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Articles by Ying Liu
Total Records ( 14 ) for Ying Liu
  Shiping Zhu , Ying Liu , Wenhua Dong , Xianrui Zheng , Guoqiang Zhu , Shenglong Wu and Wenbin Bao
  To assess the feasibility of FUT1 gene M307 as a disease resistance genetic marker in Yorkshire pigs, this experiment adopted PCR-RFLP method to investigate the genetic variations at M307 of FUT1 gene in purebred Yorkshire (total 218 individuals), determined partial biochemical parameters and important cytokines using the automatic blood cell analyzer and ProCarta immunoassay kits and assayed a number of economic traits including eye muscle thickness, backfat thickness, growth rate and reproductive performance. This study also analyzed the relationship between polymorphism of FUT1 gene M307 and partial immune indexes, vital economic traits. The results showed that there were three genotypes (AA, AG and GG) in Yorkshire test groups, in which the allele G was dominant one. FUT1 gene M307 deviated from Hardy-Weinberg equilibrium (p<0.01) in the population. Correlation analysis showed that the level of thrombocytocrit (PCT) in AG, GG genotype individuals were significantly higher than that in AA genotype individuals (p<0.05), while there were no significant difference between AG and GG genotypes (p>0.05). The individuals with GG genotype were significantly higher than those with AG genotype (p<0.01) in IFN-γ, TGF-β and TNF-α. In terms of the age at 100 kg, individuals with AA type were significantly higher than those with AG type (p<0.05), the average age at 100 kg of AA type individuals was 4.23 days less than GG type individuals. From the 3rd-5th parity, the Total Number Born (TNB) and Number Born Alive (NBA) demonstrated a trend of AA>AG>GG, individuals with AA type were significantly higher than those individuals with GG type (p<0.05) in TNB and NBA at 3rd and 5th parities. These results indicated that AA genotype, as an ETEC F18 resistance genetype, would also improve growth rate and reproduction performance of Yorkshire pigs, but reduce their general disease resistance ability.
  Yan Liang , Bin Fang , Jiye Qian , Lin Chen , Chunyan Li and Ying Liu

The identification of non-cell objects in biological images is not a trivial task largely due to the difficulty in describing their characteristics in recognition systems. In order to better reduce the false positive rate caused by the presence of non-cell particles, we propose a novel approach using a local jet context features scheme combined with a two-tier object classification system. The newly proposed feature scheme, namely local jet context feature, integrates part of global features with the “local jet” features. The scheme aims to effectively describe the particle characteristics that are invariant to shift and rotation, and hence help to retain the critical shape information. The proposed two-tier particle classification strategy consists of a pre-recognition stage first and later a further filtering phase. Using the local jet context features coupled with a multi-class SVM classifier, the pre-recognition stage intends to assign the particles to their corresponding classes as many as possible. To further reduce the false positive particles, next a decision tree classifier based on shape-centered features is applied. Our experimental study shows that through the proposed two-tier classification strategy, we are able to achieve 85% of identification accuracy and 80% of F1 value in urinary particle recognition. The experiment results demonstrate that the proposed local jet context features are capable to discriminate particles in terms of shape and texture characteristics. Overall, the two-tier classification stage is found to be effective in reducing the false positive rate caused by non-cell particles.

  Ying Liu and Hong-bo Bi
  The accuracy of dynamic prediction of productivity influences the reasonableness of the selected development program greatly. The characteristics and applicable conditions of oil-water composite method, fluid productivity index method and Petrobras method are analyzed in this study. Afterwards, we propose to combine three methods mentioned above to derive a novel productivity analysis model. We calculate the fluid productivity index firstly and then calculate the unknown set of test points based on the principle of Petrobras method, finally choose oil-water composite method to draw the IPR curve. In this study, after calculating reservoir pressure and saturation pressure, we exploit the proposed model to design a productivity analysis and forecasting system for single well. The results show that compared with the traditional methods, the proposed method improves the productivity forecasting accuracy.
