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Articles by Yanwirasti
Total Records ( 6 ) for Yanwirasti
  Dessy Arisanty , Yanwirasti , Jamsari Jamsari , Wirsma A. Harahap and Daan Khambri
  Background and Objective: miR-10b is one of oncogenic miRNA first described in promoting metastases in Breast Cancer. The aim of the study was to investigate the expression of miR-10b and the expression of metastases-induced genes in BC (BC) and fibroadenoma (FA) in West Sumatra. Materials and Method: LNATM primer enhancer set was used to identify the miR-10b expression as a relative quantification. miR-16 was used as an endogenous control with a relative median expression of miR-10b at 2–ΔΔCt. The expression of metastases-related genes was performed by an absolute quantification method. Results: The statistical differences between miR-10b and the expression of genes were determined by t-student test to interpret the expression of BC and FA tissue (p<0.005). The expression of miR-10b in BC was lower than FA (endogenous control). Relative median of miR-10b expression in BC was 8.51 times lower than FA. Low expression of miR-10b in BC was associated with tissue grading. The expression of metastases-related gene; RhoC, TIMP2 and MMP2 were lower in BC than FA. miR-10b expression differed between BC and FA. Conclusion: RhoC, TIMP2 and MMP2 expression caused cancer cell whether to be metastasized or not. miR-10b is a potential marker to predict BC cell which start to be aggressive.
  Hendriati , Yanwirasti , Tjahjono Darminto Gondhowiardjo and Djong Hon Tjong
  Background and Objective: Pathogenesis of pterygium is not fully understood. CYP1A1 gene found to have role in carcinogen metabolisms and also detected in pterygium tissue. The purpose of this study was to assess the CYP1A1 gene polymorphisms in patients with inflammatory and non-inflammatory pterygium. Methods: The PCR was conducted on blood samples of 80 patients with pterygium which consisted of 40 inflammatory and 40 non-inflammatory pterygium. The rs 4646903 SNP genotyping T>C (m1) in the CYP1A1 gene was conducted using Restriction Fragment Length Polymorphisms-PCR (RFLP-PCR). The PCR products were cut with restriction enzyme MspI. Results of restriction were then electrophorised and then observed with GelDoc. Results: In inflammatory pterygium, 13 patients obtained TT alleles (wild type), 19 TC allele (heterozygous mutants) and 8 CC alleles (homozygous mutant). In non-inflammatory pterygium, 14 patients obtained TT allele (wild type), 18 TC allele (heterozygous mutant) and 8 CC alleles (homozygous mutant). Conclusion: The CYP1A1 gene polymorphisms may occur in pterygium patients either inflammatory or non-inflammatory. Polymorphisms occur in the form of heterozygous and homozygous mutant.
  Hasmiwati , Jamsari , Yanwirasti , Nuzulia Irawati and Dahelmi
  Aedes aegypti is the major vector of DHF virus in the tropical and subtropical. The DHF prevention depends on vector control because the vaccine is still in development. Microsatellite has become on effective marker to obtain information about genetic diversity and analyze the structure of genetic population. The aim of this study was to isolate and characterize A. aegypti microsatellite markers, the Dengue Hemorrhagic Fever (DHF) vector in West Sumatra. Sequences containing microsatellites were obtained by enrichment method. Stages of works were as follows: isolation of A. aegypti genomic DNA, restriction with enzymes, ligation with adapters and hybridization by using microsatellite motifs. Furthermore, the candidate fragment contained motifs cloned on plasmid pGEM T easy vector using E. coli DH 5α with blue-white colony screening. The results showed 46 clones were successfully extracted from a total of 152 clones and became microsatellite motifs with repetition: (GA)3, (CTA)3, (GA)3 (TAAG)3, (ACTT)3 (TC)3 (AC)3. Eight pairs of primer were successfully designed from sequences containing microsatellite motifs with feasible flanking regions. The primer evaluation used 32 DNA samples of A. aegypti from 8 cities (population) in West Sumatra. These markers have been successfully amplified 9-17 alleles with amplification products ranging from 129-306 bp, with a high degree of polymorphism. Aedes aegypti microsatellite markers obtained can be used to analyze the structure of genetic population of A. aegypti and the obtained results were the additional microsatellite markers type of A. aegypti than what had previously existed.
