|
|
| |
| Articles
by
Yanmin Zhou |
Total Records (
2 ) for
Yanmin Zhou |
|
 |
| |
|
| |
Ming Qian
,
Min Liu
,
Mengna Duan
,
Haiyang Zhang
,
Zhe Wu
and
Yanmin Zhou
|
| |
Substance P (SP) is known to play an important role in bone
mineralization. However, little is known about the potential of substance P
as an osteogenic inducer in human Adipose-Derived Stem Cells (hADSCs) in
vitro. Therefore, the aim of this study to investigate the effects of substance
P on proliferation and osteogenic differentiation of hADSCs in vitro.
Human Adipose-Derived Stem Cells (hADSCs) derived from wasted adipose tissue
in liposuction operation were cultured. Expression of the SP receptor (NK1)
was detected by RT-PCR. Effects of SP on proliferation and differentiation of
hADSCs were studied, moreover alkaline phosphatase activity was also assayed.
It was found that the NK1 receptor was expressed in hADSCs. SP stimulated the
proliferation of hADSCs in a concentration-dependent manner. Higher concentrations
(10G8 M) of SP increased alkaline phosphatase activity and enhanced
differentiated in hADSCs. NK1 receptors are expressed by hADSCs and SP stimulates
osteoblast differentiation and function in vitro. These findings strengthen
the knowledge about the role of SP in hADSCs. |
|
|
| |
|
| |
Qiujue Wu
,
Yuqin Wang
,
Zhanbin Wang
,
Yanmin Zhou
and
Tian Wang
|
| |
Acid modified clinoptilolite are usually used as adsorbents
for NH3, heavy metals and organic contaminants. Acid-modified clinoptilolite
was prepared using the method of ion exchange reaction at 80°C for 4 h and
used for bacteria and toxins adsorption which are significant for providing
mechanism for the adsorption of microorganisms in vitro. XRD, SEM, AFM,
BET and micropore volume were used to characterize parent clinoptilolite and
acid modified clinoptilolite. Cation Exchange Capacity (CEC) was used to determine
changes of adsorption ability before and after modification. The acid-modified
clinoptilolite showed the strong adsorptive in the rang of 20-25 mg g-1
when the initial bacterial suspension concentration was 1x108 Colony
Forming Units (CFU)/mL. The adsorption capacities of acid-modified clinoptilolite
for all bacterial suspension increased with an increase of initial bacterial
suspension concentration. An increase of adsorption capability of acid-modified
clinoptilolite was attributed to the BET and micropore volume. These results
indicated the competency of acid-modified clinoptilolite adsorbent for bacteria
adsorption. |
|
|
|
|
| |
|