  Faxiang Tao , Ying Liu and Ning An
  α-cellulose is widely used as a target substance for isotope ratio analysis in environmental reconstructions. Its preparation includes three basic steps: organic solvent extraction, delignification and alkaline hydrolysis. Recent works have suggested omission of the first step. We have made a detailed comparison in carbon isotope ratio of α-cellulose with or without organic solvent extraction using 32 consecutive tree ring and 30 subfossil peat samples. These samples were exposed to three different chemical treatments: with organic solvent extraction as the first step (CellOE), without organic solvent extraction (CellNOE), and with organic solvent extraction as the final step (CellNOE/OE). The third treatment is used to test if organic extractives can be completely removed or if their solubility in organic solvents has been altered by delignification and alkaline hydrolysis. In tree rings and peat, δ13CCell NOE was always significantly different from δ13CCell OE, but the trends were not the same. In tree rings, δ13CCell NOE was always more negative than δ13CCell OE by -0.31 ~ -0.01‰. In contrast, δ13CCell NOE in peat could be more negative or more positive than δ13CCell OE by -3.08 ~ 0.27‰. The third chemical treatment resulted in different patterns. For tree rings, δ13CCell NOE/OE was still more negative than δ13CCell OE by -0.36 ~ -0.08‰. However, the differences between δ13CCell NOE/OE and δ13CCell OE for peat varied in a more narrow range from -0.58 to 0.61‰, compared to the differences between δ13CCell NOE and δ13CCell OE. These results exposed a complex chemical evolution behaviour and an incomplete removal of lipids during delignification and alkaline hydrolysis. The mean value, long-term trend and seesaw patterns for a tree ring or peat CellNOE series were significantly different from those for a CellOE series, indicating that omission of organic solvent extraction will lead to a biased inference of past environmental conditions.
  Li-Chun Zhao , Ying Liu , Zi Wang , Nong Tang , Jing Leng , Bing Zheng , Ying-Ying Liu and Wei Li
  Background and Objective: Platycodi Radix (PR), a famous traditional medicine and folk food, has been used in China. PR exerted numerous pharmacological activities including anti-tumor, anti-obesity and anti-inflammation. The present study aimed to investigate the active constituents by Liquid Chromatography/Mass Spectrometry (LC/MS) analysis and protective effects of saponins from the steamed Platycodi Radix (SSPR) on acute alcohol-induced liver injury in mice. Materials and Methods: Pretreatment with SSPR (100, 200 and 400 mg kg–1) prior to alcohol administration significantly prevented the activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG) in serum, malondialdehyde (MDA) level in liver tissue compared with the alcohol group (p<0.05). Results: The level of enzymatic antioxidants glutathione (GSH) was increased noticeably (p<0.05) in SSPR pretreatment mice liver tissue. Histopathological examination revealed that pretreatment with 400 mg kg–1 of SSPR markedly ameliorated acute alcohol-induced hepatocyte apoptosis and fatty degeneration. The findings from LC/MS analysis indicated that SSPR had more saponins than unheated-processed one and SSPR mainly contain a polyacetylene (Lobetyolin) and 22 saponins. Conclusion: These results showed the beneficial effect of SSPR on acute alcohol-induced oxidative stress and liver damage.
