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Articles by Yan Wang
Total Records ( 28 ) for Yan Wang
  Yan Wang and Jin Zhou
  The hazards of endocrine-disrupting chemicals (EDCs) in natural ecosystems are increasingly drawing public attention. These chemical agents affect aquatic life and its proliferation and can eventually cause reduced population density and species biodiversity. At present, EDCs are being considered as possible reasons for the functional degradation of aquatic ecosystems. Field and laboratory studies suggest that EDCs can disrupt the reproductive and developmental health of various species. Molecular evidence shows that EDCs exhibit genotoxicity and can destroy genetic diversity and structure. Evolutionary toxicology further demonstrates that EDCs can affect the pattern of natural selection and eventually lead to evolutionary potential (or adaptability) loss. Historical statistics and ecological risk estimation provided additional robust data to prove that EDCs contribute to population density and biomass decline. Therefore, EDCs may be a latent cause of aquatic species loss or extinction. In the present article, the extinction risk attributable to EDCs are briefly reviewed and discussed based on the aforementioned factors. The present study aims to assess the species vulnerability to extinction under EDCs exposure and provide a scientific basis for making policy decisions on wildlife resource conservation.
  Ling-Bin Liu , Di-Yan Li , Xiao-Ling Zhao , Yi-Ping Liu , Yan Wang and Qing Zhu
  Prolactin Receptor (PRLR), as an important regulatory gene about growth and differentiation, might be a candidate gene for reproductive traits. The purpose in the present study was to analyze the association of Single Nucleotide Polymorphisms (SNPs) of exon 6 in prolactin receptor gene with egg production traits in Erlang mountainous chickens, a native breed from Poultry farm of Sichuan Agricultural University which consisted of two pure lines SD02 and SD03. Polymerase Chain Reaction-single-strand Conformation Polymorphism (PCR-SSCP) and DNA sequencing methods were used. Results showed that One SNP (G1836C) which had not yet been reported was found and two alleles (G, C) and two genotypes (GG, CC) were also detected. It observed significant differences in allelic and genotypic frequencies among all the chicken populations. Genotype frequencies in all chicken for GG and CC were 56.22 and 43.78%, respectively. Associations of polymorphisms of the PRLR gene with egg production traits were analyzed by using a GLM procedure. The CC genotype had extremely significant effect on Age at First Egg (AFE) (p<0.01) and the AFE of CC genotype had 3.75 days earlier than GG genotype. There was no significant association of G1836C with BWFE; body weight at first egg, WFE; weight at first egg, EN; egg number during 300 days after hatch, BWTA; body weight at 300 days of age and WTE; egg weight at 300 days of age. Therefore, the study showed that the PRLR gene may either a major gene that influences age at first egg of chicken or a molecular marker in close linkage with such a gene.
  Juan Li , Cen Wang , Fan-Li Kong , Yan Wang , Xiao-Ling Zhao , Yi-Ping Liu and Qing Zhu
  MUSTN1 (Mustang, Musculoskeletal Temporally Activated Novel-1) gene is a musculoskeletal temporally activated novel gene which plays a key role in regulating the muscle development. In this study, the Single Nucleotide Polymorphisms (SNPs) in MUSTN1 were scanned, the association of SNPs with carcass traits in chickens were analyzed by Polymerase Chain Reaction-single-strand Conformation Polymorphism (PCR-SSCP) and DNA sequencing methods. Two variations (2120T>C and g.2160A>G) were identified when screened its entire exons and partial introns in 638 chickens. The genotypes of g.2120T>C (Mutations in exon 2) were significantly associated with breast muscle weight, muscle fiber diameters (p<0.05). The genotypes of g.2160A>G (Mutations in exon 2) were significantly associated with breast muscle weight, breast muscle weight percentage (p<0.05) and great significantly associated with muscle fiber diameters (p<0.01). MUSTN1 may act as a candidate gene of quantitative trait loci in regulating muscle growth.
