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| Articles
by
Yang Gao |
Total Records (
6 ) for
Yang Gao |
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Xueping Guo
,
Ga Zhang
,
Wenya Li
,
Yang Gao
,
Hanlin Liao
and
Christian Coddet
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In this paper, tin-bronze/TiN and tin-bronze/quasicrystal (AlCuFeB) composite coatings were fabricated by cold spray process. Microstructure and microhardness of the prepared coatings were investigated. Ball-on-disc dry sliding wear tests were conducted in an ambient condition to examine the tribological behavior of the composite coatings. The results show that the microhardness and the density of composite coatings increase significantly compared to the pure tin-bronze coating. The friction coefficient of composite coating decreases when reinforcing particles were introduced. Furthermore, the bronze/quasicrystal composite coating has a lower friction coefficient and wear rate than the bronze/TiN coating. Tribological mechanisms of the composite coatings were discussed. |
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Yuelin Gao
,
Zaimin Ren
and
Yang Gao
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A modified differential evolution algorithm of constrained nonlinear mixed integer programming problems is given. In this algorithm, the positions of the variation particles are self-adaptively adjusted so that the particles evolve in better direction and the feasible basis rule and the dynamic constraint handling technology are added to improve particles’optimization ability. Numerical results show that the proposed algorithm is of high precision and stability. |
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Chengqing Guo
and
Yang Gao
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In this study, a class of coupled memristive neural networks of neutral-type
with stochastic perturbations via., pinning adaptive control in order to achieve
synchronization is studied. The analysis in this study employs the differential
inclusions theory, linear matrix inequalities and the lyapunov functional method
and some criteria are obtained to guarantee coupled memristive neural networks
of neutral-type with stochastic perturbations can achieve synchronization. Furthermore,
numerical examples are given to show the effectiveness of our results. |
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Ying Li
,
Yang Gao
,
Yujuan Zhang
and
Youjin Hao
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Fat cells in adipose tissue turn over throughout the life span. The objective
of this study was to explore the cellular and molecular basis for regional and
body weight differences in grass carp (Ctenopharyngodon idella) preadipocyte.
The number of fresh isolated preadipocytes were counted from different body
weight and anatomical sites using the hemocytometer and the proliferation capacity
of the cells was tested by MTT assay. In addition, the differentiation capacity
of cells was assessed through mRNA expression of Peroxisome Proliferator-Activated
Receptor-γ ( (PPAR-γ) and cellular Triglycerides (TG) content. The
total number and proliferative capacity of visceral cells was significantly
(p<0.001) higher compared with cells in the back subcutaneous and there was
a very significant (p<0.001) decline in the proliferative capacity of cells
with the increase of body weight. The highest TG value and mRNA level of PPARγ
were obtained in the visceral cells from 5000 g fish and the TG value, mRNA
level of PPARγ in cells increased to a greater extent with the increase
of fish body weight, irrespective of the depot origin. Moreover, in the case
of the same weight of fish, there was a greater differentiation degree in visceral
cells than in back subcutaneous cells. In summary, preadipocyte lipogenic capacity
of growing fish depends mainly on fat depot origin and significantly greater
lipogenic capacity of the fish’s adipose tissue is localized visceral.
Furthermore, the proliferation capacity of fat cell declines with the increase
of body weight and the increasing weight during the growth of healthy fish enhances
the lipogenic capacity. Researchers conclude that fat cells from different regions
and body weight are inherently distinct. |
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Yang Gao
,
Da-wei Hu
,
Lai-jun WangJing
and
Shuai Yang
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Path planning for mobile robot with blind zone, caused by
limited sensing capability, is a difficult and practical problem, from which
most local path planning approach is suffering. In this study, entry point has
introduced to represent the free road which may guide the robot to find the
gaps between obstacles. So that, the priory detected free road which has fallen
in to blind zone, could be memorized and a new local path planning approach
is proposed. By the memorizing, the historical sensor information, is infact
partly memorized. By tracking the entry point in blind zone and estimating the
probability distribution using uncented kalman filter, the influence of blind
zone is reduced. All entry points are then evaluated using a evaluate function.
So that both the current sensor information and the historical sensor information
are making used. Compared with the traditional local path planning approaches,
this approach avoid the trap problem and the hover problem came with the blind
zone. Simulations have proved the effect. |
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Yang Gao
,
Kelly Colletti
and
Gregory S. Pari
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Human cytomegalovirus (HCMV) UL84 is a phosphoprotein that shuttles from the nucleus to the cytoplasm and is required for oriLyt-dependent DNA replication and viral growth. UL84 was previously shown to interact with IE2 (IE86) in infected cells, and this interaction down-regulates IE2-mediated transcriptional activation in transient assays. UL84 and IE2 were also shown to cooperatively activate a promoter within HCMV oriLyt. UL84 alone can interact with an RNA stem-loop within oriLyt and is bound to this structure within the virion. In an effort to investigate the binding partners for UL84 in infected cells, we pulled down UL84 from protein lysates prepared from HCMV-infected human fibroblasts by using a UL84-specific antibody and resolved the immunoprecipitated protein complexes by two-dimensional gel electrophoresis. We subsequently identified individual proteins by matrix-assisted laser desorption ionization-tandem time of flight analysis. This analysis revealed that UL84 interacts with viral proteins UL44, pp65, and IE2. In addition, a number of cell-encoded proteins were identified, including ubiquitin-conjugating enzyme E2, casein kinase II (CKII), and the multifunctional protein p32. We also confirmed the interaction between UL84 and IE2 as well as the interaction of UL84 with importin α. UL44, pp65, and CKII interactions were confirmed to occur in infected and cotransfected cells by coimmunoprecipitation assays followed by Western blotting. Ubiquitination of UL84 occurred in the presence and absence of the proteasome activity inhibitor MG132 in infected cells. The identification of UL84 binding partners is a significant step toward the understanding of the function of this significant replication protein. |
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