  Shu-quan Xin , Zi Wang , Wei-Nan Hao , Xiao-Tong Yan , Qi Xu , Ying Liu , Wei Liu , Yan-Fei Li , Xin-Dian Li and Wei Li
  Background and Objective: The roots of Codonopsis lanceolata have been used in traditional medicine for treatment of many diseases in China. Till now, there is no evidence on the liver protection effect of C. lanceolata on alcohol-induced liver injury. The purpose of this study was to investigate the hepatoprotective effect of steamed C. lanceolata (SCL) on ethanol induced liver injury in mice. Materials and Methods: Experimental mice were pretreated with different doses of SCL (100-400 mg kg1) for 2 weeks by gavage feeding. Biochemical markers and enzymatic antioxidants from serum, liver tissue were determined. Results: The results showed that the activities of ALT, AST and TG in serum, MDA level in liver tissue, decreased significantly (p<0.05) in the SCL-treated group compared with the alcohol group. On the contrary, the GSH level was increased markedly (p<0.05). Histopathological examination revealed that SCL (400 mg kg1) pre-treatment noticeably prevented alcohol-induced hepatocyte apoptosis and fatty degeneration. Moreover, LC-MS/MS analysis of SCL showed that it mainly contain Lobetyolin, Tangshenoside, Lancemasides F, Lancemasides B (Lancemasides E, Codonolaside), Lancemasides G, Lancemasides A (Codonoposide, Codonolaside) and Codonoposide. Conclusion: These results provide the evidence on possible application of SCL on acute alcohol-induced oxidative stress and liver damage in clinic.
  Wen Yang , Ying Liu , Ganglin Xue , Huaiming Hu , Feng Fu and Jiwu Wang
  A hybrid inorganic-organic assembly, NaH3(C6H5NO2)4[GeW12O40] · 7H2O (1), has been synthesized and characterized by IR, UV, 1H NMR and single crystal X-ray diffraction. Crystal data: orthorhombic, P212121, a = 12.819(2) Aring, b = 19.326(3) Aring, c = 24.164(4) Aring, V = 5986.4(18) Aring3, Z = 4, R1 = 0.0541 (I > 2σ). The structure of 1 is based on a 1D zigzag infinite chain assembled by alternating [GeW12O40]4- and sodium-pyridine-2-carboxylic complex [Na(C6H5NO2)3(H2O)2]+ via W-Od-Na-Od-W.
  Daniel Stone , Ying Liu , Zong-Yi Li , Robert Strauss , Eric E. Finn , James M. Allen , Jeffrey S. Chamberlain and Andre Lieber
  Recombinant adeno-associated virus vectors based on serotype 6 (rAAV6) efficiently transduce skeletal muscle after intravenous administration and have shown efficacy in the mdx model of muscular dystrophy. As a prelude to future clinical studies, we investigated the biodistribution and safety profile of rAAV6 in mice. Although it was present in all organs tested, rAAV6 was sequestered mainly in the liver and spleen. rAAV6 had a minimal effect on circulating blood cells and caused no apparent hepatotoxicity or coagulation activation. rAAV6 caused some neutrophil infiltration into the liver, with a transient elevation in cytokine and chemokine transcription/secretion. In summary, rAAV6 induces transient toxicity that subsides almost completely within 72 h and causes no significant side effects.
  Hongjie Wang , Ying Liu , ZongYi Li , Sebastian Tuve , Daniel Stone , Oleksandr Kalyushniy , Dmitry Shayakhmetov , Christophe L. M. Verlinde , Thilo Stehle , John McVey , Andrew Baker , Kah- Whye Peng , Steve Roffler and Andre Lieber
  Gene transfer vectors containing adenovirus (Ad) serotype 35 (Ad35) fibers have shown promise for cancer and stem cell gene therapy. In this study, we attempted to improve the in vitro and in vivo infection properties of these vectors by increasing their affinity to the Ad35 fiber receptor CD46. We constructed Ad vectors containing either the wild-type Ad35 fiber knob (Ad5/35) or Ad35 knob mutants with 4-fold- and 60-fold-higher affinity to CD46 (Ad5/35+ and Ad5/35++, respectively). In in vitro studies with cell lines, the higher affinities of Ad5/35+ and Ad5/35++ to CD46 did not translate into correspondingly higher transduction efficiencies, regardless of the CD46 receptor density present on cells. However, in vivo, in a mouse model with preestablished CD46high liver metastases, intravenous injection of Ad5/35++ resulted in more-efficient tumor cell transduction. We conclude that Ad5/35 vectors with increased affinity to CD46 have an advantage in competing with non-CD46-mediated sequestration of vector particles after intravenous injection.
