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Articles by Yang Li
Total Records ( 11 ) for Yang Li
  Yueyan Wu , Zaiping Pan , Yang Li , Qi Zou and Pingsha Huang
  In order to know the soil environment impact on grape quality at a vineyard in Ningbo, the heavy metal contents of the soils and irrigation water in this vineyard were analyzed annually with reference to the relevant national criteria and specifications. At the same period of time, two vineyards at different places were selected as control. This study showed that compared with the controls, the soil environment quality at this vineyard could meet the requirements issued by Standard of Nuisance-free Food: Environmental Condition for Fresh Grape Production. The comprehensive pollution index of study sites were from 0.211-0.267, that means the vineyard is in a safe condition. It is also found that the soil environmental quality at Site A is better than that at Site B.
  Hu Shufang , Cheng Kui , Yang Li and Zhang Yu
  Background and Objective: The diagnosis of type 2 diabetic nephropathy (T2DN) patients is important to prevent the long-term damaging effects of kidney loss in patients with diabetes. The aim of this study was to explore diagnostic values of Urinary RBP levels, NGAL, ZAG and urinary transferrin in early diagnosis of diabetic nephropathy. Materials and Methods: The total sample size was comprised of 400 patients, 300 subjects were of type 2 diabetes mellitus and 100 subjects were considered under the control subjects without any systemic illness. According the ratio of urine and albumin urine creatinine, subjects were further divided into 3 sub-categories: normoalbuminuria, microalbuminuria and macroalbuminuria group. Human ELISA kit had been used for the evaluation of Urine urinary RBP, NGAL, Adipokine zinc-alpha-2-glycoprotein and urinary transferring. Results: The level of u-NCR, u-RCR, u-ZCR, u-TCR levels in the macroalbuminuria and microalbuminuria were markedly and significantly high when compared with normoalbuminuria and control subjects. So, when the stages of diabetic nephropathy are going critical, the levels of all the biomarkers also increasing in their value. urinary transferrin and RBP have excellent diagnostic accuracy with AUC = 1-0.9. Conclusion: This study concluded that the evaluation of urinary RBP, NGAL, ZAG and urinary transferrin could be use as novel and accurate biomarker for the early diagnosis, clinical monitoring and progression of diabetic nephropathy patients.
  Weihua Li , Yang Li , Bohua Zhang , Xuan Dong , Zhizhong Cui and Peng Zhao
  To investigate the Reticuloendotheliosis virus infection status in large-scale breeder chicken farms of China, 563 serum samples from 4 great-grandparents breeder farms, 6557 serum samples from 8 grandparents breeder farms, 312 serum samples from parents breeder farms, totally 7432 serum samples were collected and examined using the Reticuloendotheliosis Virus Antibody Test kit. The results showed that REV-antibody positive rates of great-grandparents breeder flocks, grandparent breeder flocks and parents breeder flocks were 0-31, 0-47 and 4.23-42.50%, respectively. REV-antibody positive rate has close relationship with incidence of REV infection history and tumor complaints. This study suggests that REV infection is very common in China large-scale breeder chicken farms and the prevention of REV should be given more attention this is the largest scale serological survey for breeder chickens in China so far.
