 h after treatment. Meanwhile, it was involved to some extent in regulation of adipogenesis and lipogenesis.
  Xun Wang , Hengmin Cui , Haifeng Liu , Ling Zhao , Xi Peng , Hengyong Xu , Yan Wang , Yuhao Wang , Ling Niang and Qian Rong
  To study the effect of the crude polysaccharides from Poria cocos (PCCP) on mRNA expression of adipogenic factors during 3T3-L1 preadipocyte differentiation. 3T3-L1 preadipocytes were induced to differentiate with MDI (3-isobutyl-1-Methylxanthine, Dexamethasone and Insulin) induction media contained PCCP (50 μg mL-1) and cultured for 8 days. The extent of the differentiation was assessed by Nile Red staining and GPDH activity. In addition, researchers used real-time quantitative PCR to analyzed the expression of genes related to adipocyte differentiation. The results showed that treatment of differentiating 3T3-L1 preadipocytes with PCCP (50 μg mL-1) resulted in an increase in the expression of the adipocyte genes such as Fatty Acid System Synthase (FAS), adipocyte fatty acid binding Protein (aP2), insulin-responsive Glucose Transporter (GLUT4) and transcriptional factor genes such as CCAAT/Enhancer Binding Protein α (C/EBPα), peoxisome Proliferator-Activated Receptor gamma (PPARγ) and Sterol Regulatory Element-Binding Protein 1c (SREBP-1c) to some degree. Overall, the data suggested that there was the tendency of increasing the expression of genes related to adipocyte differentiation in PCCP treated cells.
 
 
 
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