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Articles by Yanying Zhang
Total Records ( 4 ) for Yanying Zhang
  Qiumei Shi , Guisheng Gao , Yanying Zhang , Hua Xiang , Zengqiang Yuan and Hongxuan He
  In this study, the viral RNA was extracted from swine FMDV and then the fragment of VP1 was amplified with a primer pair by RT-PCR. The interest fragmen was inserted into pGEM-Teasy vector. There combinant plasmid was identified by restriction analysis and PCR. It was proved by DNA sequencing that the acquired recombinant contains complete VP1 gene. The homologie of the nucleotide sequence of VP1 gene were 95.9 and 96.2%, respectively comparing with that of strai O/JPN/00 and O-Tibet-99. Afterwards, the complete VP1 gene from the identified recombin an was amplifie with another primer pair containing BamHI and XhoI sites by PCR and digested it with BamHI and XhoI. The expression vector pET28a were digested by BamHI and XhoI, respectively. The target gene VP1 was subcloned into vector pET28a. Positive clones named as pET28a-VP1 with interest gene were identificated by restriction analysis, PCR and DNA sequencing. Then there combinant was transformed into Escherichia coli BL21 (DE3) for VP1 expression. The interest gene was induced to express in E. coli with IPTG. The bacteria containing pET28a-VP1 were collected at different time and subsequently were examined by SDS-PAGE and Western-blotting.
  Yanying Zhang , Qiumei Shi , Guisheng Gao , Hai Fang , Cuizhen Chen , Gaili Ren , Huoju Chang and Donglin Zhang
  The detections of the Locus of Enterocyte Effacement Pathogenicity Island (LEE) and High Pathogenicity island (HPI) in 26 E. coli samples from chickens were conducted by PCR. Ler and eaeA genes located in the core of LEE Virulence Island while irp2, fyuA and asn_RNA_intB genes in HPI Pathogenicity Island were all detected. At the same time, O serotype identification of 26 E. coli samples were conducted. The results showed that the positive rate of Ler gene in LEE Virulence Island was 3.85% (1/26) while eaeA was 3.85% (1/26). The rate of fyuA gene in HPI pathogenicity island was 42.3% (11/26) while irp2 was 73% (19/26), irp2+fyuA+ was 42.3% there was no asn_RNA_intB gene. The results of serotype identifications showed that 15 strains related to serotype O4, O91, O78, O107, O38, O111, O88, O53, O24, O9 and O11 except 11 strains. Among 15 strains, O91, O78 and O38 were dominant serotypes whose proportion were 20 (3/15), 20 (3/15) and 26.7% (4/15), respectively. The nucleotide sequence similarities were >95% for 26 strains isolated from chickens.
  Qiumei Shi , Yanying Zhang , QiuYue Wang , Guisheng Gao , Guangping Gao , Hai Fang , Donglin Zhang , Cuizhen Chen and Xiaomei Lv
  To explore the pathogenic mechanism of Salmonella, serotype was identified and enterotoxin gene stn was detected for 47 strains suspected Salmonella in the Eastern part of Hebei Province. According to the bacterial culture characteristic, physicochemical properties and analysis results of K-3401 semi automatic bacteria identification instrument and identification for another time by Chengdu Institute of Biological Products, enterotoxin gene stn was detected by PCR. The 17 strains of Salmonella gallinarum, 14 strains of Salmonella typhimurium, 4 strains of Salmonella pullorum 2 strains of Salmonella paratyphi A, 2 strains of Salmonella group BO, 5 strains of Salmonella bovismorbificans, 3 strains of Salmonella enteritidis were detected from 47 strains of chicken source of Salmonella. The 45 strains of stn gene were amplified successfully in all 47 strains. The carrying rate was 95.7%. Homology of stn gene of test strains of Salmonella was between 94 and 100%. Evolutionary tree display that different serotypes of Salmonella enterotoxin stn gene divided into 5 groups. There was no homology with other bacterium. Chicken source of Salmonella contained 7 kinds of serotypes in the eastern part of Hebei Province. Among them, Salmonella gallinarum was 36.2% (17/47), Salmonella typhimurium which was advantages serotypes was 29.8% (14/47). The carrying rate of enterotoxin gene stn which had relation with bacterial pathogenicity was 95.7% (45/47).
  Yanying Zhang , Qiumei Shi , Shuqin Cheng , Guisheng Gao , Hai Fang , Guangping Gao , Yuqin Liu , Cuizhen Chen and Qiang Wang
  A total of 54 samples including duodenum, small intestine contents, lymphonodi mesenterici and diarrhea feces were collected from pigs died of diarrhea in Hebei Province of China in 2009~2011. These samples were examined for the presence of E. coli and serotype identification. High pathogenicity island was detected from E. coli isolates. The isolation and identification of O serotype of E. coli were conducted by common method, fyuA and irp2 genes were detected using PCR. The 54 E. coli strains referred to ten serotypes, O38 was the dominant serotype whose proportion was 51.5% (17/33). The positive rate of fyuA gene was 24.4% (11/45), irp2 was 42.2% (19/45), irp2 and fruA was 13.3% (6/45).
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