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Articles by Yuda Heru Fibrianto
Total Records ( 4 ) for Yuda Heru Fibrianto
  Teguh Budipitojo , Yuda Heru Fibrianto and Bambang Sumiarto
  Background and Objective: The gastrin-releasing peptide (GRP) has been proposed as an autocrine and/or paracrine growth factor in the female reproductive tract based on the presence of GRP immunoreactivity in the reproductive tract of human, sheep and cow but unproven at the cellular level. Since, GRP mediates their important functions by binding to cell surface gastrin-releasing peptide receptor (GRPR), the present study was aimed to clear out the mitogenic activity of GRP in the placenta of the cow based on the immunolocalization of GRPR and Proliferating Cell Nuclear Antigen (PCNA) at the cellular level. Materials and Methods: Three placental tissues of 7 months pregnant cows were used for light microscopic immunohistochemical staining using the polymerized reporter enzyme staining system. Results: Immunohistochemical results showed that GRPR immunoreactivity was found in the cell membrane of mononucleated trophoblast cells, while PCNA immunoreactivity was detected in the nuclear part of mononucleated trophoblast cells. No GRPR and PCNA immunoreactivity was visualized in both mononucleated and multinucleated trophoblast giant cells. Gastrin-releasing peptide receptor and PCNA immunoreactive cells were also not detetcted in the maternal epithelium of 7 months placental tissues of the cows. Conclusion: The present results suggested that in the placental tissues of 7 months pregnant cows, GRP plays mitotic roles to promote proliferation and differentiation of mononucleated trophoblast cells via autocrine and paracrine loops as indicated by the presence of GRPR and PCNA immunoreactivities in the mononucleated trophoblast cells of the present study and the existance of GRP immunoreactivities in the trophoblast and both mononucleated trophoblast cells and multinucleated trophoblast giant cells of the former studies.
  Teguh Budipitojo , Yuda Heru Fibrianto and Guntari Titik Mulyani
  Background and Objective: The present study was conducted to determine the histological structure of mucosa stomach in adult sunda porcupine (Hystrix javanica) which is endemic to Indonesia. Sunda porcupine is an important vertebrate pest in Indonesia’s agriculture, but there is little knowledge of stomach structure of this pest. Materials and Methods: During the present study, five Hystrix javanica were used to examine the structure of mucosa stomach, microscopically by using hematoxylin-eosin and alcian blue-periodic acid Schiff’s staining methods. Results: Histologically, the wall of the tunica mucosa stomach consists of three laminas, lamina epithelialis mucosa, lamina propria mucosa and lamina muscularis mucosa. The stomach covered by thick phytobenzoars-like structure which fused with simple short columnar epithelium that extends through gastric pits, where the gastric glands are opened. Three regions can be recognized in the mucosa of the stomach according to the types of glands that appear as tubular short glands with bent portion at the basal in cardiac region, tubular branched and coiled glands in fundic glands region while, the pyloric glands region appear as simple branched tubular long straight over most of their length parallel to each other. The cardiac glands noted mainly serous secreting cells while the fundic glands region consist of different types of cells but the abundance main cells is the parietal cells. Pyloric glands region consist of two main cells are the parietal and chief cells, but the number of parietal cells dramatically decrease compare with fundic glands region and gradually dissapear in the end of the region. Conclusion: The tunica mucosa stomach of Hystrix javanica consists of three lamina (lamina epithelialis mucosa, lamina propria mucosa and lamina muscularis mucosa) and divided into three gland regions (richly neutral mucopolysacarides producing cells of cardiac, acidic mucopolysacarides producing cells of fundic, mixed acid and neutral mucopolysacarides producing cells of pyloric glands regions) covered by neutral mucopolysacarides of thick phytobenzoars-like structures.
  Irma Padeta , Widagdo Sri Nugroho , Dwi Liliek Kusindarta , Yuda Heru Fibrianto and Teguh Budipitojo
  Background: Mesenchymal stem cell-conditioned medium (MSC-CM) is the substance extracted from mesenchymal stem cell culture, contains a lot of potential cytokines and growth factors as regenerative agent. Objective: The aim of this study was to investigate the role of MSC-CM on the burn wound regeneration pattern of white rat (Rattus norvegicus ). Methodology: Rat was anesthetized using combination of 10% ketamine and 2% xylazine at the dose rate of 75 and 5 mg kg–1 b.wt., respectively. The burn wound condition was created on the dorsal area of each rat. The burn wound area of control group was treated with Bioplacenton®, while MSC-CM group was topically treated with MSC-CM cream twice a day. The diameter of burn wound was measured every 5 days from wounded. Skin wound tissues were collected at 4 h, followed by 2, 5, 10, 15, 20, 25 and 30 days after wounded and then were processed by paraffin method. Tissue samples were visualized by using hematoxylin-eosin and Masson’s trichrome stain. The polymer-based immunohistochemistry method was employed to detect the presence of basic fibroblast growth factor (bFGF) as important growth factor in wound healing process. Result: The results showed that MSC-CM promote the recovery of skin burn wound in white rat, as indicated by: (1) Acceleration of wound closure, (2) Greater numbers of fibroblasts, (3) High density of collagen fibers and (4) Greater numbers of blood vessels in MSC-CM group compare with control group. Conclusion: Since the number of bFGF immunoreactive cells increased significantly during recovery proccess in MSC-CM group compare with control group, it was suggested that bFGF plays important role on the tissues regeneration of skin burn treated by MSC-CM.
  Teguh Budipitojo , Yuda Heru Fibrianto and Guntari Titik Mulyani
  Background and Objective: The regional distribution and relative frequency of gastrointestinal endocrine cells has been found to vary with the animal species and feeding habits. This study aimed to analyze the existence and distribution of immunoreactive endocrine cells to chromogranin, ghrelin, somatostatin, serotonin and gastrin in the gastric mucosa of sunda porcupine (Hystrix javanica ) using immunohistochemistry. Materials and Methods: Five adult sunda porcupines were used without sexual distinction. The presences of endocrine cells were visualized by ABC immunohistochemical methods using five types of polyclonal antisera, specific for chromogranin, ghrelin, somatostatin, serotonin and gastrin. Results: In the stomach of sunda porcupine, endocrine cells immunoreactive for all of these markers were observed. The chromogranin-immunoreactive cells were found with greatest frequency, in all stomach regions. In all regions of the stomach, endocrine cells secreting ghrelin were also detected in weaker frequency compare with chromogranin. The somatostatin immunoreactive cells were detected rare in the gastric and fundic regions, serotonin cells were found rare in fundic but few in pyloric regions, while gastrin cells were detected abundance in pyloric region of stomach of the sunda porcupines. Gastrin positive cell was also detected in the duodenal part of sunda porcupine. The chromogranin-positive cells were the most numerous, being more prevalent in the stomach glands. The other endocrine cells were identified in smaller numbers, some of them located in all regions or in specific regions. The finding of these cell types in the mucosal stomach confirms their preferential location in the final portions of the principal regions of the stomach and suggests control by feedback of its functions. Conclusion: In conclusion, the present study show for the first time the immunolocalization of chromogranin, ghrelin and gastrin immunoreactive cells in the stomach of sunda porcupine. However, in contrast with previous finding of crested porcupine and other rodents, the present result found serotonin immunoreactive cells in the fundic and pyloric gland regions and somatostatin immunoreactive cells in the gastric and fundic gland regions of sunda porcupine stomach. The differences may was caused by feed types, feeding habits and geographical locations of the species.
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