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Articles by Ying Zhu
Total Records ( 2 ) for Ying Zhu
  Weiqi Zeng , Yun Deng , Zhiyuan Yang , Wuzhou Yuan , Wen Huang , Chuanbin Zhu , Yan Bai , Yongqing Li , Zhonglu Peng , Yuequn Wang , Ying Zhu , Mingyao Liu and Xiushan Wu
  The calcium chloride method is an efficient and convenient technique to prepare Escherichia coli competent cells. The transformation efficiency of the competent cells when prepared in the laboratory is generally below 106 cfu μg-1 (colony forming units μg-1) DNA and thus its application is limited in some extent. Here we report the utilization of a-SiNPs (amino modified Silica-Nanoparticles) as a help material to improve the transformation efficiency of E. coli competent cells that are prepared with calcium chloride method. Addition of a-SiNPs increases the transformation efficiency of E. coli to 107-108 from below 106 cfu μg-1 DNA in the laboratory.
  Chuanbing Zhu , Xiangli Ye , Yuejuan Zhang , Hong Yang , Fang Liu , Ying Zhu , Zhenyu Cai , Yuequn Wang , Yun Deng , Wuzhou Yuan , Yongqing Li and Xiushan Wu
  As a model animal for developmental biology, chick embryo is easy to control and observe during embryo development period and therefore it is widely used in the study of cardiac development. The application of proteomics has opened the door for large-scale studies to dissect both protein expression, regulation and function during chick heart developing stages. The proteomics study requires to quickly separate a large number of chick heart samples with the same developing stage. However, the traditional morphological standards based on Hamburger-Hamilton and Witschi stages are difficult to fulfill this requirement. Herein, we suppose a new standard for distinguishing chick heart morphology in different developing stages based on the relationship between the embryonic heart development and the amnion folding in chick. Based on this standard, we can accelerate the speed of embryonic heart sample separation and guarantee the quantity and quality of the sample more reliably.
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