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Articles by Youxun Liu
Total Records ( 2 ) for Youxun Liu
  Youxun Liu , Mingyang Yan and Juan Huang
  In order to explore separation and purification methods of Coriolopsis trogii laccase production by solid fermentation, we used a three-phase separation technique and wherein the main factors (ammonium sulfate concentration, t butanol concentration, temperature and pH) were optimized. The results showed that when the three-phase partitioning optimum conditions were as follows: Ammonium sulfate concentration was 40%, tertbutanol water phase volume ratio of 1:1, the temperature is 20°C, pH 5, laccase purification factor and recovery can be up to 20 times and 75%, respectively, SDS-PAGE electrophoresis showed that the purified protein is substantially a single band and a molecular weight of about 38 kD, smaller than the known fungal laccase molecular weight; the results prove that the three-phase separation from the crude enzyme solution Coriolopsis trogii solid obtained after fermentation relatively pure laccase, eliminating the need for complex chromatography step, the method is simple and low cost, for a lot of separation and purification of laccase provide practical foundation.
  Yun Fu , Yu Zhang , Sufeng Zhou , Youxun Liu , Jiangang Wang , Yali Wang , Chengbiao Lu and Changzheng Li
  Ciprofloxacin is one of fluoroquuinones widely used as antibiotics in clinical treatments. It has been shown that the carboxyl group on position 3 of ciprofloxacin is essential for antimicrobial activity, however, the amide form on this position and its corresponding biological effects have not been studied. To determine the structure-activity relationship, the ciprofloxacin hydrazide derivative was synthesized and its antimicrobial and antitumor mechanism was also evaluated preliminarily. Our results demonstrated that the substitution of -OH of carboxyl on position 3 of ciprofloxacin with a -NH-NH2 group could slightly alter the antimicrobial spectra, but not significantly. The studies of the hydrazide derivative on cell cycle arrest, mitochondrial membrane permeability, topoisomerase inhibition, pro-apoptotic gene regulation and molecular docking revealed that the minor structural modification on position 3 of ciprofloxacin did not result in changes in molecular targets compared to ciprofloxacin.
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