  Yan Wang , Huadong Meng and Xiqin Wang
  For multi-target tracking it is difficult to obtain target trajectories from measurements of close targets and clutter. The Gaussian Mixture Probability Hypothesis Density (GMPHD) as a closed form solution for the Probability Hypothesis Density (PHD) filter can easily provide track labels of targets in clutter. But when targets are too close to each other, such as crossing and occluded conditions, the GMPHD tracker can’t resolve identities of the targets which affects and even interferes with the decision of the commander. Based on the separation distance we proposed, delayed merging GMPHD tracker is proposed to correctly track close targets in clutter. Simulation results show that our proposed approach significantly improves the tracking performance of the GMPHD filter for correctly identifying targets in close proximity.
  Zhijun Xiao , Ye Zhang , Yan Wang and Feng Xu
  A lot of systematic reviews/meta-analyses compiled by Chinese professionals and published in Chinese medical journals bring a lot of perplexity as they help to a certain extent to make decision in some conflicting clinical results. This study aims to assess the quality of systematic reviews and meta-analyses on antidepressant therapy published in Chinese journals. The reviews/meta-analyses on antidepressant therapy were identified by searching three main Chinese data banks i.e., Chinese National Knowledge Infrastructure (CNKI), Wanfang Data (WF) and Chinese Biomedical Literature Database (CBM). A pre-stated criterion was used for review/meta-analysis selection. All reviews were evaluated by two reviewers separately. Data in qualified reviews were extracted into a Microsoft Excel database for analysis. Two assessment tools were used: (1) the Overview Quality Assessment Questionnaire (OQAQ) and (2) Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA). Sixty eight reviews/meta-analyses were included in this study. More than 50% of them had methodological and reporting defects which could have reduced the reliability of the review/meta-analysis results. The flaws were mainly low quality of search strategy, inappropriate bias control and absence of quality assessment for original study.
  Yan Wang and Zhiping Li
  Objectives: It was well known that the utilizations of antiepileptic drugs had been shown in many countries, such as Italy, Netherlands, Dutch and so on. However, those in China were rarely disappeared. In this study, the AEDs using pattern in clinic for children at Shanghai of China were studied. Moreover, the application of monotherapy and polytherapy of AEDs was analyzed to assess whether drug mono and polytherapy are rational or not. Methods: In this study, 8160 prescriptions of total 1,483,061 children aged 0-18 years in the Children’s Hospital of Fudan university on the diagnosis of epilepsy were retrieved from July, 2014 to October, 2015. Prescribing pattern of AEDs and the individual AEDs applied to monotherapy and polytherapy on using rate and dosage were analyzed. Besides, the utilization of valproic acid (VPA), levetiracetam (LEV) and topiramate (TPM) prescribed to different ages of children were covered. Results: Children aged from 2-11 years were the most frequency in the prescriptions with 74.07%. The VPA solution, LEV tablet, oxcarbazepine (OX) tablet and TPM were in the highest frequency used both in monotherapy and polytherapy. Meanwhile, proportion of older and newer AEDs on polytherapy arrived at 59.83 and 53.84%, which was higher than those of monotherapy with 18.67 and 39.63%. Furthermore, the dosage was increased from 9.50±0.17 to 11.49±0.34 mL in VPA group, from 4.79±0.1 to 5.61±0.19 mL in LEV group and from 59.45±2.46 to 77.34±3.06 mg in TPM with the number of medication added on polytherapy, which were significantly higher than monotherapy. Conclusion: The usage of newer AEDs in children was all-too-frequency at Shanghai of China, while polytherapy was most commonly used in Chinese children and certain different dose between monotherapy and polytherapy was found in VPA, LEV and TPM.
  Yan Wang and Zhiping Li
  Background and Objectives: CYP3A5*3 with higher frequency was found to affect the metabolisms of many drugs such as tacrolimus and maraviroc and was proved to be one of the major factors influencing the inter-individual discrepancy in different races. In the present study, the effect of CYP3A5*3 on the plasma concentration and efficacy of valproic acid (VPA) was analyzed to explore the role of CYP3A5*3 in the inter-individual discrepancy. Methodology: A total of 64 children with epilepsy who administered by VPA were recruited. Then, serum VPA concentrations were measured by direct chemiluminescence assay and the polymorphism of CYP3A5 (rs776746) was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Difference among the CYP3A5 allele on dose, concentration, adjusted concentration (AC), concentration-dose ratio and efficacy of VPA was analyzed by one-way ANOVA or t test. Results: Doses for GG carriers were significantly higher than those for AG carriers (p = 0.037). Moreover, both AC and concentration-dose ratio in patients carrying GG genotype were lower than those in AG type patients (p = 0.049, p = 0.001). However, there was no statistical difference in the frequency of CYP3A5*3 type among controlled, improved and uncontrolled-seizure groups (p = 0.9). Conclusion: The GG genotypes could decrease the AC and concentration-dose ratio of VPA which might provide a potential mechanism underlying inter-individual discrepancy of VPA, however, CYP3A5*3 did not influence the efficacy of VPA.