  Arni Amir , Yanwirasti , Asmarinah and Fadil Oenzil
  One of factors causing oligozoospermic circumstances is excessive apoptosis during spermatogenesis. Spermatogenesis known involves Bcl-2 family proteins in cytoplasm and Voltage Dependent Anion Channel 1 (VDAC1) in outer mitochondrial membrane to facilitate releasing of apoptosis factor such as cytochrome-c from inter-membrane space into cytoplasm. The study was aimed to analyze the mRNA expression of pro-apoptotic Bax, anti-apoptotic Bcl-2 and VDAC1 genes derived from 45 oligozoospermic subjects and 20 fertile subjects as control. Analysis of transcript expression was performed by two-steps real-time (PCR) and calculating by standard curve method. Stages of works were followed: Analysis of sperm basal characterization, isolation of spermatozoa to separate it from cement and resulted pellets. Pellets were saturated with PBS to obtain mRNA and reversed into cDNA. The cDNA were sequenced to investigate SNP of Bax, Bcl-2 and VDAC1 genes. Results showed that comparison of log mRNA copy number of Bax, Bcl-2 and VDAC1 genes for oligospemic and fertile subjects varied. The Bax, Bcl-2 and VDAC1 were significantly different between oligozoospermic and normozoospermic subjects (p = 0.000, p = 0.041, p = 0.000, respectively). It was suggested that oligozoospermia may be occurred by inducing the increase of Bax pro-apoptotic and VDAC1 genes expression and decreasing of Bcl-2 expression to lead the excessive of apoptosis.
  Gusti Revilla , Eryati Darwin , Yanwirasti and Fedik A. Rantam
  Background and Objective: Burn is a public health problem, it causes physical disability even death. Treatment of burn wound has been conducted in various ways, but the satisfactory healing has not been provided. Bone marrow-derived mesenchymal stem cells (BM-MSCs) treatment is one of attempt to burn recovery, accelerate wound healing and angiogenesis. This study aimed to investigate the effect of allogeneic BM-MSC treatment on the expression of collagen type I and integrin α2β1 in burn skin tissue of rat observed on day 14. Materials and Methods: Twelve Wistar rats divided into two groups, control group (injected with phospate buffer solution) and treatment group (injected with BM-MSC). Rat was anaesthetized with xylazine and ketamine (ratio 1:1), fur of rat’s back was shaved and full thickness burn was made by boiling plate in hot water for 30 min and patched on the back for 20 min. The burns were covered by tegaderm film and elastomult haft. Antalgin as an analgetic was injected to rats during observation process. Burns of rat was observed on day 14. In this study one-way analysis of variance test and Tukey as a further test were analyzed. Results: The results showed that the healing time of allogeneic BM-MSC treatment on burn skin tissue rats was faster, the thickness of collagen type I in burn skin tissue of rats was thicker (0.977 μm) than controls (0.475 μm) and statistically demonstrated significant differences (p = 0.000). The average percentage of integrin α2β1 expression was higher (2.94%) than control group (2.34%), but the differences were not statistically significant (p = 0.176). Conclusion: The study concluded that BM-MSC treatment was able to accelerate the healing process of burns by increasing the thickness of the collagen and the percentage of integrin α2β1, thus accelerated the cell migration involved during wound healing.
  Yanwirasti , Wirsma A. Harahap and Dessy Arisanty
  Background: Abnormal expression of several microRNAs (miRNAs) has been demonstrated in many types of cancer tumor tissue. The miR-10b and miR-21 are an oncogenic miRNAs which play role in proliferation and invasion of Breast Cancer (BC) tumorigenesis. The aim of this study was to evaluate the miR-21 and miR-10b expression in BC in West Sumatran women, Indonesia. Materials and Methods: A total of 40 samples, consisting of 30 samples of breast cancer tissues (BC) and 10 samples of fibroadenoma tissues (FATs) as control and non-cancerous were analyzed. The miR-21 and miR-10b expression of each sample were investigated by using realtime PCR, followed by universal Reverse Transcription (RT) then real-time PCR amplification with specific primers. Hsa-miR-16-5p LNA PCR primer was used as an endogenous control. Results: The results showed that the expression level of miR-21 was more than 4 times higher in BC than in FATs. The expression level of miR-10b was lower in BC than FATs, by a factor of 3.34 fold. Both these differences were statistically different (p = 0.001). Conclusion: In this study it was concluded that for this sample of West Sumatran Women miR-21 expression in BC was higher than in FATs, whereas miR-10b was lower in BC than in FATs.
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