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Articles by Yong LI
Total Records ( 9 ) for Yong LI
  Feng Zhang , Jiali Zhang , Meihong Xu , Xiaoqian Dai , Yujie Li , Ye Ding , Zhaofeng Zhang and Yong Li
  The present study aimed to investigate the effects of Corn Oligopeptides (COP) on acute alcoholism in rat models. Female SD rats were randomly assigned to 4 groups per test including control group and 3 COP intervention groups (2.25, 4.5 and 9 g/kg/BW). About 5 g/kg/BW ethanol (8 g/kg/BW for loss of righting reflex test) was given intragastricly 10 min before COP treatment. Ethanol levels in blood, behavioral studies and ADH activity in liver were assayed. Researchers found that COP treatment could promote the alcohol metabolism and correct the movement disorder caused by alcohol consumption to some extent. However, the ADH levels had no changes among different groups. These findings suggest that COP have a protective effect on acute alcoholism in rats.
  Ramona Rozen , Narayanan Sathish , Yong Li and Yan Yuan
  Herpesvirus virions are highly organized structures built through specific protein-protein interactions. Thus, revelation of the protein interactions among virion proteins will shed light on the processes and the mechanisms of virion formation. Recently, we identified 24 virion proteins of Kaposi`s sarcoma-associated herpesvirus (KSHV), using a proteomic approach (F. X. Zhu et al., J. Virol. 79:800-811, 2005). In the current study, a comprehensive analysis of protein-protein interaction between KSHV virion proteins was carried out using yeast two-hybrid (Y2H) and coimmunoprecipitation (co-IP) approaches. Every pairwise combination between KSHV tegument and capsid proteins, between tegument and envelope proteins, and among tegument proteins was tested for possible binary interaction. Thirty-seven protein-protein interactions were identified by both Y2H and co-IP analyses. The results revealed interactions between tegument and capsid proteins such as that of open reading frame 64 (ORF64) with ORF25 (major capsid protein [MCP]), ORF62 (triplex-1 [TRI-1]), and ORF26 (TRI-2). Many interactions were detected among the tegument proteins. ORF64 was found to interact with several tegument proteins including ORF11, ORF21, ORF33, ORF45, ORF63, ORF75, and ORF64 itself, suggesting that ORF64 may serve as a hub protein and play a role in recruiting tegument proteins during tegumentation and virion assembly. Our investigation also revealed redundant interactions between tegument proteins and envelope glycoproteins. These interactions are believed to contribute to final envelopment in virion assembly. Overall, this study allows us to establish a virion-wide protein interaction map, which provides insight into the architecture of the KSHV virion and sets up a foundation for exploring the functions of these proteins in viral particle assembly.
  Chieh-Fang Cheng , Jianhua Fan , Mark Fedesco , Shengxi Guan , Yong Li , Balaji Bandyopadhyay , Alexandra M. Bright , Dalia Yerushalmi , Mengmeng Liang , Mei Chen , Yuan-Ping Han , David T. Woodley and Wei Li
  Jump-starting and subsequently maintaining epidermal and dermal cell migration are essential processes for skin wound healing. These events are often disrupted in nonhealing wounds, causing patient morbidity and even fatality. Currently available treatments are unsatisfactory. To identify novel wound-healing targets, we investigated secreted molecules from transforming growth factor α (TGFα)-stimulated human keratinoytes, which contained strong motogenic, but not mitogenic, activity. Protein purification allowed us to identify the heat shock protein 90α (hsp90α) as the factor fully responsible for the motogenic activity in keratinocyte secretion. TGFα causes rapid membrane translocation and subsequent secretion of hsp90α via the unconventional exosome pathway in the cells. Secreted hsp90α promotes both epidermal and dermal cell migration through the surface receptor LRP-1 (LDL receptor-related protein 1)/CD91. The promotility activity resides in the middle domain plus the charged sequence of hsp90α but is independent of the ATPase activity. Neutralizing the extracellular function of hsp90α blocks TGFα-induced keratinicyte migration. Most intriguingly, unlike the effects of canonical growth factors, the hsp90α signaling overrides the inhibition of TGFβ, an abundant inhibitor of dermal cell migration in skin wounds. This finding provides a long-sought answer to the question of how dermal cells migrate into the wound environment to build new connective tissues and blood vessels. Thus, secreted hsp90α is potentially a new agent for wound healing.