  Yan Wang , Lina Wang , Guomin Xu and Dehua Wei
  Background and Objective: Gestational diabetes mellitus (GDM) are known as the increased risk of postpartum type 2 diabetes. It is believed that the efficient maternal glycemic control of GDM could not decrease the neonatal morbidity from serious complications. Thus, this study was aimed to investigate the protective effect of hesperidin against streptozotocin (STZ) induced gestational diabetes mellitus (GDM) rat models and its possible mechanism. Materials and Methods: The STZ was used for the induction of diabetes mellitus for the pregnant rats and received the hesperidin to 19 days. On the 19th days (pregnant rats), the blood samples and fetal rats of all group rats were collected, weighted the fetal rats and placental was estimated. Serum advanced glycation end products (AGEs) was estimated in the heart and brain of pregnant rats at regular interval. The mRNA expression of NOX2, MCP-1, RAGE, p65, VEGF, VCAM-1 and RAGE were estimated in the placenta. Results: Dose-dependent treatment of hesperidin significantly (p<0.001) altered the placental weight, fetal rats weight, serum blood glucose, lipid level, glycogen and serum insulin level. Hesperidin treatment slightly, but not considerably reduces the probability of fetal development defects as compared to GDM group. Conclusion: Hesperidin showed the potential effect on fetal development in pregnant rats with gestational diabetes mellitus via AGEs-RAGE signalling pathway.
  Huadong Yin , Zhichao Zhang , Xi Lan , Xiaoling Zhao , Yan Wang and Qing Zhu
  The MyoD gene family has been proposed to profoundly modulate muscle development and carcass performance in farm animals. In this study, researchers examined Single Nucleotide Polymorphisms (SNPs) in the exons of the Myf5, Myf6 and MyoG genes using Polymerase Chain Reaction (PCR)-Single Strand Conformation Polymorphisms (SSCP) and DNA sequencing methods in 360 individuals from 6 commercial pure lines of Sichuan Daheng meat type quality chickens. About 2 SNPs (87T>C and 96C>T) in exon 1 of Myf5, 1 SNP (154T>C) in exon 1 of MyoG and no variation in Myf6 were detected. The 96C>T SNP in Myf5 was a rare variant and was not analyzed further. The association analysis of genotypes with carcass traits revealed that the genotypes of SNP (87T>C) in Myf5 were significantly associated with Live Weight (LW), Carcass Weight (CW), Semi-Eviscerated Weight (SEW) and Eviscerated Weight (EW) (p<0.05). The SNP genotypes (154T>C) in MyoG were significantly associated with Live Weight (LW), Carcass Weight (CW), Eviscerated Weight (EW) and Breast Muscle Weight (BMW) (p<0.05). The results suggested that Myf5 and MyoG genes are potential major genes or are in close linkage disequilibrium with the QTL affecting carcass traits in this population of chickens. The 2 SNPs may potentially have use as markers for Marker-Assisted Selection (MAS) in chicken breeding.
