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Articles by Younes Ghasemi
Total Records ( 6 ) for Younes Ghasemi
  Younes Ghasemi , Alireza Ebrahiminezhad , Sara Rasoul-Amini , Gholamreza Zarrini , Mohammad Bagher Ghoshoon , Mohammad Javad Raee , Mohammad Hossein Morowvat , Farshid Kafilzadeh and Aboozar Kazemi
  Purified L-asparaginase II from Escherichia coli has been supplied and employed in the acute leukemia and other malignant neoplasms chemotherapy. L-asparaginase II gene (ansB) in E. coli is under regulation and certain conditions is needed for expression of this gene. In this investigation ,the various concentrations of modified M9 medium ingredients and various carbon source were tested to optimize the medium for expression and identification of L-asparaginase in E. coli. Finally a semi-quantitative plate assay for L-asparaginase producing Escherichia coli is reported.
  Younes Ghasemi , Sara Rasoul-Amini , Mohammad Hossein Morowvat , Seyed Bagher Mosavi Azam , Shadman Shokravi , Abdolali Mohagheghzadeh , Mohammad Bagher Ghoshoon and Mohammad Javad Raee
  A unicellular microalga, Oocystis pusilla, was isolated from paddy-field and applied in the biotransformation experiment of hydrocortisone (1). This strain has not been previously tested for steroid bioconversion. Fermentation was carried out in BG-11 medium supplemented with 0.05% substrate at 25 °C for 14 days incubation. The products obtained were chromatographically purified followed by their characterization using spectroscopic methods. 11β, 17α, 20β, 21-tetrahydroxypregn-4-en-3-one (2), 11β, 17β-dihydroxyandrost-4-en-3-one (3) and 11β-hydroxyandrost-4-en-3, 17-dione (4) were the main byproducts in the hydrocortisone bioconversion. Bioreaction characteristics observed were 20-ketone reduction for accumulation of compound 2 and side chain degradation of the substrate to prepare compounds 3 and 4. Time course study showed the accumulation of the product 2 from the second day of the fermentation and 3 as well as 4 from the third day. All the metabolites reached their maximum concentration in seven days. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 0.5 mg mL-1 in one batch. Growth was not influenced by the addition of steroid substrate. Biotransformation was completely inhibited in a concentration above 2.0 mg mL-1.
  Younes Ghasemi , Amir Khalaj , Abdolali Mohagheghzadeh , Ahmad Reza Khosravi and Mohammad Hossein Morowvat
  HOFARIGHUN, RAEE flower, thousand eyes wort are popular names for Hypericum sp in Persian language mostly called H. perforatum. It has been used as antispasmodic, diuretic, antimigraine, antiepileptic and cholagouge. Tisane of these plants in red wine was used as snake bite and burning remedy. The volatile constituents, obtained from air-dried aerial parts of fruiting Hypericum elongatum were analyzed by GC/MS method. Thirty four components of about 96.50% of total oil were identified. Pinene <α> (80.43%), Terpinene <γ> (4.23%) and Pinene <β>(2.59%) were the principal components (87.16%). The essential oil and hydroalcoholic extract were evaluated for antibacterial, antifungal and anti-yeast activities by using disc diffusion method. Screening of the antimicrobials was investigated on Gram positive bacteria (Staphylococcus aureus PTCC 1112, Staphylococcus epidermidis PTCC 1114, Bacillus subtilis PTCC 1023, Enterococcus faecalis ATCC 8043), Gram negative bacteria (Escherichia coli PTCC 1338, Pseudomonas aeruginosa PTCC 1047, Salmonella typhi PTCC 1609), yeasts (Candida albicans ATCC 14053, Candida kefyr ATCC 3826) and fungi (Aspergillus niger PLM 1140, Aspergillus fumigatus PLM 712). The MIC of essential oil also was identified. Antimicrobial activity of essential oil against all of the microorganisms was observed, except Aspergillus niger and Aspergillus fumigatus. In spite of antimicrobial activity of hydroalcoholic extract against bacteria, there was no antimicrobial activity against fungi and yeasts. A survey of the literature revealed no reports dealing with chemical composition of essential oil and antimicrobial activity of Hypericum elongatum.
  Younes Ghasemi , Ameneh Moradian , Abdolali Mohagheghzadeh , Shadman Shokravi and Mohammad Hossein Morowvat
  Antifungal and antibacterial activity of the microalgae from paddy fields in the south of Iran were studied. Soil samples were collected from paddy fields of Fars province and were cultured in BG11 medium. Supernatants, methanolic and hexane extracts from biomass of 60 strains of microalgae were isolated and screened against six strains of bacteria and four strains of fungi. The culture supernatants of 21 strains of microalgae and methanolic extracts of 8 strains exhibited significant antibacterial effect and 17 strains showed antifungal effect. No antimicrobial activity was detected in the hexane extracts and no methanolic extracts inhibited the growth of fungi. In present screening, Chroococcus dispersus, Chlamydomonas reinhardtii and Chlorella vulgaris appeared to be the most promising strains and it was shown that they excreted a broad spectrum of antimicrobial substances in the culture medium. Among all of the species studied in this investigation for antibacterial and antifungal activity, Chroococcus dispersus PTCC 1677 indicated widespread spectrum of antimicrobial activities. Bioautography and Bioassay-guided fraction of culture medium of the Chroococcus dispersus exhibited a polar substance in the culture medium as well.
  Zahra Moradpour , Maryam Torshabi , Mohammad Ali Faramarzi , Mojtaba Tabatabaei Yazdi , Younes Ghasemi , Hoda Jahandar , Nadia Zolfaghary and Gholamreza Zarrini
  The ability of Nostoc ellipsosporum PTCC 1659, a cyanobacterium strain, for biotransformation of androst-4-en-3,17-dione (AD) was studied. Fermentation was performed in BG-11 medium supplemented with 0.05% AD at 25°C for seven days incubation. The single metabolite obtained was purified using chromatographically methods and characterized as testosterone on the basis of its spectroscopic features. Bioreaction characteristic observed was 17-carbonyl reduction. Time course study showed the accumulation of the product from the third day of the fermentation and reached to the maximum in the seventh day. Production of testosterone was not affected by aeration. Continuously illumination or 16 h light/8 h dark has no effect on the transformation yield. Optimum concentration of the substrate, which gave maximum bioconversion efficiency, was 0.5 mg mL-1 in one batch. Biotransformation was completely inhibited in a concentration above 2.0 mg mL-1.
  Zahra Moradpour , Maryam Torshabi , Mohammad Ali Faramarzi , Mojtaba Tabatabaei Yazdi , Younes Ghasemi , Hoda Jahandar , Nadia Zolfaghary and Gholamreza Zarrini
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