  Yong-Ming Tang , Wen-Zhong Yang , Xiao-Shuang Yin , Ying Liu , Rong Wan and Jin-Tang Wang
  Three thiadiazole derivatives, namely 5-phenyl-2-amino-1,3,4-thiadiazloe (APT), 5-(4-methoxyphenyl)-2-amino-1,3,4-thiadiazloe (AMPT) and 5-(4-nitrophenyl)-2-amino-1,3,4-thiadiazloe (ANPT), have been synthesized as new inhibitors for the copper corrosion in 0.5 M H2SO4. The inhibition properties of the inhibitors were studied by means of potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) measurements. The results show that the order of inhibition efficiency is AMPT > APT > ANPT. The values of free energies of adsorption, as calculated from the Langmuir adsorption isotherm, indicate that the thiadiazole compounds adsorb on copper by a physisorption mechanism in 0.5 M H2SO4. The correlation between inhibition efficiency and quantum chemical parameters has been investigated by PM3 quantum chemical calculation.
  Yan-Nian Liu , Ying Liu , Han-Jung Lee , Yung-Hsiang Hsu and Ji-Hshiung Chen
  The loss of E-cadherin gene expression can cause the dysfunction of the cell-cell junction to trigger tumor metastasis. Members of the Snail family of transcription factors are repressors of the expression of the E-cadherin gene. In this study, we showed that the activated androgen receptor (AR) is a novel repressor of E-cadherin gene expression and can promote metastasis. Our results demonstrated that the activated AR could bind to the E-cadherin promoter in vitro and in vivo. The activated AR and HDAC1 had synergistic effects in downregulating E-cadherin gene expression. Treating cells with the AR ligand, dihydrotestosterone (DHT), triggered the reduction of E-cadherin expression and induced changes in cell morphology from an epithelial-like to a mesenchymal-like appearance. When nonmetastatic breast cancer cells expressing cytoplasmic AR were transplanted into mice and the mice were treated with DHT, tumors were detected at metastatic sites, whereas no tumors were detected in transplanted mice without DHT treatment. Furthermore, clinical data from breast cancer patients with invasive ductal carcinomas showed high levels of AR expression in the nuclei and low levels of E-cadherin expression. These results suggest that, similarly to Snail and Twist, the activated AR can downregulate E-cadherin expression to promote the activation of epithelial-mesenchymal transition and tumor metastasis.
  Mei Han , Ying Liu , Bing Zhang , Jinping Qiao , Weidong Lu , Yanyan Zhu , Yongyan Wang and Changqi Zhao
  Context: The destabilization of β-amyloid (Aβ) peptide aggregates and the protection of functional cells are the attractive therapeutic strategies for Alzheimer’s disease (AD). Some active ingredients of Salvia miltiorrhiza f. alba C.Y.Wu & H.W.Li (Lamiaceae) (SM) have attracted increasing attention for the treatment of neurodegenerative diseases. Objective: Salvianic borneol ester (SBE) is a new compound based on SM formulas. The present study was designed to examine the anti-amyloid effects and neuroprotection of SBE in vitro. Materials and methods: The destabilizing effects of SBE and its related compounds (salvianic acid A and borneol) on preformed Aβ oligomers were measured by using fluorescence spectroscopy with thioflavin T (ThT) and the destabilizing effects of SBE were further confirmed visually by transmission electron microscopy (TEM). The neuroprotective effects of SBE against hydrogen peroxide (H2O2)-induced toxicity in human neuroblastoma cells (SH-SY5Y) and motor neuron hybridoma cells (VSC 4.1) were shown by MTT assay and morphological observation. Results: SBE showed the most significant destabilizing effect, though the mixture of salvianic acid A and borneol also destabilized Aβ1−40 oligomers. The destabilizing activity of salvianic acid A or borneol alone was not significant. SBE destabilized Aβ1−40 oligomers in dose- and time-dependent manners and the destabilizing effect could also be seen in the photographs of TEM. Furthermore, SBE could protect SH-SY5Y cells and VSC 4.1 cells against H2O2-induced toxicity in a dose-dependent manner. Discussion and conclusion: SBE had the bifunctional activities of anti-amyloid and neuroprotection. It may have therapeutic potential for AD and be an alternative lead compound for developing new drugs against AD.
