Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by Yetti Marlida
Total Records ( 17 ) for Yetti Marlida
  R. F. Ramadhan , Wizna , Yetti Marlida and Mirzah
  Objective: The aim of this study was to find out the best combination of bovine blood and agri-industrial waste which fermented by Bacillus amyloliquefaciens with different fermentation time to increase the quality of blood mixtures such as protein content, fiber content, nitrogen retention, amino acid composition and enzyme activity. Methodology: Bovine blood and agri-industrial waste obtained from a slaughterhouse and traditional markets. The design used in this study was a Completely Randomized Design (CRD) factorial using three factors. Results: The results showed that there was a highly significant interaction (p<0.01) between blood concentrations, types of agricultural waste and fermentation time on crude protein, nitrogen retention and energy metabolism which the best results was mixtures blood (300/250 mL) and coconut pulp with fermentation time 120 h whereas, the crude protein content, nitrogen retention and enzyme activity (protease and mannanase) were 50.70 and 55.60%, protease 37.34 U g–1 and mannanase 0.992 U g–1, respectively. Meanwhile, the mixtures of blood (300/250 mL) and PKC had the best amino acid composition. Conclusion: The study can be concluded that the best results were mixture of blood meal (300 mL) with coconut pulp and fermentation time 120 h.
  Mirnawati , Yose Rizal , Yetti Marlida and I. Putu Kompiang
  An experiment was conducted for the evaluation of Palm Kernel Cake Fermented (PKCF) by Aspergillus niger as a substitute for soybean meal protein in the diet of broiler. The experiment used a Complete Randomize Design (CRD) with 6 treatments and 4 replications. The treatments were arranged as follows: 1) 0% basic ration (0% PKCF), 2) 20% substitution of soybean meal protein with PKCF, 3) 40% substitution of soybean meal protein with PKCF, 4) 60% substitution of soybean meal protein with PKCF, 5) 80% substitution of soybean meal with PKCF, 6) 100% substitution of soybean meal with PKCF. The ration were formulated in iso protein 22% and iso caloric 3000 k cal/kg ration. This study used a completely randomized design with six treatments and four replications. The parameters of this study were feed consumption, body weight gained, feed conversion and percentage of carcass. The result of this study showed that feed consumption, body weight gained, feed conversion and percentage of carcase were not significantly affected (p>0.05) by any treatment. In conclusion the Palm Kernel Cake Fermented (PKCF) by Aspergillus niger can be 100% a substitute of soybean meal protein or 17% in broiler ration.
  Yetti Marlida , Nazamid Saari , Son Radu and Fatimah Abu Bakar
  The degradative activity on various raw starches by enzyme from Synnematous sp., an endophytic fungus was studied. The enzyme hydrolyzed raw starches to produce maltose and glucose. Maximum maltose and glucose were produced from raw rice and tapioca starch were 18.8 and 3.6%, respectively. The yields were dependent on accessibility of granules surface to be attacked by the enzyme. The results of this study suggest that enzyme from Synnematous sp. has potential for the production of glucose and maltose using raw starches as substrates.
  Mirnawati , Yose Rizal , Yetti Marlida and I. Putu Kompiang
  An experiment was conducted to improve the nutrient content of palm kernel cake through fermentation by Aspergillus niger with combination humid acid dosage and fermented time. The experiment used Complete Randomize Design (CRD) with 3 x 3 factorial and twice replication. The first factor was humic acid dosage: (1) 0 ppm, (2) 100 ppm and (3) 200 ppm. The second factor was fermented time: (1) 5 day, (2) 7 day and (3) 9 day. The parameters were dry matter, crude protein and crude fiber. The result of study showed that there was no significantly (p>0.05) interaction between humic acid dosage and fermented time to dry matter, but against crude protein and crude fiber, there were highly significant interaction (p<0.01). Humic acid dosage were not (p>0.05) effected to dry matter, but against crude protein and crude fiber, there were significant (p<0.01) effect. Fermented time showed that there were highly significant (p<0.01) effect to dry matter, crude protein and crude fiber. The conclusion was palm kernel cake which was fermented by Aspergilus niger showed that humic acid 100 ppm and fermented time 7 day had a better content. This condition can be seen in crude protein 23,20%, crude fiber 10,59% and dry matter 42.38%.