  Huadong Yin , Yan Wang , Xiaoling Zhao , Shiyi Chen , Zhichao Zhang , Yiping Liu and Qing Zhu
  Fatty deposition in organism is an important factor which influences meat quality. Previously, Heart Fatty Acid-Binding Protein (H-FABP) gene was studied as a candidate gene which having effect on Intramuscular Fat content (IMF). In current study, we chose four SNPs loci (g.260T>C, g.675G>A, g.783C>T and g.1198T>C) of H-FABP which have been proved to have distinct correlation with IMF as molecular markers to scan SNPs in a new breeding Chinese yellow-feather broiler population. Researchers allocated adaptive individuals into high-IMF group and low-IMF group according to the haplotypes which were constructed of genotypes at four SNP sites of each individual in F1 generation and these haplotypes had been proved to be the preponderant or recessive haplotype. The core group was chose from the F1 generation based on the traditional breeding method. And then, we detected the genotypes of the four loci by SNPs screening and analyzed the selection effect in F2 generation which were obtained from mating and crossing within each group. Results indicated that the preponderance alleles were changed at Locus 1, 3 and 4 in the three groups of F2 and there were no significant differences in IMF among the three groups (p>0.05). However, the IMF of the core group and the low fat group was higher than which of high fat group. The result of variance analysis showed that there were no significant difference in the effect of the different genotypes of the four SNPs on IMF (p>0.05). The four loci on the H-FABP gene in the research were not suitable as candidate markers to select IMF in this yellow-feather broiler strain.
  Yan Wang , Hao Qu , Cheng-Long Luo , Jie Wang and Ding-Ming Shu
  Large deletion or mutation at the exon 10 and 3' untranslated region of Growth Factor Receptor (GHR) gene results in dwarf chicken and the common method for detecting mutation is direct PCR amplification. But since, the target fragment is large, the amplification is inefficient and unreliable and this process consumes a lot of expensive specific DNA polymerases. Researchers focused on the point mutation C146280A in the deletion fragment and set up a new method to detect large deletion and point mutation in this region. Comparing with direct PCR this new method which can detect both large deletion and point mutation with regular low-cost DNA polymerases is more stable, reliably and cheapness.
  Hengyong Xu , Yan Wang and Jiwen Wang
  Fat is the main composition of the body, it not only has an important role to maintain the animal’s physiological function but also its precipitation and sedimentary parts have became the key factor to influence the carcass and meat quality. As two members of the family of the fatty acid transport proteins, FATP1 and FATP4 involved in the transport of long chain fatty acids across the plasma membrane and the metabolism of fatty acids. They are the key genes which have an influence on fat content and maintain skin phospholipids metabolism. So, in this study, the characteristic of structure, function and variation of the gene FATP1 and FATP4 have been summarized. Researchers hope this content can provide certain reference for the researchers in the future.
  Guo-Dong Yang , Dan-Ju Kang , Yan-Hong Li , Jin-Cheng Li , Yan Wang , Xiang-Ya Kong , Qin-Fan Li and Jian-Hua Wang
  Endophytic Undifilum oxytropis found within locoweeds produces the poisonous alkaloid swainsonine which is responsible for locoism in grazing animals. A modified enzymatic assay for swainsonine in the mycelia of Undifilum fungi was developed. The standard equation was Y = 0.5169X- 0.1129 (regression coefficient r = 0.9985). The calibration graph of swainsonine was linear in the range 0.05-0.5 μg mL-1. The method was validated for linearity, precision and accuracy. The modified method has been applied to explore the swainsonine accumulation properties in the endophytic Undifilum fungi in liquid media. Under the described culture condition, the mycelia weight reached a maximum value on the 20th day whereas the peak of swainsonine biosynthesis took place on the 28th day. The result indicated that the increment of mycelium weight and the swainsonine accumulation in the endophytic Undifilum fungi is not completely synchronous.
  Xun Wang , Hengmin Cui , Haifeng Liu , Ling Zhao , Xi Peng , Hengyong Xu , Yan Wang , Yuhao Wang , Ling Niang and Qian Rong
  To study the effect of the crude polysaccharides from Poria cocos (PCCP) on mRNA expression of adipogenic factors during 3T3-L1 preadipocyte differentiation. 3T3-L1 preadipocytes were induced to differentiate with MDI (3-isobutyl-1-Methylxanthine, Dexamethasone and Insulin) induction media contained PCCP (50 μg mL-1) and cultured for 8 days. The extent of the differentiation was assessed by Nile Red staining and GPDH activity. In addition, researchers used real-time quantitative PCR to analyzed the expression of genes related to adipocyte differentiation. The results showed that treatment of differentiating 3T3-L1 preadipocytes with PCCP (50 μg mL-1) resulted in an increase in the expression of the adipocyte genes such as Fatty Acid System Synthase (FAS), adipocyte fatty acid binding Protein (aP2), insulin-responsive Glucose Transporter (GLUT4) and transcriptional factor genes such as CCAAT/Enhancer Binding Protein α (C/EBPα), peoxisome Proliferator-Activated Receptor gamma (PPARγ) and Sterol Regulatory Element-Binding Protein 1c (SREBP-1c) to some degree. Overall, the data suggested that there was the tendency of increasing the expression of genes related to adipocyte differentiation in PCCP treated cells.
