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Articles by Yong Chen
Total Records ( 5 ) for Yong Chen
  Bing Han , Hui-Ling Zhang , Li Zeng , Bin Yang , Reyanggu Abula , Xiao-Li Xu and Yong Chen
  The beta-adrenergic receptors (β-AR) are a class of metabotropic G protein-coupled receptors that responsible for the muscle growth, fat deposition or lactation characteristics of domestic animals. The aim of this article was to scan nucleotide mutations in partial coding region of β2-AR gene on cattle. In this study, the polymorphisms of β2-AR gene on cattle were detected by methods of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and DNA sequencing in two cattle breeds in China, Xinjiang Brown cattle and Chinese Holstein cows. The results revealed that there were two genotypes and homozygote variant was not found in the two breeds. The genotypic frequencies at the Sma I locus were significantly different between the two breeds. The wild-type allele was ascendancy in the two breeds but its allele frequency was distinctly lower in the Chinese Holstein cows than that in the Xinjiang Brown cattle. DNA alignment results showed that three single nucleotide polymorphisms (SNPs), C11A, G53A and C129T were found in 5-coding region of β2-AR gene. The C11A mutation resulted in disappearance of Sma I cleavage site. In addition, the C11A and G53A mutations caused two amino acid residues replacement at the 4th and 18th of β2-AR. However, the C129T mutation was a synonymous one. In conclusion, the heterozygous frequency in Chinese Holstein cows was obviously higher than that in the Xinjiang Brown cattle. The Sma I locus would be a potential genetic marker for lactation performance of cattle.
  Yong Chen , Yong Zhao , Zhu-Yan Fu , Zhao-Wei Ma , Fa-Chen Qian , Anwaier Aibibuli , Bin Yang , Reyanggu Abula , Xiao-Li Xu and Azimaitijiang Aniwaer
  The aim of this study was to investigate nutritive value of tetraploid black locust (Robinia pseudoacacia). Chemical composition and in vitro fermentation characteristics by using in vitro gas production technique were stated for tetraploid black locust, birdsfoot trefoil (Lotus corniculatus L.), alfalfa (Medicago sativa cv.) and galega (Galega orientalis Lam.). Tetraploid black locust showed high crude protein (16.02%), ether extract (2.85%), crude ash (13.16%) and Ca (3.85%) content and low neutral detergent fibre (21.63%), acid detergent fibre (19.91%) and acid detergent lignin (6.52%) content. However, fermentation characteristics, such as cumulative gas production of 48 h, dry matter degradability, neutral detergent fibre digestibility, total volatile fatty acid of tetraploid black locust, were lower than that of birds foot trefoil and alfalfa (p<0.05). Tetraploid black locust fermentation resulted in reducing the molar proportions of acetate and butyrate and increasing that of propionate (p<0.05). In conclusion, according to the chemical composition, tetraploid black locust was a high-quality feed resources. However, utilization of tetraploid black locust by ruminal microorganisms in vitro was limited. Further in vivo studies could be required to expand the knowledge of the nutritive value of tetraploid black locust.
  Yong Chen , Huiling Zhang , Hongyong Wang and Kailun Yang
  This study was supposed to evaluate the effects of three non-ionic surfactants (NIS) (Tween 40, 60 and 80) on nutrient digestibility and rumen fermentation. Four Chinese Merino rams, fitted with ruminal and duodenal cannulas, were used in a 4x4 Latin square designed experiment. The four treatments were the basal diet (the control) and the basal diet supplemented with 10 g day-1 Tween 40, 60 or 80, respectively. Results showed that there were no differences among the four treatments in nutrient intakes. When compared to the control, feeding NIS had no effect on ruminal or total tract digestibility of Organic Matter (OM), Crude Protein (CP), cellulose and hemicellulose. Sheep consuming Tween 60 or 80 tended to have higher apparent ruminal digestion of Dry Matter (DM) (p = 0.074). On rumen fermentation characteristics, ruminal pH and protozoa counts were not affected by NIS supplementation. However, carboxymethylcellulase activity was improved by Tween 60 and 80 (p<0.01). Sheep feed Tween 40, 60 or 80 resulted in lower (p<0.05) ruminal ammonia nitrogen (NH3-N) and higher (p<0.01) total Volatile Fatty Acid (VFA) concentrations. In terms of individual VFA, including Tween 60 or 80 in the diet significantly improved concentrations of acetate (p<0.01), propionate (p<0.01) and butyrate (p<0.05) in ruminal fluid but did not alter the ratio of acetate to propionate. It was concluded that Tween 60 and 80 reduced rumen NH3-N concentration and increased carboxymethylcellulase activity and total VFA, as well as acetate, propionate and butyrate concentrations but had no effect on nutrient intakes and digestion. It is inferred that potential uses of the Tween 60 and 80 as feed additives for sheep to optimize ruminal fermentation.
  Shan-Wu Dong , Yong Chen , Guang-Hua Liu , Lin Chen , Xue-Hua Zhao and Zhenyu Wang
  Background and Objective: Cadmium is a heavy metal that accumulates with time in different tissues of the body like central nervous system, kidney, liver etc. causing toxic effects especially to the brain and has a very long biological half-life. The objective of this study was to explore the neuroprotective effect of cadambine an indole alkaloid against cadmium-induced toxicity. Materials and Methods: Three cell lines namely N2A, SH-SY5Y and primary brain neonatal rat cells were used in this study. Cell viability and neuroprotection assays were assessed by MTT assay to determine the effect of cadmium and cadambine. Changes in the intracellular calcium levels, ROS and mitochondrial membrane potential were determined by flow cytometer. Western blotting was done to analyse the effects of cadmium and cadambine on pro-apoptotic and anti-apoptotic genes. Student’s t-test was used to compare the data. Results: In this study, it was confirmed that cadmium induced toxicity was reversed by cadambine. Cadambine increased the viability of cells treated with of cadmium induced toxicity. Furthermore, cadmium treatment led to generation of ROS, increased calcium levels, loss of MMP and increase in the expression of pro-apoptotic proteins. Interestingly, these effects was reversed by cadambine in primary brain neonatal rat cells. Conclusion: These findings led us to the conclusion that exposure to cadmium induces neurotoxic effects in brain and cadambine not only reversed the toxic effects of cadmium but also led to inhibition of cadmium-induced apoptosis. Based on this data, cadambine can further be investigated as potent neuroprotective agent.
  Yong Chen , Xijiang Pan , Ying Tang , Shu Quan , Phang C. Tai and Sen-Fang Sui
  SecA is an obligatory component of the Escherichia coli general secretion pathway. However, the oligomeric structure of SecA and SecA conformational changes during translocation processes are still unclear. Here we obtained the three-dimensional structure of E. coli wild-type full-length SecA in solution by single particle cryo-electron microscopy and determined its oligomeric organization. In this structure, SecA occurs as a dimer in which the two protomers are arranged in an antiparallel mode, with a novel electrostatic interface, and both protomers are in closed conformation. The system developed here may provide a promising technique for studying dynamic structural changes in SecA.
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