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Articles by Yan Ren
Total Records ( 3 ) for Yan Ren
  Fei Guo , Yuanzhi Wang , Chuangfu Chen , Hui Zhang , Jun Qiao , Yan Ren , Junbo Zhang and Zhiqiang Li
  TFSS is an important virulence factor of Brucella, organized as one operon containing 12 different across the cell wall bacterial proteins among which VirB5 regulates the host phagocytosis of Brucella and the transportation of Brucella in the host cells. This study has constructed cDNA library from Brucella melitensis 16M-infected murine macrophage Raw264.7, identified and confirmed the interaction between Brucella VirB5 and FTH1 of RAW264.7 using yeast two-hybrid and Co-Immunoprecipitation (Co-IP) technologies. Subsequently, the morphological changes and the expression of apoptosis-related genes in Brucella-infected RAW264.7 cells have been investigated with Electron Microscope (EM) and real-time quantitative RT-PCR, respectively. The present study has demonstrated that VirB5 and FTH1 play important roles in intracellular parasitism of Brucella and inhibition of FHT1 expression accelerates the apoptosis of macrophage.
  Wenge Hu , Shiwei Ma , Chuangfu Chen , Yuanzhi Wang , Yan Ren , Xudong Cao and Jinliang Sheng
  Leuciscus merzbacheri is a unique vulnerable indigenous fish which is only distributed in the Junggar Basin, Xinjiang. In this study, researchers cloned two L. merzbacheri β-actin promoter fragments of different length: SZ11 and SZ21 and analyzed their structural features. The mammalian expression vector pEGFP-N1 was used to construct eukaryotic expression vectors β1 pEGFP-N1-AFP III and β2 pEGFP-N1-AFP III in which fish type III antifreeze protein gene was used as the structural gene. The results showed that the cloned two fragments of β-actin promoters had the ability to drive the expression of the green fluorescent protein gene in BHK-21 cells. These data suggest that the vectors researchers constructed based on β-actin promoter could be exploited as all fish recombinant eukaryotic expression vector.
  Sheng-Jun Dai , Li Shen and Yan Ren
  Two new eudesmane-type sesquiterpenoids, lyratol A-B (1 and 2), were isolated from the whole plant of Olanum lyratum Thunb and their structures were elucidated by extensive spectroscopic analyses. In addition, two new compounds were found to show significant cytotoxicity against selected cancer cells in vitro, including P-388 and HT-29.
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