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by
Yan Song |
Total Records (
1 ) for
Yan Song |
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Chun-Jiang Zhang
,
Wei Li
,
Hong-Yu Li
,
Yu-Ling Wang
,
Tian Yun
,
Zheng-Peng Song
,
Yan Song
and
Xing-Wen Zhao
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Extracts from five Tibetan medicinal plants collected from the Tibetan Plateau
were evaluated for antiviral activity against herpes simplex virus type 2 (HSV-2)
in vitro and in vivo. Viral plaque reduction assays showed that extracts
from four out of five plants inhibited HSV-2 infection significantly with 50%
effective concentrations (EC50) values ranging from 0.35 ± 0.11
to 1.83 ± 0.21 mg/mL. The other plant, Swertia mussotii Franch. (Gentianaceae),
exhibited activity in inhibiting the viral biosynthesis. In the attachment assay,
two plants, Dracocephalum heterophyllum Benth. (Lamiaceae) and Dracocephalum
tanguticum Maxim. (Lamiaceae) reduced the attachment of HSV-2 to cell surface.
Interestingly, all of the extracts showed virucidal activity. Analyzed by real-time
PCR, three extracts showed strong inhibition of HSV DNA replication with Dracocephalum
heterophyllum and Dracocephalum tanguticum at the concentration of
4 mg/mL and Lagotis brevituba Maxim. (Scrophulariaceae) at 1 mg/mL. BALB/c
mice were used for determining in vivo efficacy. Mice encephalitis herpes
models were established by infection with HSV-2. The extracts of Dracocephalum
heterophyllum, Dracocephalum tanguticum, and Swertia mussotii
at a dose of 1 g/kg per day significantly prolonged the mean survival times and
reduced the mortality of HSV-2 infected mice compared with control group (P <
0.05). Taken together, we conclude that the antiviral mechanisms of these
plants involve various stages of virus replication. Extracts from three of these
plants, Dracocephalum heterophyllum, Dracocephalum tanguticum, and
Swertia mussotii, may be possible candidates in developing anti-HSV-2 medicine. |
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