  Yue Yin , Xi Lan , Kai Tian , Tian-Jie Li , Qing Zhu , Yi-Ping Liu , Xiao-Ling Zhao , Hua-Dong Yin , Yao-Dong Hu and Yan Wang
  Interferon-β (IFN-β) and immunoglobulin M (IgM), immunoglobulin G (IgG) are essential in anti-retrovirus progress. ALV-J is a kind of ubiquitous retrovirus in chicken. To investigate whether natural Avian Leukosis Virus strain J (ALV-J) infection has effect on the concentrations of serum IFN-β, IgM and IgG, two important time points after hatching, 3 and 18 weeks age which represent the early and late stages of ALV-J infection, respectively were decided to study. The results reveal that the serum IFN-β, IgM and IgG increase at the early stage of ALV-J infection while decrease at the late stage compared with the control chickens which indicate that ALV-J infection boosts the immune response and up-regulates IFN-β and antibody while long-term infection suppresses immune function and down-regulates IFN-β and antibody.
  Xiufang Wang , Yan Wang , Hongbo Bi and Running Gao
  At present, a massive portion of data stored in the heat-supply network management system has formed the data grave which does not embody the intrinsic properties of data. To solve this problem, it is particularly important to take effective mining methods which reuse the existing historical data to improve the current system. In this paper, we first dispersed the continuous attribute information based on both entropy and importance of attribute to preprocess the data of heat-supply network. Then we exploited three kinds of algorithm for data mining, namely, classification and prediction based on the decision tree, cluster analysis based on the K-mean partition and association rules mining based on the frequent itemset model. Finally, we established forecasting model combining the results of three aforementioned mining schemes. The model was then embedded into the prediction module of present system and the results demonstrated the proposed scheme can improve the prediction performance efficiently.
  Quan-Guo Zhai , Jing-Ping Niu , Man-Cheng Hu , Yan Wang , Wen-Juan Ji , Shu-Ni Li and Yu-Cheng Jiang
  Two 2-D metal-organic coordination polymers, {[Ag(NH2-BPT)] · NO3}n (1) and {[Ag(BPT)] · H2O}n (2), have been synthesized via self-assembly of AgNO3 and 4-amino-3,5-bis(3-pyridyl)-1,2,4-triazole (NH2-BPT) under hydrothermal conditions by controlling the reaction temperatures. Lower reaction temperature (140°C) led to formation of 1, which crystallizes in the monoclinic system, space group C2/c, a = 24.001(3), b = 15.844(2), and c = 12.981(3) Å, V = 2996.8(6) Å3, Z = 8. When the temperature was increased to 180°C, in situ deaminization of the organic ligand led to crystallization of 2 (space group P21/n, a = 7.3106(10), b = 19.633(2), and c = 9.0596(16) Å, V = 1190.2(3) Å3, Z = 4). The NH2-BPT in 1 and BPT in 2 are μ4 tetradentate utilizing two triazolyl and two pyridyl nitrogens, generating an unusual 2-D layer, in which binuclear Ag(I) motifs and organic ligands are four-connecting nodes that inter-link in 4462 topology. Adjacent 2-D metal-organic layers are linked by a system of hydrogen bonds to form 3-D supramolecular frameworks. Strong blue fluorescence emissions are observed for 1 and 2 in the solid state at ambient temperature.