  Vesna Ivancic , Mitra Mastali , Neil Percy , Jeffrey Gornbein , Jane T. Babbitt , Yang Li , Elliot M. Landaw , David A. Bruckner , Bernard M. Churchill and David A. Haake
  We describe the first direct testing of the antimicrobial susceptibilities of bacterial pathogens in human clinical fluid samples by the use of ATP bioluminescence. We developed an ATP bioluminescence assay that eliminates somatic sources of ATP to selectively quantify the bacterial load in clinical urine specimens with a sensitivity of <1,000 CFU per milliliter. There was a log-log relationship between light emission and the numbers of CFU in clinical urine specimens. A clinical study was performed to evaluate the accuracy of the ATP bioluminescence assay for determination of the antimicrobial susceptibilities of uropathogens in clinical urine specimens tested in a blinded manner. ATP bioluminescent bacterial density quantitation was used to determine the inoculation volume in growth medium with and without antibiotics. After incubation at 37°C for 120 min, the ATP bioluminescence assay was repeated to evaluate the uropathogen response to antibiotics. The ability of the ATP bioluminescence assay to discriminate between antimicrobial susceptibility and resistance was determined by comparison of the results obtained by the ATP bioluminescence assay with the results obtained by standard clinical microbiology methods. Receiver operator characteristic curves were used to determine the optimal threshold for discriminating between susceptibility and resistance. Susceptibility and resistance were correctly predicted in 87% and 95% of cases, respectively, for an overall unweighted accuracy of 91%, when the results were stratified by antibiotic. For samples in which the pathogen was susceptible, the accuracy improved to 95% when the results for samples with less than a 25-fold increase in the amount of bacterial ATP in the medium without antibiotics were excluded. These data indicate that a rapid bioluminescent antimicrobial susceptibility assay may be useful for the management of urinary tract infections.
  Mitra Mastali , Jane T. Babbitt , Yang Li , Elliot M. Landaw , Vincent Gau , Bernard M. Churchill and David A. Haake
  We have previously demonstrated the clinical validity of the rapid detection of uropathogens by use of a DNA biosensor. This assay involves the hybridization of capture and detector probe pairs with bacterial 16S rRNA target molecules to form a DNA-RNA sandwich on the sensor surface. Horseradish peroxidase-conjugated antibody binds to the detector probe to enzymatically amplify the hybridization signal. These previous studies involved the hybridization of bacterial 16S rRNA target sequences with 35-mer oligonucleotide probe pairs at 65°C. Achievement of point-of-care technology will be greatly facilitated by ambient-temperature detection. The purpose of this study was to examine the effects of probe length and target location on signal intensity using hybridization temperatures of 20 to 25°C. Signal intensity was found to vary dramatically with hybridization location in the species-specific bulge region of 16S rRNA helix 18. Probe pairs of as short as 10 nucleotides in length were able to produce a significant electrochemical signal, and signal intensity was correlated with probe length for probes of 10 to 20 nucleotides in length. The sensitivity of the Escherichia coli-specific 15-mer probe pairs was approximately 330 cells. These shorter probes allowed differentiation of Klebsiella pneumoniae from Proteus mirabilis 16S rRNA target sequences differing by a single nucleotide. A panel of oligonucleotide probe pairs ranging from 11 to 23 nucleotides in length was able to distinguish among seven groups of urinary tract pathogens. In conclusion, we have developed short oligonucleotide probe pairs for the species-specific identification of uropathogens at ambient temperature by use of an electrochemical sensor.
  Peng Li , Yang Li , Lijie Hong , Yousi Chen and Mujie Yang
  Poly(dimethylaminoethyl methacrylate) and poly(glycidyl methacrylate) were simultaneously cross-linked to form a polyelectrolyte humidity sensitive film on an interdigitated gold electrode, which was further coated with a layer of soluble polyaniline (PANI). The composite so prepared was characterized by UV–vis spectroscopy and scanning electron microscopy. Its electrical response towards humidity was investigated at room temperature and compared with that of PANI and the polyelectrolyte separately. It was revealed that the composite showed an impedance as low as 1.8 x 105 Ω even at 0%RH. In contrast, the impedance of the polyelectrolyte at less than 20%RH was too high to be measured, and PANI showed very low humidity sensitivity. The low impedance, good sensitivity as characterized by a linear change in impedance from 1.8 x 105 to 9 x 103 Ω over 0–95%RH, fast response and small hysteresis, makes the composite a promising candidate for humidity sensors capable of detecting very low humidity.
