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Articles by Yugang Gao
Total Records ( 3 ) for Yugang Gao
  Guangsheng Xi , Jianing Wang , Ping Li , Yugang Gao , Shengxue Zhou and Yunjia Wang
  Background and Objective: The low capacity of body in the absorption of ginsenosides presents an important question as how to effectively improve absorption, which remains a subject of broad and current interest among researchers. This study aimed to investigate the effects of compound amino acids and ginsenosides on the visceral organ index and blood lipid profile and to assess the absorption rate of ginsenosides and amino acids. Materials and Methods: Male iCR mice were randomly assigned to 7 groups (12 mice/group): Group i (saline control), Group ii (ginseng), Group iii (composite ginsenosides), Group iV (compound amino acids), Group V (amino acids and ginsenosides, high dose), Group Vi (amino acids and ginsenosides, middle dose) and Group Vii (amino acids and ginsenosides, low dose) who were treated with the corresponding drugs by gavage for 4 weeks. Weight, visceral index, blood lipid profiles and absorption rates of the mice were then analysed and compared using one-way ANOVA. Results: The middle dose of compound amino acids and ginsenosides increased the body weight of the mice without influencing the liver, lung, kidney, testis, spleen or thymus indexes (p>0.05). Compound amino acids and ginsenosides reduced blood lipid levels, including CHO, TG, HDL-C and LDL-C. HPLC and amino acid analyses revealed that compound amino acids could significantly improve p<0.05 the absorption rate of ginsenosides in mice (p<0.05), especially in the middle-dose group. Conclusion: Supplemental compound amino acids may be helpful for ginsenoside uptake by visceral organs. in addition, the beneficial effects of ginsenosides include body weight increase and blood lipid reduction.
  Guofeng Zhang , Shengxue Zhou , Hai Zhao , Guangsheng Xi , Yugang Gao and Yunjia Wang
  Background and Objective: Ginsenosides are considered as key components to mediate the pharmacological activity of the ginseng. It is great significance for obtaining ginseng extract containing high concentration of ginsenosides. This study aimed to investigate the effects of rolC gene on ginsenosides content in ginseng hairy roots. Materials and Methods: Genomic DNA was extracted from ginseng hairy roots of Panax ginseng C.A. Meyer (P. ginseng) and rolC gene was cloned for pMD18-T/rolC recombinant plasmid construction. Then Agrobacterium rhizogenes A4 harboring rolC gene was used to infect the ginseng hairy roots obtained after inoculating root of P. ginseng. The mRNA and protein of rolC gene were then detected and the different types of ginsenosides were evaluated. Results: A 543 bp length DNA was cloned from ginseng hairy roots and pMD18-T/rolC recombinant plasmid was successfully constructed for rolC gene introduction. Increased growth ratio was found in rolC gene expressed ginseng hairy roots than 3 years old ginseng roots. Moreover, the highest total ginsenosides level (16.13 mg g–1) was found in ginseng hairy roots at 4 weeks, which was increased to 355% than 3 years old ginseng (4.54 mg g–1). Increased levels of both ginsenosides Rb (Rb1, Rb2, Rc, Rd) and Rg (Re, Rf and Rg1) subgroup were found from 2-8 weeks in ginseng hairy roots and 4 week was the optimal time point for the highest ginsenosides content. Conclusion: Introduction of rolC gene could promote ginsenosides content in ginseng hairy roots, which would be beneficial for obtaining ginseng extract containing high concentration of ginsenosides.
  Yugang Gao , Xueliang Zhao , Pu Zang , He Yang , Tong Liu , Qun Liu and Lianxue Zhang
  Bovine Viral Diarrhea Virus (BVDV), a single-stranded RNA virus can cause fatal diarrhoea syndrome, respiratory problems and reproductive disorder in herd. However, there still no effective vaccine or medicines for controlling the spreading of BVDV. In this study, researchers are interested in obtaining proteins with anti-BVDV activity in ginseng. The structure protein E0 of BVDV was used as bait protein to screen the ginseng cDNA library by the yeast two hybrid assay system. The result showed that eukaryotic translation initiation factor 5A (eIF5A) of ginseng has direct interaction with E0. The interaction was verified by GST-pull down analysis. This interaction provides a target for researching of antiviral drugs for controlling the spreading of BVDV.
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