  Shu-Yun Shi , Jia-Ning Xu , Wen-Ying MA , Xiao-Bing Cui , Yan Wang , Guo-Wu Wang and Ji-Qing Xu
  Two new polyoxometalates [HN(C2H5)3]3[PMo12O40] (1) and [NH2(C2H4NH3)2]2[Mo5P2O23] (2) have been hydrothermally synthesized and characterized by IR, UV-Vis, XPS spectra, TG analyses, and single crystal X-ray diffraction. Crystal data for 1: monoclinic, space group C2/c, a = 24.651(5) Å, b = 10.822(2) Å, c = 22.413(5) Å, β = 120.37(3)°, and V = 5158.7(18) Å3; for 2: triclinic, space group P-1, a = 9.821(2) Å, b = 9.948(2) Å, c = 14.687(3) Å, α = 95.490(3)°, β = 98.870(3)°, γ = 95.710(3)°, and V = 1401.7(5) Å3. The crystal structure analyses reveal complex hydrogen bonds in both 1 and 2 in 3-D supramolecular arrays constructed from different polyoxoanions and organic ligands.
  Yan Wang , Ling Ye , Hong Ding , Tie-Gang Wang , Guo-Wu Wang , Shu-Yun Shi , Xiao-Bing Cui and Ji-Qing Xu
  Two compounds based on polyoxometalates, (4,4'-H2bpy)7(4,4'-Hbpy)[BW12VIO40]2[BW12VIO40] ·10H2O (1) and (4,4'-H2bpy)6(4,4'-bpy)[P2W18VIO62]2 · 18H2O (2), have been prepared under hydrothermal conditions and characterized by IR, XPS spectra, TG analyses, and single crystal X-ray diffraction. Both compounds consist of 4,4'-bipyridine units and polyoxometalates: [BW12VIO40]5- for 1 and [P2W18VIO62]6- for 2. In addition, 1 contains both Keggin-type and disordered Keggin-type anions, while 2 contains a (H2O)10 water cluster.
  Yan Wang , Hong Li , Qiyi Tang , Gerd G. Maul and Yan Yuan
  Herpesvirus lytic DNA replication requires both the cis-acting element, the origin, and trans-acting factors, including virally encoded origin-binding protein, DNA replication enzymes, and auxiliary factors. Two lytic DNA replication origins (ori-Lyt) of Kaposi's sarcoma-associated herpesvirus (KSHV) have been identified, and two virally encoded proteins, namely, RTA and K8, have been shown to bind to the origins. In this study, we sought to identify cellular factors that associate with ori-Lyt by using DNA affinity purification and mass spectrometry. This approach led to identification of several cellular proteins that bind to KSHV ori-Lyt. They include topoisomerases (Topo) I and II, MSH2/6, RecQL, poly(ADP-ribose) polymerase I (PARP-1), DNA-PK, Ku86/70 autoantigens, and scaffold attachment factor A (SAF-A). RecQL appears to associate with prereplication complexes and be recruited to ori-Lyt through RTA and K8. Topoisomerases, MSH2, PARP-1, DNA-PK, and Ku86 were not detected in prereplication complexes but were present in replication initiation complexes on ori-Lyt. All these cellular proteins accumulate in viral replication compartments in the nucleus, indicating that these proteins may have a role in viral replication. Topo I and II appear to be essential for viral DNA replication as inhibition of their activities with specific inhibitors (camptothecin and ellipticine) blocked ori-Lyt-dependent DNA replication. Furthermore, inhibition of PARP-1 with chemical inhibitors (3-aminobenzamide and niacinamide) resulted in decreased ori-Lyt-dependent DNA replication, whereas hydroxyurea, which raises PARP-1 activity, caused an increase in the DNA replication, suggesting a positive role for PARP-1 in KSHV lytic DNA replication.
  Yan Wang , Camille Le Ray , Francois Audibert and Marie- Soleil Wagner
 

BACKGROUND: Nuchal cords are rarely associated with significant neonatal morbidity or mortality.

CASE: A primigravida with a normal term pregnancy presented for diminished fetal movement. Several nuchal cord loops were found on the ultrasound imaging, with normal Doppler findings. Abnormal fetal heart rate pattern appeared after 6 hours of labor. A cesarean delivery was performed. A healthy baby was born with eight nuchal cord loops.

CONCLUSION: The management of nuchal cord with a single loop, suspected before delivery, seems clear: it should not influence the clinical management. However, in this case, the previous knowledge of multiple nuchal loops may have influenced our decision to perform a cesarean delivery.