  Guangzuo Luo , Albrecht Gruhler , Ying Liu , Ole N. Jensen and Robert C. Dickson
  Eisosomes are recently described fungal structures that play roles in the organization of the plasma membrane and endocytosis. Their major protein components are Pil1 and Lsp1, and previous studies showed that these proteins are phosphorylated by the sphingolipid long-chain base-activated Pkh1 and Pkh2 protein kinases in vitro. We show that Pkh1 and Pkh2 phosphorylate Pil1 and Lsp1 in vivo to produce species B, and that heat stress, which activates Pkh1 and Pkh2, generates a more highly phosphorylated species, C. Cells with low Pkh activity lack species B and C and contain abnormally organized eisosomes. To verify that Pil1 phosphorylation is essential for correct eisosome organization, phosphorylated serine and threonine residues were identified and changed to alanines. A variant Pil1 protein lacking five phosphorylation sites did not form eisosomes during log phase growth, indicating that phosphorylation is critical for eisosome organization. We also found that eisosomes are dynamic structures and disassemble when the Ypk protein kinases, which are activated by the sphingolipid-Pkh signaling pathway, are inactivated or when the sphingolipid signal is pharmacologically blocked with myriocin. We conclude that eisosome formation and turnover are regulated by the sphingolipid-Pkh1/2-Ypk1/2 signaling pathway. These data and previous data showing that endocytosis is regulated by the sphingolipid-Pkh1/2-Ypk1/2 signaling pathway suggest that Pkh1 and -2 respond to changes in membrane sphingolipids and transmit this information to eisosomes via Pil1 phosphorylation. Eisosomes then control endocytosis to align the composition and function of the plasma membrane to match demand.
  Ying Liu , Kathleen S. Matthews and Sarah E. Bondos
  During animal development, distinct tissues, organs, and appendages are specified through differential gene transcription by Hox transcription factors. However, the conserved Hox homeodomains bind DNA with high affinity yet low specificity. We have therefore explored the structure of the Drosophila melanogaster Hox protein Ultrabithorax and the impact of its nonhomeodomain regions on DNA binding properties. Computational and experimental approaches identified several conserved, intrinsically disordered regions outside the homeodomain of Ultrabithorax that impact DNA binding by the homeodomain. Full-length Ultrabithorax bound to target DNA 2.5-fold weaker than its isolated homeodomain. Using N-terminal and C-terminal deletion mutants, we demonstrate that the YPWM region and the disordered microexons (termed the I1 region) inhibit DNA binding ~2-fold, whereas the disordered I2 region inhibits homeodomain-DNA interaction a further ~40-fold. Binding is restored almost to homeodomain affinity by the mostly disordered N-terminal 174 amino acids (R region) in a length-dependent manner. Both the I2 and R regions contain portions of the activation domain, functionally linking DNA binding and transcription regulation. Given that (i) the I1 region and a portion of the R region alter homeodomain-DNA binding as a function of pH and (ii) an internal deletion within I1 increases Ultrabithorax-DNA affinity, I1 must directly impact homeodomain-DNA interaction energetics. However, I2 appears to indirectly affect DNA binding in a manner countered by the N terminus. The amino acid sequences of I2 and much of the I1 and R regions vary significantly among Ultrabithorax orthologues, potentially diversifying Hox-DNA interactions.
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