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Articles by Y.O. Fasina
Total Records ( 3 ) for Y.O. Fasina
  Y.O. Fasina , E.T. Moran , C.M. Ashwell , D.E. Conner , M. Leslie and S.R. Mckee
  Day-old Ross 308 chicks were used to evaluate the effect of dietary gelatin on intestinal development and broiler chick performance. Chicks were randomly allocated to two dietary treatments. The first treatment was the control SB diet based on corn-soybean meal and second treatment was the SBG test diet that contained 2% added gelatin with the total sulfur amino acid level remaining the same as in SB. Each treatment consisted of 4 replicate pens with 14 chicks per pen. Total duration of experiment was 0-3 weeks of age. Chick performance (weight gain and feed conversion efficiency) was evaluated on day 7 and 14. To assess intestinal development, abundance of mRNA of selected enterocyte genes (alkaline phosphatase, leucine aminopeptidase and mucin) was quantitatively determined by RT-PCR on day 7. Biochemical indices including DNA concentration, protein:DNA and protein:RNA ratios were also used to assess intestinal development. Results indicated that chicks fed the SBG diet had improved feed conversion efficiency on day 7 (p<0.05) and higher body weight gain on day 21 (p<0.05) compared to chicks fed the SB diet. Furthermore, eneterocyte genes assessed were upregulated (p<0.05) in the duodenum of SBG chicks. Jejunal protein:RNA ratio was also higher for SBG chicks (p<0.05), indicating a higher rate of protein synthesis in this tissue. The non-essential amino acids provided by gelatin beyond the most limiting appear to enhance early intestinal development and chick growth.
  Y.O. Fasina and Y.L. Olowo
  Yeast products contain nucleotides that are essential for cellular functions and growth and may therefore enhance intestinal villi morphology and growth performance. Accordingly, two 42-day experiments were conducted to evaluate the efficacy of Maxigen® (a novel yeast-based product) in enhancing intestinal villi morphology, growth performance and flock uniformity. Experiment 1 utilized 480 (day-old) female chicks obtained from a commercial hatchery, weighed and randomly assigned to 2 dietary treatments. Treatment 1 (CX, control) consisted of chicks fed corn-soybean meal (SBM) diet that was not supplemented with Maxigen®. Treatment 2 (MG) consisted of chicks fed corn-SBM basal into which Maxigen® was added at 0.075% level. Each treatment consisted of 12 replicate pens, with each pen housing 20 chicks. On days 21 and 42 of experiment, growth performance (body weight and feed conversion) and flock uniformity were assessed. Experiment 2 was conducted in a similar manner, except that male chicks were used. Intestinal tissue samples were also collected on day 10 of experiment for histological evaluation of villi morphology. From the results, only Experiment 2 showed differences between CX and MG treatments for the parameters assessed. Specifically, at day 42, feed conversion ratio of birds in MG treatment (1.67) was superior (P<0.05) to that of birds in CX treatment (1.71). In addition, flock uniformity of MG birds (66.4%) was better than the uniformity of CX flock (56.8%; P = 0.0527). It was concluded that Maxigen® supplemented at 0.075% level of the diet enhanced growth performance and flock uniformity of broiler chickens.
  Y. Jababu , C. Blue , P.R. Ferket and Y.O. Fasina
  Background and Objectives: Spray-dried plasma (SDP) is a bioactive feed additive that frequently improves broiler growth performance and may therefore replace antibiotic growth promoters (AGPs). To improve our understanding of SDP mechanisms of action, a 2-week experiment was conducted to compare the potency of porcine SDP and bacitracin methylene disalicylate (BMD) antibiotic to stimulate intestinal development in neonate chicks. Materials and Methods: Day-old (288) Ross 708 broiler male chicks were obtained from a commercial hatchery and randomly assigned to 6 treatments in a completely randomized design. Treatments consisted of chicks given unmedicated corn-soybean meal basal diet containing no BMD or SDP (CX), at 0.055 g kg–1 diet (MX), SDP at 10 g kg–1 diet (SP1), SDP at 20 g kg–1 diet (SP2), SDP at 30 g kg–1 diet (SP3) and SDP at 40 g kg–1 diet (SP4). On day 3, 7 and 14 of experiment, intestinal maltase activity was determined. Villi morphometrics was also measured in the jejunum (d 7). Results: On day 7, all chicks that consumed SDP and BMD had lower villus: crypt ratio (p = 0.0006) and higher goblet cell density (p < 0.0001) compared to CX. Furthermore, on day 14, ileal maltase activity was higher for all chicks that consumed SDP (3.036-3.065 μg glucose/min/mL/μg protein, p < 0.0001) compared to CX (3.025 μg glucose/min/mL/μg protein). Conclusion: Like BMD antibiotic, dietary SDP at 30 or 40 g kg–1 diet improves feed conversion ratio in chicks in-part, by increasing ileal maltase activity, reducing intestinal villus/crypt ratio (indicative of ongoing villi renewal/regeneration) and increasing goblet cell density.
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