  Qingling Li , Hui Zhang , Yan Wang , Bo Tang , Xin Liu and Xiaocong Gong
  A versatile programmable eight-path-electrode power supply (PEPS) system for manipulating microfluids of a complex microfluidic chip has been developed. The PEPS system consisted of a single chip microprocessor as a central control unit and a personal computer (PC) as an upper computer, and the program could be operated under Windows98/2000/XP. The voltage output of each electrode was in the range of 0 to +8000 V (0.1% precision) while the current output was in the range of 0 to +999 μA. The voltage of eight electrodes could be operated either independently or synchronously by random combination of any electrodes through switching. The voltage output modes were “switch-off/floating”, “switch-on” and “grounded” and fast switched at ms-level between these modes, and run time (0.1 s precision) of these modes could be controlled as desired. The PEPS system was conveniently for controlling flow rate and direction of electroosmotic flow (EOF) in a chip network. Six electrodes were chosen to control the repeated ‘injection and separation’ of 1.0 x 10–5 M fluorescein isothiocyanate (FITC) in a six-reservoir glass-based chip. The relative standard deviation (R.S.D., n = 4, S/N = 10) of the repeated operation was 0.9% for the reservation time (tR) and 2.3% for the peak height, respectively.
  Jun Zhang , Shurong Wang , Yan Wang , Mijuan Xu , Huijuan Xia , Shoumin Zhang , Weiping Huang , Xianzhi Guo and Shihua Wu
  In this work, we report the large-scale synthesis of SnO2 nanoparticles with sizes in the range of 5–15 nm via a well-established metal alkoxide hydrolysis route. This solution method proves to be facile and environmentally benign without using any toxic organic solvent, surfactant and special laboratory apparatus. The morphology, size and phase of the SnO2 nanoparticles were characterized by transmission electron microscopy (TEM), selected area electron diffraction (SAED) and X-ray diffraction (XRD). A gas sensor was fabricated from the prepared SnO2 nanoparticles and applied to ethanol sensing test as well as some other gases. Obtained results show that the sensor exhibited high sensitivity, quick response and good selectivity to ethanol and n-butanol at an optimum operating temperature of 220 °C.
  Jun Zhang , Shurong Wang , Yan Wang , Mijuan Xu , Huijuan Xia , Shoumin Zhang , Weiping Huang , Xianzhi Guo and Shihua Wu
  ZnO hollow spheres were successfully prepared by using carbon microspheres as templates and characterized by X-ray diffraction (XRD) analysis, scanning electron microscope (SEM), transmission electron microscope (TEM) and selected area electron diffraction (SAED). A gas sensor was fabricated from the as-prepared ZnO hollow spheres and tested to different concentrations of NH3 and NO2 at different operating temperatures. The results showed that the ZnO hollow-sphere sensor exhibited extremely different sensing behaviors to NH3 and NO2. The optimum operating temperature of the sensor was 220 °C for NH3 and 240 °C for NO2, respectively. At 220 °C, the responses to 25, 50 and 75 ppm NH3 were 7.9, 11.1 and 20.4 and the response times were as long as several minutes. At 240 °C, the responses to 10, 50 and 100 ppm NO2 were 140.6, 172.8 and 286.8 and the corresponding response times were 31, 19 and 9 s. In addition, it is also shown that the gas sensor exhibited much higher response to NO2 than to other gases at 240 °C, implying the good selectivity and potential application of the sensor for detecting NO2.
  Chao Wang , Jianwu Zhao , Yan Wang , Nan Lou , Qiang Ma and Xingguang Su
  Stable, monodisperse CdTe quantum dots (QDs) capped with mercaptosuccinic acid were prepared facilely in aqueous medium, which were deposited on the quartz slides to form multilayer films by electrostatic interactions with poly(dimethyldiallyl ammonium chloride) (PDDA). The PDDA/QDs multilayer films are easily fabricated and show high photostability. The fluorescence of the multilayer films could be quenched effectively by Hg (II) ions without obvious shift in maximum photoluminescence emission wavelength, which can be used for the detection of Hg (II) ions. The electron transfer process between the capping ligands and Hg (II) ions should be mainly responsible for the remarkable quenching effect of Hg (II) ions. Under optimal conditions, the quenched fluorescence intensities of multilayer films are perfectly described by modified Stern–Volmer equation with the concentration of Hg (II) ions ranging from 0.01 μM to 1 μM.