  Chuanguang Qin , Yang Li , Ruijie Zhang , Weining Niu and Yan Ding
  Anthocyanin pigments in the fruit of mockstrawberry (Duchesnea indica Focke), were extracted with 0.1% HCl in ethanol, and the crude anthocyanin extract was purified by a C18 Sep-Pak cartridge open-column chromatography. High-performance liquid chromatography (HPLC) with photodiode array detection and mass spectrometry (MS) was applied for the isolation and composition analysis of anthocyanins in the fruit of mockstrawberry and their aglycones from acid hydrolysis. Three anthocyanins were found in the fruit of mockstrawberry and they were identified as cyanidin 3-O-rutinoside (61%), peonidin 3-O-rutinoside (34%), and petunidin 3-O-rutinoside (5%), respectively, by spectroscopic methods (UV-vis and MS). The two major anthocyanins were isolated by preparative HPLC, and their chemical structures were further characterised by H1 NMR. On the basis of chromatographic data, the total anthocyanin content was 205 mg g-1 of the fresh fruit of mockstrawberry.
  Mingyi Xie , Axel Mosig , Xiaodong Qi , Yang Li , Peter F. Stadler and Julian J.-L. Chen
  Telomerase extends chromosome ends by copying a short template sequence within its intrinsic RNA component. Telomerase RNA (TR) from different groups of species varies dramatically in sequence and size. We report here the bioinformatic identification, secondary structure comparison, and functional analysis of the smallest known vertebrate TRs from five teleost fishes. The teleost TRs (312-348 nucleotides) are significantly smaller than the cartilaginous fish TRs (478-559 nucleotides) and tetrapod TRs. This remarkable length reduction of teleost fish TRs correlates positively with the genome size, reflecting an unusual structural plasticity of TR during evolution. The teleost TR consists of a compact three-domain structure, lacking most of the sequences in regions that are variable in other vertebrate TR structures. The medaka and fugu TRs, when assembled with their telomerase reverse transcriptase (TERT) protein counterparts, reconstituted active and processive telomerase enzymes. Titration analysis of individual RNA domains suggests that the efficient assembly of the telomerase complex is influenced more by the telomerase reverse transcriptase (TERT) binding of the CR4-CR5 domain than the pseudoknot domain of TR. The remarkably small teleost fish TR further expands our understanding about the evolutionary divergence of vertebrate TR.
  Yang Li , Zhulun Wang , Noboru Furukawa , Patrick Escaron , Jennifer Weiszmann , Gary Lee , Michelle Lindstrom , Jinsong Liu , Xiaohong Liu , Haoda Xu , Olga Plotnikova , Vidya Prasad , Nigel Walker , R. Marc Learned and Jin- Long Chen
  The nuclear hormone receptor peroxisome proliferator-activated receptor γ (PPARγ) plays central roles in adipogenesis and glucose homeostasis and is the molecular target for the thiazolidinedione (TZD) class of antidiabetic drugs. Activation of PPARγ by TZDs improves insulin sensitivity; however, this is accompanied by the induction of several undesirable side effects. We have identified a novel synthetic PPARγ ligand, T2384, to explore the biological activities associated with occupying different regions of the receptor ligand-binding pocket. X-ray crystallography studies revealed that T2384 can adopt two distinct binding modes, which we have termed "U" and "S", interacting with the ligand-binding pocket of PPARγ primarily via hydrophobic contacts that are distinct from full agonists. The different binding modes occupied by T2384 induced distinct patterns of coregulatory protein interaction with PPARγ in vitro and displayed unique receptor function in cell-based activity assays. We speculate that these unique biochemical and cellular activities may be responsible for the novel in vivo profile observed in animals treated systemically with T2384. When administered to diabetic KKAy mice, T2384 rapidly improved insulin sensitivity in the absence of weight gain, hemodilution, and anemia characteristics of treatment with rosiglitazone (a TZD). Moreover, upon coadministration with rosiglitazone, T2384 was able to antagonize the side effects induced by rosiglitazone treatment alone while retaining robust effects on glucose disposal. These results are consistent with the hypothesis that interactions between ligands and specific regions of the receptor ligand-binding pocket might selectively trigger a subset of receptor-mediated biological responses leading to the improvement of insulin sensitivity, without eliciting less desirable responses associated with full activation of the receptor. We suggest that T2384 may represent a prototype for a novel class of PPARγ ligand and, furthermore, that molecules sharing some of these properties would be useful for treatment of type 2 diabetes.