  Yetti Marlida , Rina Delfita , Peri Adnadi and Gita Ciptaan
  Thirty four isolates of endophytic fungus produce phytases were isolated from leaf, stem and root fragments of soybean. Two isolates were the best of phytases enzyme producer and identified as Rhizoctonia sp. and Fusarium verticillioides. The phytase production was induced by phytate in medium used. The crude preparations were used in subsequent characterization studies, pH and temperature optimum and compared to other phytases tested and is thus a promising candidate for animal feed applications. The results showed that optimal production of phytase from Rhizoctonia sp. were pH 4.0 and temperature 50oC and pH 5.0, temperature 50oC for Fusarium verticillioides.
  Arnim , Ferawati and Yetti Marlida
  The aim of this research was to test the effectiveness of coconut shell liquid smoke in preservation of meatballs. The research had been performed using factorial Completely Randomized Design (CRD) with two factors i.e. liquid smoke concentration (0%, 3%, 5% and 7%) and shelf life at refrigeration temperature (0, 5, 10 and 15 days). Parameter analyzed were water content, protein content, fat content, pH and Total Plate Count (TPC). Liquid smoke concentration had significant effect on water content, protein content, fat content, pH and Total Plate Count. Shelf life had significant effect on water content, protein content, fat content and Total Plate Count but not for pH. The interaction of liquid smoke concentration and shelf life had significant effect on protein content only. Applications of 7% liquid smoke in meatballs at 4±1°C increased shelf life until 15 days storage were better accepted and retarded the decreased of pH and moisture content compared to control. The result indicated that liquid smoke was an effective preservative agent for meatballs.
  Gita Ciptaan , Yetti Marlida , Periadnadi and Yose Rizal
  The research was conducted to determine the optimum dose of phytase supplementation of Fusarium verticillioides on Phosphor-deficient rations. This study used 24 broiler chickens (4 weeks). This research used Completely Randomized Design (CRD) with five ration treatments with 4 replications. Ration treatment was supplemented phytase with different doses: R1: 0 U/kg, R2: 250 U/ kg, R3: 500 U/kg, R4: 750 U/kg and R5: 1000 U/kg. Ration was based on iso protein (20%) and iso energy (2882 kcal/kg). The variables measured were retention of phosphor, calcium and nitrogen. The results showed that supplementation of phytase on broiler rations which Phosphor-deficiency was influenced the retention of Phosphor, calcium and nitrogen significantly (P<0.01). The optimum phytase supplementation of Fusarium verticillioides on broiler rations which phosphor-deficient was 750 U/kg ratio, it is seen from retention of phosphor (71.38%), calcium (75.65%) and nitrogen (67.61%).
  Harnentis , Yetti Marlida , Yose Rizal and Maria Endo Mahata
  Eleven isolates of thermophilic bacteria were isolated from hot spring and collected from West Sumatera of Indonesia. The bacteri isolated were grown on medium agar containing locust bean gum at 60°C for 48 hours to examine their mannanase production by using Congo red test. It was found that four isolates showed positive results with clear zone around the cultures. All isolates were evaluated their mannanase activity by growing in liquid medium supplemented with 0.5% locust bean gum as mannan source. It was found that isolate SM-1.4 displayed that highest enzyme activity of 119,44 U/ml and specific activity of 19.55 U/mg protein. The optimal growth conditions were at 60°C, pH 7 and 24 hours of incubation. According to the morphological studies, the isolate SM-1.4 was primarily identified as the genus Bacillus. The bacteria was gram negative and has endospore.
