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Articles by Yovita Astuti Djohan
Total Records ( 1 ) for Yovita Astuti Djohan
  Gede Suantika , Alissa Diany Putri , Yovita Astuti Djohan , Fahma Fiqhiyyah Nur Azizah , Dea Indriani Astuti and Pingkan Aditiawati
  Background and Objective: The impact of environmental factors on the B vitamins (B1, B2, B6 and B12) content of marine diatom Skeletonema costatum has not yet been evaluated so far. Here, we aim to optimize the B vitamins production (vitamin B1, B2, B6 and B12) through culture of S. costatum on different salinity levels as well as light intensity exposures. Materials and Methods: The marine diatom Skeletonema costatum was cultured in different salinity levels (24, 29 and 34 g L–1) to optimize B vitamins production, prior to exposure to different light intensity levels (20, 34 and 47 μmol m–2 sec–1) for 3 days in f/2 medium. Results: Twenty four grams per liter salinity exposure produced the highest vitamin B1, B6 and B12 content of 90.08±2.6, 410.03±12.97 and 61.22±27.67 μg g–1, respectively. When cultivated either below or above light intensity of 34 μmol m–2 sec–1, vitamin B1, B6 and B12 content decreased. The highest total B vitamins obtained at 34 μmol m–2 sec–1 was 563.46 mg L–1 (vitamin B1: 90.08±1.48 μg g–1, vitamin B2: 2.87±0.79 μg g–1, vitamin B6: 410.03±12.97 μg g–1 and vitamin B12: 61.22±27.67 μg g–1). Conclusion: Optimum growth, biomass and total B vitamins produced was achieved by culturing at salinity of 24 g L–1 and light intensity 34 μmol m–2 sec–1. So far, optimum growth, biomass and total B vitamins produced was achieved by culturing at salinity of 24 g L–1 and light intensity 34 μmol m–2 sec–1. Note that when aiming at high vitamin productivities, it is better to culture S. costatum in a two step process: A nutrient sufficient phase in optimum environmental growth conditions to produce enough cells (e.g., salinity, illumination, pH, temperature, supply of CO2 and nutrients etc.), followed by suitable stress to stimulate B vitamins synthesis in a controlled manner.
 
 
 
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