  Yong Li , Tomoki Kimura , Ryan W. Huyck , John H. Laity and Glen K. Andrews
  Herein, the mechanisms of transactivation of gene expression by mouse metal response element-binding transcription factor 1 (MTF-1) were investigated. Evidence obtained from coimmunoprecipitation assays revealed that exposure of the cells to zinc resulted in the rapid formation of a multiprotein complex containing MTF-1, the histone acetyltransferase p300/CBP, and the transcription factor Sp1. Down-regulation of endogenous p300 expression by small interfering RNA transfection significantly decreased zinc-dependent metallothionein I (MT-I) gene transcription without altering induction of zinc transporter 1 (ZnT1). MTF-1 independently facilitated the recruitment of Sp1 and p300 to the protein complex in response to zinc. Mutagenesis demonstrated that the acidic domain, one of three transactivation domains of MTF-1, is required for recruitment of p300 but not Sp1 as well as for zinc-dependent activation of MT-I gene transcription. Furthermore, mutation of leucine residues (L>A) within a nuclear exclusion signal in the MTF-1 acidic domain impaired recruitment of p300 and zinc-dependent activation of the MT-I gene. Nuclear magnetic resonance characterization of an isolated protein fragment corresponding to the MTF-1 acidic region demonstrated that this region is largely unstructured in the presence and absence of excess stoichiometric amounts of zinc. This suggests that the mechanism by which MTF-1 recruits p300 to this complex involves extrinsic-zinc-dependent steps. These studies reveal a novel zinc-responsive mechanism requiring an acidic region of MTF-1 that functions as a nuclear exclusion signal as well as participating in formation of a coactivator complex essential for transactivation of MT-I gene expression.
  Deqiang Zhou , Gui Yun Tian and Yong Li
  There is a need for a pulsed eddy current (PEC) to detect very small defects and deep buried subsurface flaws reliably in metallic structures such as aircraft wings. This paper reports an investigation into the sensitivity for a pancake PEC probe to variations in the material conductivity of specimens. Three experimental coil parameters are modelled: (a) coil inner radius, (b) coil width and (c) coil height. Based on the predicted signals, the sensitivity of the PEC probe as a function of coil parameters has been formulated. Through matrix numerical simulation experiments using orthogonal array, the weighting of each parameter to the sensitivity has been derived. Subsequently, a theoretical model for probe optimisation is established in order to realise the maximum sensitivity, based on which a probe is designed with improved sensitivity.
  Aihua Sun , Lin Huang and Yong Li
  TiO2 and polystyrene sulfonic sodium (NaPSS) composite film humidity sensors were prepared through dip-coating method. The polystyrene sulfonic sodium (NaPSS) was dip-coated onto the surface of TiO2 porous film on alumina substrate to fabricate the humidity sensor. The electrical properties of the sensor were measured. The TiO2/NaPSS composite film exhibited better sensing properties than the pure polymer NaPSS humidity sensor, such as smaller hysteresis and quicker response-recovery time.