  Ucop Haroen , Yetti Marlida , Mirzah and Agus Budianyah
  Antibacterial activity limonoid compounds of three different solvent extracts (methanol, ethyl acetate and n-heksane) prepared by soxhlet extractor from orange waste juice were screened against two pathogenic bacteria Escherichia coli and Salmonella enteridis. The highest antibacterial potentiality was exhibited by the limonoid-ethyl acetate followed by the (limonoid-methanol) and (limonoid-n-hexane).The orange waste juice extract can be considered to be as equally potent as the antibiotics. The zone of inhibition, Minimum Inhibitory Concentration (MIC) of the orange waste juice extracts showed that limonoids extracted with ethyl acetate (limonoids-ethyl acetate) resulted in better inhibition of limonoids extracted with (limonoids-methanol) and (limonoids-n-hexane). The concentration 250 ppm of MIC were test showed inhibition of (limonoids-ethyl acetate) higher with a percentage minimum (44.15 for the bacterium Escherichia coli and 14.69 for the salmonella enteridis) than (limonoids-methanol) (10.67 and 3.84) and (limonoids-n-hexane) (35.58 and 14.10). In vitro antibacterial screening of crude (limonoid-ethyl acetate) (limonoid-methanol) and (limonoid-n-hexane) of methanol extract of orange juice waste with concentration 250 ppm were test showed the clear zone in millimeters (11.75 for the bacterium Escherichia coli and 10.25 for the salmonella enteridis) than limonoid-methanol (8.00 and 9.00) and limonoid-n-hexane (8.00 and 9.25). However, when compared to synthetic antibiotics (neomiditril and coleridin), the clear zone of limonoids lower (24%) but still showed his ability as an anti-microbial. (Limonoids-ethyl acetate) dose of 250 ppm showed better activity than doses of (limonoid-methanol) and (limonoid-n-heksana) for the bacterium Escherichia coli to salmonella enteridis bacterial at 11.918 and 9.158. From these results we can conclude (limonoids-ethyl acetate) extract better than (limonoids-methanol) and (limonoids-n-hexane) and can be used as a substitute for anti biotic synthetic antimicrobial. The phytochemical analysis of orange waste juice extracts showed presence of triter penes, alkaloid, flavonoids, steroid, phenolic, saponin.
  Yunilas , Lili Warli , Yetti Marlida and Irsan Riyanto
  This study aimed to test the ability of indigenous bacteria derived from oil palm waste to degrade lignocellulose as sources of inoculum fermented to high fibre feed. The selection is based on the highest ratio of clear zone the colony grow for 24 h (Kluepfel, 1988). The study we got 10 isolates that could degrades lignocellulose, but 5 among it has a better ability to degrades lignocellulosa with the ratio of clear zone (index hydrolysis) there are YL.B1 (2.9), YL.B2 (2.7), YL.B7 (2.6), YL.B8 (1.7) and YL.B9 (2.5). YL.B1 is the best isolates to degrades lignocellulosa, it shown by the highest hydrolysis index value rather than other isolates. Isolates YL.B1 is indigenous bacteria from oil palm waste, it has the potential to degrade the fiber (lignocellulose) and can be used as a source of inoculum fermented for to high-fiber feed.
  Mirnawati , Ade Djulardi and Yetti Marlida
  An experiment was conducted to improve the nutrient content of palm kernel cake through fermentation by Eupenicillium javanicum with combination inoculums dosage and fermented time. The experiment used complete randomized design (CRD) with 3x3 factorial and twice replication. The first factor was inoculums dosage: (1) 4, (2) 7 and (3) 10%. The second factor was fermented time: (1) 7 days, (2) 11 days and (3) 15 days. The parameters were dry matter, crude protein, crude fiber and fat of palm kernel cake fermented. The result of study showed that there was highly significant (p<0.01) interaction between inoculums dosage and fermented time to dry matter, crude protein and crude fiber but crude fat was no interaction (p>0.05). Each factor, inoculums dosage and fermented time were significantly (p<0.05) affected to dry matter, crude protein, crude fiber and crude fat. It is concluded that palm kernel cake fermented by Eupenicillium javanicum showed inoculums dosage 10% and fermented time 11 days had a better nutrient content. This condition can be seen in dry matter (42.21%), crude protein 26.27% and crude fiber 11.37% of palm kernel cake fermented.