  Yang Li , Deborah H. Anderson , Qiang Liu and Yan Zhou
  Influenza A virus infection activates the phosphatidylinositol 3-kinase (PI3K)/Akt pathway by binding influenza A virus NS1 protein to the p85β regulatory subunit of PI3K. In this study, we report that NS1 binds to the inter-SH2 (iSH2) domain of p85β. Mutational analyses on p85β iSH2 domain defined that Val-573 is the critical amino acid (AA) that mediates NS1 and p85β interaction. In reciprocal gain of function experiments with p85α, we demonstrated that mutation to Val at Met-582 leads to NS1 binding and increased PI3K activity. Molecular modeling based on our experimental results suggested that, in addition to the interaction interface between the NS1 SH3 binding motif 1 (AA 164-167) and p85β Val-573, AA 137-142 in NS1 might interact with p85β. Indeed, mutations of AA 141 and 142 in NS1 disrupted the interaction between NS1 and p85β. Mutant virus PR8-NS1-141/142 was not able to activate Akt phosphorylation. Furthermore, PI3K assays demonstrated that, in wild-type virus-infected cells, p85β-associated PI3K activity was increased significantly. In contrast, in the mutant virus-infected cells containing mutant NS1 unable to interact with p85β, the p85β-associated PI3K activity up-regulation was not seen, suggesting that PI3K up-regulation is dependent upon the interaction between NS1 and p85β. Competition experiments and the immunoprecipitation studies demonstrated that NS1, p85β, and p110 form a complex in cells. Finally, the mechanism by which binding of NS1 to p85β regulates PI3K activity was discussed based on a predicted structural model of NS1-p85-p110 complex.
  Xinle Wu , Bryan Lemon , XiaoFan Li , Jamila Gupte , Jennifer Weiszmann , Jennitte Stevens , Nessa Hawkins , Wenyan Shen , Richard Lindberg , Jin-Long Chen , Hui Tian and Yang Li
  FGF19 subfamily proteins (FGF19, FGF21, and FGF23) are unique members of fibroblast growth factors (FGFs) that regulate energy, bile acid, glucose, lipid, phosphate, and vitamin D homeostasis in an endocrine fashion. Their activities require the presence of α or βKlotho, two related single-pass transmembrane proteins, as co-receptors in relevant target tissues. We previously showed that FGF19 can bind to both α and βKlotho, whereas FGF21 and FGF23 can bind only to either βKlotho or αKlotho, respectively in vitro. To determine the mechanism regulating the binding and specificity among FGF19 subfamily members to Klotho family proteins, chimeric proteins between FGF19 subfamily members or chimeric proteins between Klotho family members were constructed to probe the interaction between those two families. Our results showed that a chimera of FGF19 with the FGF21 C-terminal tail interacts only with βKlotho and a chimera with the FGF23 C-terminal tail interacts only with αKlotho. FGF signaling assays also reflected the change of specificity we observed for the chimeras. These results identified the C-terminal tail of FGF19 as a region necessary for its recognition of Klotho family proteins. In addition, chimeras between α and βKlotho were also generated to probe the regions in Klotho proteins that are important for signaling by this FGF subfamily. Both FGF23 and FGF21 require intact α or βKlotho for signaling, respectively, whereas FGF19 can signal through a Klotho chimera consisting of the N terminus of αKlotho and the C terminus of βKlotho. Our results provide the first glimpse of the regions that regulate the binding specificity between this unique family of FGFs and their co-receptors.
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