  Yong Li , Cynthia Suggs , J. Timothy Wright , Zhi- an Yuan , Melissa Aragon , Hanson Fong , Darrin Simmons , Bill Daly , Ellis E. Golub , Gerald Harrison , Ashok B. Kulkarni and Carolyn W. Gibson
  The amelogenins are the most abundant secreted proteins in developing dental enamel. Enamel from amelogenin (Amelx) null mice is hypoplastic and disorganized, similar to that observed in X-linked forms of the human enamel defect amelogenesis imperfecta resulting from amelogenin gene mutations. Both transgenic strains that express the most abundant amelogenin (TgM180) have relatively normal enamel, but strains of mice that express a mutated amelogenin (TgP70T), which leads to amelogenesis imperfecta in humans, have heterogeneous enamel structures. When Amelx null (KO) mice were mated with transgenic mice that produce M180 (TgM180), the resultant TgM180KO offspring showed evidence of rescue in enamel thickness, mineral density, and volume in molar teeth. Rescue was not observed in the molars from the TgP70TKO mice. It was concluded that a single amelogenin protein was able to significantly rescue the KO phenotype and that one amino acid change abrogated this function during development.
  Yong Li , Amanda Kovach , Kelly Suino-Powell , Dariusz Martynowski and H. Eric Xu
  The functional interaction between the peroxisome proliferator-activated receptor γ (PPARγ) and its coactivator PGC-1α is crucial for the normal physiology of PPARγ and its pharmacological response to antidiabetic treatment with rosiglitazone. Here we report the crystal structure of the PPARγ ligand-binding domain bound to rosiglitazone and to a large PGC-1α fragment that contains two LXXLL-related motifs. The structure reveals critical contacts mediated through the first LXXLL motif of PGC-1α and the PPARγ coactivator binding site. Through a combination of biochemical and structural studies, we demonstrate that the first LXXLL motif is the most potent among all nuclear receptor coactivator motifs tested, and only this motif of the two LXXLL-related motifs in PGC-1α is capable of binding to PPARγ. Our studies reveal that the strong interaction of PGC-1α and PPARγ is mediated through both hydrophobic and specific polar interactions. Mutations within the context of the full-length PGC-1α indicate that the first PGC-1α motif is necessary and sufficient for PGC-1α to coactivate PPARγ in the presence or absence of rosiglitazone. These results provide a molecular basis for specific recruitment and functional interplay between PPARγ and PGC-1α in glucose homeostasis and adipocyte differentiation.
  Yong LI , Lin Zhang YANG and Chao WANG
  Field experiments were performed to identify an effective fertilizing scheme for direct-seeding rice (DSR) fields in the Taihu Lake Basin in east China. Based on local traditions, 3 typical fertilizing schemes (FS-1, FS-2, and FS-3) were evaluated, in consideration of ensuring a certain rice yield and relatively low nitrogen (N) loss. The base, seedling, tillering, jointing, and panicle fertilizers for FS-1 were all 20% of 270 kg N ha-1, those for FS-2 were 30%, 30%, 0%, 25%, and 15% of 270 kg N ha-1, and 15%, 20%, 25%, 20%, and 20% of 220 kg N ha-1 for FS-3, respectively. The results show that the majority of fertilizer N for DSR should be applied as topdressing fertilizer and not as base fertilizer as in transplanted rice cultivation. Increasing base fertilizer would not significantly improve the growth or yield of rice due to the low uptake of N during the seedling stage, and in turn would lead to greater N loss. Under FS-1, FS-2, and FS-3, N loss was 91.4, 103.1, and 70.5 kg ha-1, respectively, via surface runoff, volatilization, and leaching. Furthermore, using different fertilizer N methods during the rice growing season led to different N uptake by rice plants. In the present study N uptake by rice was measured at 108, 91, and 102 kg N ha-1 under FS-1, FS-2, and FS-3, respectively. At the same time, the rice yield with FS-1, FS-2, and FS-3 was 8530, 7780, and 8620 kg ha-1, respectively. In modern agricultural management an effective fertilizer scheme should simultaneously benefit both rice yield and the water environment. As a result, FS-3 was used in this study for DSR cultivation in the Taihu Lake Basin, which resulted in good rice yield and totally reducing N loss of 20.9-32.6 kg N ha-1.
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