  Nurjama yah , Yetti Marlida , Arnim and Yuherman
  Rimbo Panti hot springs located bordering the province of West Sumatra and North Sumatra, precisely in the District of East Pasaman Pasaman regency of West Sumatra Province approximately 200km from the city of Padang. Hot water samples obtained from 5 pools which have a normal temperature of 50-95°C. The aim of this research was to isolate and identify Lactic Acid Bacteria (LAB) thermophilic activity from hot spring with pathogen bacteria (Escherichia coli O157: H7, Salmonella thypmurium and Listeria monocytogenes) followed identified by PCR. The bacteria isolated were growth on medium thermus cair and then deMan Rogosa and Sharpe (MRS) Agar supplement with CaCO3 1% and then performed purification by plate out on deMan Rogosa and Sharpe (MRS) Agar. It was found 23 isolates of LAB showed with clear zone around the culture and 5 isolates (N2, N4, N6, N9 and N12) has been antimicrobial activity against the growth of pathogenic bacteria. The results showed that isolates N6 had the highest antimicrobial activity against all bacteria test, with a range of inhibition zone 18-30 mm, gram positive, spore former coccus, non motility and catalase negative. LAB isolates that have the widest diameter of the clear zone continued to test the minimum inhibitory concentration (MIC). MIC values of isolates N6 supernatant against pathogenic bacteria Escherichia coli O157: H7 by 60 and 80% of the bacterial pathogen Salmonella thypmurium and 50% of the bacterial pathogen Listeria monocytogenes. Based on morphological examination and PCR analysis, the isolate N6 was primarily identified as Pediococcus pentosaceus strain A24 bacteria.
  Ucop Haroen , Yetti Marlida , Mirzah and Agus Budianyah
  This study was conducted to evaluate the effect of orange (Citrus sinensis) waste juice extracts (OWJE) on performance in broilers chickens. A total of two hundred and forty unsexed broiler chicks (Arbor Acres CP-707) were randomly allocated to six treatments groups given varying concentrations of OWJE in the drinking water for 35 d. 0, 250, 500, 750 and 1000 ppm OWJE: water supplemented with an antibiotic only was used as a control. The growth responses achieved by broilers from all groups complied with standards. However, supplementation with 1000 ppm OWJE in drinking water increased feed intake and body weight gain, thereby increasing the feed conversion ratio of both starter (days 1-21) and finisher (days 22-35) broilers, while 750 ppm OWJE promoted feed intake and weight gain in starters. These results indicate OWJE is a useful additive for promoting broilers chicken growth. Additional research is needed to improve the suitability of OWJE as a feed additive which promotes growth in poultry.
  Mairizal , Yetti Marlida , Mirzah and Fahmida Manin
  Background and Objective: Mannanase is a mannan-degrading enzyme that can be produced by microorganisms such as bacteria. Mannanase is widely used in the animal feed industry with the aim of improving the quality of feed ingredients containing high levels of mannan, such as palm kernel meal. This study aimed to isolate, characterize and produce mannanase from bacteria found in the hindgut of termites. Materials and Methods: Bacteria were isolated by culturing on solid media containing 0.5% locust bean gum for 24 h at 30°C. The mannanolytic index was then measured by the addition of 2% congo red, which was then washed with 1 M NaCl. Mannanase activity was measured by the dinitrosalicylic acid method, while the protein content was measured by the Bradford method. Results: A total of 8 isolates were obtained. The 3 isolates with the highest mannanolytic indices were selected (isolates V9, AZ and AD). Isolate V9 exhibited the highest mannanase activity of 29.50 U mL–1 after 88 h of incubation with a specific activity of 110.32 U mg–1. The activity of the mannanase was optimal at pH 8 and at an incubation temperature of 50°C. Based on 16S-DNA analysis, isolate V9 was identified as Bacillus cereus. Conclusion: Bacillus cereus, which is isolated from the hindgut of termites, has fairly high mannanase activity and has the potential to hydrolyse mannan in feed ingredients such as palm kernel meal.
