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Articles by Yu-Ming Wei
Total Records ( 5 ) for Yu-Ming Wei
  Li-Juan Xiong , Wei Li , Yu-Ming Wei and You-Liang Zheng
  In order to exploit new genetic resources and provide fundamental materials for the breeding improvement of bread wheat quality, genetic variation of high-molecular-weight glutenin subunits and gliadin in 29 macha wheat accessions were observed by acidic polyacrylamide-gel electrophoresis and sodium dodecyl sulphate polyacrylamide-gel electrophoresis. Nine HMW-glutenin subunits alleles and 9 combinations were identified. Subunit null (82.8%), 7+8 (53.3%) and 2+12 (82.8%) scored the highest frequency at Glu-A1, Glu-B1 and Glu-D1 loci, respectively. In addition, subunits 7+9 (23.3%) was also found at higher frequency. A total of 49 gliadin bands and 28 patterns were detected and the polymorph among all accessions was identified in most of bands (97.96%). Furthermore, all materials could be clustered into three major groups based on genetic similarity coefficient. The results indicated that the variations of gliadin among macha accessions were not associated with their geographic origins.
  Zhuo Huang , Hai Long , Yu-Ming Wei , Ze-Hong Yan and You-Liang Zheng
  In this study, a pair of degenerate PCR primer was designed base on the known LMW-GS genes, with which 4 distinct sequences were obtained from a weak gluten variety of wheat, Chuannong 16, designated as LMWCN16-1, LMWCN16-2, LMWCN16-3 and LMWCN16-4, respectively. The total lengths of the 4 genes were 902, 915, 909 and 927 bp, respectively. All these genes were predicted to be located at Glu-D3 locus and amount of DNA variations were found, including 97 nucleotide substitutions and several insertions and deletions (indels). Sequence analysis indicated that the pairwise identities between the 4 genes were from 81.9 to 95.6%. LMWCN16-2 and LMWCN16-3 were more close related, while LMWCN16-4 was distinguished from LMWCN16-2 and LMWCN16-3 by a longer repetitive domain and the different position of the seventh cysteines involved in inter-molecular disulphide bond. These differences might result in different effects on the formation of the glutenin polymer and consequently the wheat quality. LMWCN16-1 is a putative pseudogene due to the five inframe stop codons and the frame shifting mutation in its C-terminal domain. The function loss of LMWCN16-1 might weaken the proportion of Glu-D3 in the total LMW-GS and bring negative influence on wheat quality.
  Ze-Hong Yan , Shou-Fen Dai , Deng-Cai Liu , Yu-Ming Wei and You-Liang Zheng
  The HMW glutenin subunit (HMW-GS) allelic variations of two hundred and twenty nine Tibetan wheat landraces were analyzed by using SDS-PAGE analysis. Seventeen different allelic variations were detected in the evaluated accessions, which were three alleles at the Glu-A1 locus, nine alleles at the Glu-B1 locus and five alleles at the Glu-D1 locus, respectively. Two novel HMW-GS, designated as 5** at the Glu-D1 in As1243 and 6** at the Glu-B1 locus in As1510, were screened out. Based on the present results and previous results, it was suggested that the HMW-GS combinations null, 7+8, 2+12, is the predominate types in all the Chinese wheat landraces. It was noteworthy that 5+10, the generally accepted HMW-GS pairs endowing wheat with good bread making quality at Glu-D1, could be detected in Tibetan wheat landraces, which have been found very rare in other Chinese wheat landraces. Additionally, the rare subunit combinations 2+10 and 2.1+10.1 were respectively found in Tibetan wheat landrace.
  Ya-Xi Liu , Wei Li , Yu-Ming Wei , Guo-Yue Chen and You-Liang Zheng
  This study characterizes 15 waxy genes from 15 accessions of the einkorn wheats Triticum urartu, T. boeoticum and T. monococcum. The mature protein coding sequences of waxy genes were analyzed. Nucleotide sequence variations in these regions resulted from base substitution and/or indel mutations. This work identified 8 distinct haplotypes from the diploid wheat waxy gene sequences. A main haplotype was found in 7 gene samples from the Au genome and Am genome. The waxy gene sequences from the Au and Am genomes could be obviously clustered into two clades, but the sequences from the Am genome of T. boeoticum and T. monococcum could not be clearly distinguished. The phylogenetic analysis revealed that the waxy gene sequences from the Am genome had accumulated fewer variations and evolved at a slower rate than the sequences from the Au genome. These results would contribute to the understanding of functional aspects and efficient utilization of waxy genes.
  Zhuo Huang , Hai Long , Yu-Ming Wei and Ze-Hong Yan
  In this study, PCR primers were designed base on the known genes in wheat and its relatives to isolate α-gliadin genes from Ae. sharonensis, one species of the Sitopsis section of the genus Aegilops, which was traditionally considered as the B-genome donor of tetraploid and hexaploid wheat. Three novel α-gliadin genes were obtained. Analyses of the nucleotide and deduced amino acids of the obtained genes indicated that they shared the high sequence identities and similar primary structures to the known α-gliadin genes. Further more, the extensive variations were found. A phylogenic analysis based on the multigene alignment of the deduced amino acid sequences showed that the α-gliadin genes derived from Ae. sharonensis and Ae. speltoides were significantly distinguished from those of diploid A and D genome progenitors of wheat, but clustered closed to some genes from tetra- and hexaploid wheats. Moreover, genes derived from Ae. sharonensis were more closed to wheat than from Ae. speltoides. This result suggested that the origin and formation of wheat B genome might be polyphyletic.
 
 
 
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