  Li Jiang , Junmei Fan , Li Bai , Yan Wang , Yu Chen , Lu Yang , Liangyi Chen and Tao Xu
  Insulin-stimulated GLUT4 translocation to the plasma membrane constitutes a key process for blood glucose control. However, convenient and robust assays to monitor this dynamic process in real time are lacking, which hinders current progress toward elucidation of the underlying molecular events as well as screens for drugs targeting this particular pathway. Here, we have developed a novel dual colored probe to monitor the translocation process of GLUT4 based on dual color fluorescence measurement. We demonstrate that this probe is more than an order of magnitude more sensitive than the current technology for detecting fusion events from single GLUT4 storage vesicles (GSVs). A small fraction of fusion events were found to be of the "kiss-and-run" type. For the first time, we show that insulin stimulation evokes a ~40-fold increase in the fusion of GSVs in 3T3-L1 adipocytes, compared with basal conditions. The probe can also be used to monitor the prefusion behavior of GSVs. By quantifying both the docking and fusion rates simultaneously, we demonstrate a proportional inhibition in both docking and fusion of GSVs by a dominant negative mutant of AS160, indicating a role for AS160 in the docking of GSVs but not in the regulation of GSV fusion after docking.
  Yupeng Chen , Lei Shi , Lirong Zhang , Ruifang Li , Jing Liang , Wenhua Yu , Luyang Sun , Xiaohan Yang , Yan Wang , Yu Zhang and Yongfeng Shang
  SOX genes encode a family of high-mobility group transcription factors that play critical roles in organogenesis. The functional specificity of different SOX proteins and the tissue specificity of a particular SOX factor are largely determined by the differential partnership of SOX transcription factors with other transcription regulators, many of which have not yet been discovered. Virtually all members of the SOX family have been found to be deregulated in a wide variety of tumors. However, little is known about the cellular and molecular behaviors involved in the oncogenic potential of SOX proteins. Using cell culture experiments, tissue analysis, molecular profiling, and animal studies, we report here that SOX2 promotes cell proliferation and tumorigenesis by facilitating the G1/S transition and through its transcription regulation of the CCND1 gene in breast cancer cells. In addition, we identified β-catenin as the transcription partner for SOX2 and demonstrated that SOX2 andβ-catenin act in synergy in the transcription regulation of CCND1 in breast cancer cells. Our experiments not only determined a role for SOX2 in mammary tumorigenesis but also revealed another activity of the multifunctional protein, β-catenin.
  Yan Wang , Eugene C. Dow , Yao-Yun Liang , Rajesh Ramakrishnan , Hongbing Liu , Tzu-Ling Sung , Xia Lin and Andrew P. Rice
  Cdk9 is the catalytic subunit of a general RNA polymerase II elongation factor known as positive transcription elongation factor b (P-TEFb). The kinase function of P-TEFb requires phosphorylation of Thr-186 in the T-loop of Cdk9 to allow substrates to access the catalytic core of the enzyme. To identify human phosphatases that dephosphorylate the T-loop of Cdk9, we used a Thr-186-phosphospecific antiserum to screen a phosphatase expression library. Overexpression of PPM1A and the related PPM1B greatly reduced Cdk9 T-loop phosphorylation in vivo. PPM1A and Cdk9 appear to associate in vivo as the proteins could be co-immunoprecipitated. The short hairpin RNA depletion of PPM1A resulted in an increase in Cdk9 T-loop phosphorylation. In phosphatase reactions in vitro, purified PPM1A could dephosphorylate Thr-186 both with and without the association of 7SK RNA, a small nuclear RNA that is bound to ∼50% of total cellular P-TEFb. PPM1B only efficiently dephosphorylated Cdk9 Thr-186 in vitro when 7SK RNA was depleted from P-TEFb. Taken together, our data indicate that PPM1A and to some extent PPM1B are important negative regulators of P-TEFb function.
 
 
 
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