  Vabera Maslami , Yetti Marlida , Mirnawati , Jamsari and Yuliaty Shafan Nur
  Background and Objective: Glutamate is a non-essential amino acid and it improves the perception of the taste umami and serves as a building block of protein and physiological functions of the body. Increased use of glutamate in animal feed causes glutamate to rise globally. The aim of this study was to obtain the optimal conditions for glutamate production by Lactobacillus plantarum VM. Materials and Methods: Lactobacillus plantarum VM (L. plantarum VM) is a lactic acid bacteria originating from Minangkabau fermented foods and produces glutamate. The increased production of glutamate from Lactobacillus plantarum VM can be achieved by improving the nutrition and the growth environment of the bacteria. This study was designed in the form of a laboratory experiment protocol and was repeated 3 times. The variables measured in this study were the medium pH, temperature, incubation time, carbon source and nitrogen source. Results: The results of this study showed an optimum 5.5 pH (161.519 mg L–1), incubation time (36 h), temperature (36°C) (350.001 mg L–1), 11% glucose (566,535 mg L–1) and 0.5% peptone (680.525 mg L–1). Conclusion: Optimization of the initial pH of the media, incubation time, temperature, source C and source N can increase glutamate production.
  Afriani , Arnim , Yetti Marlida and Yuherman
  Background and Objective: Proteases are important enzymes and have high economic value due to their wide applications in the food industry as a meat tenderizer. Protease use in the food industry necessitates an understanding of the capabilities and influencing factors of these enzymes to accelerate enzymatic reactions. This study aimed to isolate and characterize the proteases of lactic acid bacteria (LAB) from Bekasam. Methodology: The samples were obtained from the third, fifth, seventh, ninth and eleventh day of fermentation to isolate the proteolytic LAB. Characterization of proteases includes the incubation time, casein substrate concentration, optimum temperature and pH, metal ion contents and stability. The LAB with the highest protease activity is identified molecularly and isolated through 16S rDNA sequencing and phylogenetic analysis based on the Neighbor Joining method. Results: The results showed that the best isolate was BAF-715 because it had the highest protease activity (18.84 U mL–1) at 40 h of incubation. The optimum activity of this protease on a casein substrate at 2.5% occurred at an incubation temperature of 40°C at pH 7 and in the presence of Mg2+ and Mn2+ (5 mM) as activators. Based on molecular DNA identification, the BAF-715 isolate is determined to be Pediococcus pentosaceus. Conclusion: A protease produced by Pediococcus pentosaceus showed the highest proteolytic activity, making it the best protease for application as a beef tenderizer.
  Elihasridas , Mardiati Zain , Yetti Marlida and Andri
  Background and Objective: This study aimed to determine the energy and protein balance in male Simmental cattle when fed diets with different protein and energy levels. Methodology: The experimental design used a randomized block design (RBD) with a 2×3×3 factorial pattern. There were two factors, namely, Factor A, which was the level of dietary energy (65 or 70% TDN) and Factor B, which was the level of dietary protein (10, 12 or 14%). Each treatment was repeated 3 times. The diet consisted of ammoniated rice straw and a concentrate and was fed at a ratio of 40:60. The concentrate consisted of palm kernel cake, rice bran, cassava, mineral and urea. The measured variables were intake; the digestibility of dry matter (DM), organic matter (OM) and crude protein (CP), daily gain, urine allantoin, blood metabolites and feed efficiency. Results: There was no interactive effect between the protein and energy levels of the diet on nutrient intake (DM intake, OM intake and CP intake), weight gain, or efficiency (p<0.05). There was an interactive effect between the dietary protein and energy levels on nutrient digestibility (DM, OM and CP) and urine allantoin (p>0.01). Nutrient intake tended to decrease with increasing levels of energy and protein in the diet, while nutrient digestibility increased with increasing energy levels in the diet but did not significantly change with increasing protein levels in the diet. The average daily gain and feed efficiency improved with the increasing levels of energy in the diet. There was an interactive effect (p<0.05) between the energy and protein levels on the cholesterol and HDL levels. Cholesterol levels increased with increasing levels of protein in the ration. There was no interactive effect (p>0.05) between the protein and energy levels on LDL, total protein, glucose, albumin, globulin, calcium or urea. Conclusion: It can be concluded that treatment A2B2, namely, an energy level of 70% TDN and a protein level of 12%, could provide the best reproductive performance of Simmental cattle in tropical areas.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility